Bioimaging Unit

Leica DMi8

Leica DMi8

Modalities: invert, widefield, live cell

Overview

A fully automated widefield system for fluorescence and brightfield imaging.

The DMi8 is a fully automated widefield system for fluorescence and brightfield imaging. It is equipped with a full range of objectives, monochrome and colour cameras and filtersets to enable fluorescence imaging of up to four distinct channels from UV to far red excitation.

Stage and z are also motorised, allowing multichannel z stacks in multiple positions or tilescans, plus in combination with an Okolab on-stage incubator, live cell imaging is also possible.

Imaging Modes:

  • 2D (xy) or 3D (xyz), multichannel with timelapse and/or tiling and/or multiple locations

Specification

Here you can find the specification for this microscope.

Objectives

Magnification

NA

Coverslips

Other Modes

Immersion

5 0.15      Air
10  0.4  0.17    Air
20  0.8  0.17    Air
40  0.85  0.17    Air
40  1.3 0.17   Oil
63  1.4  0.17    Oil
100  1.44  0.17  DIC  Oil

 

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube

Example Fluorophores

Excitation

Dichroic

Emission

Part Number

Leica DAPI DAPI, Hoechst 325-375 400 435-485  
Leica GFP GFP, FITC,AF488 450-490   500-550  
Leica Rhod Cy3, AF555 541-551   565-605  
Leica TXR* AF568, AF594 540-580 585 592-668  
Leica CY5 CY5, AF647 560-650   662-738  
Leica mK-Red mK-red 550-570   602-682  
Leica mK-Green mK-green 420-460   602-682  

 

 

 

 

 

 

 

 

 

 *fitted on request

Light Sources

  • Lumencor SOLA SE365 LED light engine for epifluorescence
  • White LED for transmission

Cameras

  • Hamamatsu Flash 4 v2, (monochrome 4MP sCMOS)
  • Leica DFC450 (colour 5MP CCD)

Location

Biogerontology Building, Room F20

Health Innovation Neighbourhood
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use.