Leica TCS SP2 (Invert)
The Leica TCS SP2 is a point scanning confocal microscope, based around an inverted DMIREB stand. The system has three photomultipliers for fluorescence imaging with an additional one for transmitted light, which includes DIC. It can allow users a wide choice of fluorochrome dyes including: FITC, TRITC, TEXAS RED and CY5. Additional transmitted and reflected light modes are available. Live-cell experimentation on this system is possible through the use of a stage-top live-cell incubation chamber where both temperature and CO2 concentration can be set and monitored.
The operating software offers FRAP and FRET with built in ‘Wizards’ to help new users, and in addition has basic quantification and colocalisation modules. 3D projections (stereo anaglyphs) and movies can also be saved.
Specifications
Objectives: 63x (Oil, 1.32), 40x (air, 0.7), 20x (air, 0.5), 10x (air, 0.3).
Imaging modes: Multi point x,y (2D) with tiling, volume x,y,z (3D), time lapse x,y,z,t (4D), live-cell.
Laser lines: Multi line Argon; 458nm, 476nm, 488nm, 514nm, HeNe; 543nm, HeNe; 633
Location
Dermatology, Room M2.131, William Leech Building, Medical School
