Bio-Imaging Unit

Nikon TiE Multi-Modality

Nikon TiE Multi-Modality

Overview

This system is built around three imaging technologies; Wide field fluorescence, Total Internal reflectance Fluorescence Microscopy (TIRF microscopy) and Spinning Disk confocal Microscopy.

Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells. The system utilises the Yokogawa CSU10 spinning disk head and coupled with either an Andor iXON 885 EMCCD (1k x 1k) camera or Photometrics Evolve EMCCD (512 x 512) camera, both are capable of capturing both high resolution and high speed (>50fps) confocal data sets.

Both cameras can also be used to capture images illuminated by TIRF. Although not confocal imaging, TIRF microscopy improves the image signal to noise ratio by restricting penetration of the excitation light into the specimen. This technique is particularly useful for monitoring processes that happen within a couple of hundred nanometers from the coverslip media interface.

The microscope employs hardware focal drift correction and is fully-enclosed in a darkened, heated environmental chamber with the ability to set, maintain and monitor temperature, humidity, CO2 and O2 levels as imaging conditions dictate.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as FRAP, FRET and colocalisation as well as data quantification.

Specification

Here you can find the specifications for this microscope.

Imaging modes

  • multi point x,y (2D) with tiling
  • volume x,y,z (3D)
  • flourescece
  • DIC
  • time lapse x,y,z,t (4D)
  • TIRF
  • spinning disk confocal

Confocal laser Lines

  • Multi line Argon: 457nm, 476nm, 488nm (TIRF), 514nm
  • 561nm (TIRF)
  • 594nm (TIRF)

Location

This microscope is located in the Central Unit, on campus.

The Central Unit is based in the William Leech Building room M2.141.

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

The minimal booking time for this equipment is 30 minutes. Time increments of 15 minutes are permitted.

Bookings can be cancelled with no charge up to an hour before scheduled use.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.