Bio-Imaging Unit

Nikon A1R (Invert)

Nikon A1R (Confocal)

Overview

The Nikon A1R is a point scanning confocal microscope based around a fully motorized, inverted Nikon Ti body with hardware-enabled focus correction.

The Nikon A1R has three photomultipliers for fluorescence imaging, one for transmitted light detection and a dedicated photomultiplier array for spectral profiling or if required, unmixing of fluorescently ‘complex’ specimens.

Incorporated into the confocal scan-head are both resonant (fast scanning) and non-resonant (conventional scanning) scanning mirrors. This allows experimenters to have the option of imaging in high resolution (up to 4096x4096 pixels), at high speed (420fps at 512x32 pixels) or a combination of both.

This arrangement is ideal for live-cell photo-activation and photo-bleaching studies where resonant scanning can be used to capture images at high-speed and non-resonant scanning can be used to target a region of interest for photo-activation.

Resonant scanning mode has added benefits; for live-cell experiments, photo-sensitive specimens remain live for longer and fluorophore bleaching is significantly reduced. The system will also perform regular 2D, 3D and 4D imaging.

The microscope is fully-enclosed in a heated environmental chamber and has the ability to set, maintain and monitor temperature, humidity, CO2 and O2 levels as imaging conditions dictate.

The system is run through Nikon’s versatile Elements software package and incorporates wizards to perform common imaging tasks such as FRAP, FRET and colocalisation as well as data quantification.

Specification

Here you can find the specifications for this microscope.

Objectives

  • CFl Plan Fluor DL-10x F/0.3 Ph1
  • CFl Plan Apochromat VC 20x/0.75
  • CFl Super Plan Fluor ELWD ADM 40x C/0.60 Ph2
  • CFl Plan Apochromat VC 60x/1.4 Oil N2
  • CFl Plan Apochromat VC 100x/104 Oil N2

Imaging modes

  • Multi point x,y (2D) with tiling
  • volume x,y,z (3D)
  • time lapse x,y,z,t (4D) spectral
  • Live Cell

Excitation

  • 405nm
  • Multi line Argon: 457nm, 476nm, 488nm, 514nm
  • 561nm
  • 647nm

Detection

  • 2 x PMT
  • 2 x GaAsP
  • 1x PMT for transmission

Location

This microscope is located in the Central Unit, on campus.

The Central Unit is based in the Leech Building room M2.154.

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.