Zeiss AxioObserver
Zeiss AxioObserver Spinning Disk
Modalities: invert, confocal, live cell
Overview
Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.
The system utilises the Yokogawa CSUX1 spinning disk head and coupled with a QuantEM camera (Photometrics) capable of capturing high speed (>30fps) confocal data sets.
The microscope employs software autofocus correction and is fully-enclosed in a heated environmental chamber with the ability to set, maintain and monitor:
- temperature
- humidity
- O2 levels
- CO2 levels
as imaging conditions dictate. The system is run through Zeiss’ AxioVision software package.
Imaging modes
- Multipoint x,y with tiling (2D),
- volume x,y,z (3D),
- fluorescence,
- FRET,
- DIC, t
- ime-lapse x,y,z,t (4D)
Specification
Here you can find the specifications for this microscope.
Objectives
Magnification |
NA |
Coverslip |
Other modes |
Immersion |
10 | 0.3 | 0.17 | Air | |
20 | 0.8 | Air | ||
40 | 1.3 | 0.17 | Oil | |
100 | 1.4 | 0.17 | Oil |
Filters (epi)
Cube |
Example fluorophores |
Excitation |
Dichroic |
Emission |
Part Number |
DAPI | DAPI, Hoechst | ||||
FITC |
FITC+AF488 |
||||
TRITC |
Laser lines
405 nm, 488nm, 561nm, 639 nm
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Booking
Find out how to book this microscope.
Booking information
- The minimal booking time for this system is 30 minutes.
- Time increments of 15 minutes are permitted.
- Bookings can be cancelled with no charge up to an hour before scheduled use.