Bio-Imaging Unit

Zeiss AxioObserver

Zeiss AxioObserver Spinning Disk confocal (invert)

Overview

Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.

The system utilises the Yokogawa CSUX1 spinning disk head and coupled with a QuantEM camera (Photometrics) is capable of capturing high speed (>30fps) confocal data sets.

The microscope employs software autofocus correction and is fully-enclosed in a heated environmental chamber with the ability to set, maintain and monitor:

  • temperature
  • humidity
  • O2 levels
  • CO2 levels

as imaging conditions dictate. The system is run through Zeiss’ AxioVision software package.

Specification

Here you can find the specifications for this microscope.

Imaging modes

Multipoint x,y with tiling (2D), volume x,y,z (3D), fluorescenceFRETDIC, time-lapse x,y,z,t (4D)

Example filters

  • DAPI
  • FITC
  • TRITC

Example objectives

  • 100x (oil, 1.4)
  • 40x (oil, 1.3)
  • 20x (air, 0.8)
  • 10x (air, 0.3)

Laser lines

  • 405 nm
  • 488nm
  • 561nm
  • 639nm

Location

This equipment is located in the Campus for Ageing and Vitality.

It is in the Ageing Research Labs room F. 14b.

Campus for Ageing and Vitality
Newcastle University
Westgate Rd
Newcastle upon Tyne
NE4 9AQ

Here, you can get directions to the building.

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

The minimum booking time for this system is 30 minutes. Time increments of 15 minutes are permitted.

Before booking this equipment, please ensure the following:

  • any users are fully trained, and approved by a member of BioImaging facility staff
  • microscopes are left in a good state of tidiness (lenses/stage should be cleaned of any oil)
  • filters and lenses must not be removed or switched between systems without authorisation from Bioimaging facility staff

If you work at Newcastle University, you can book this microscope online by using iLab.

If you're a commercial company or an academic at another institution, just contact us to discuss your requirements.