Bioimaging Unit

Zeiss AxioImager (System 2)

Zeiss AxioImager with Apotome (System 2)

Modalities: upright, widefield

Overview

The Zeiss AxioImager with Apotome (System 2) is a high quality wide field imaging system.

The Zeiss AxioImager is a high quality wide field imaging system that offers three modes of operation:

  • brightfield transmitted light (phase and DIC)
  • wide field fluorescence
  • apotome structured illumination (confocal like) fluorescence

The system utilises two separate camera systems for colour and monochrome imaging.

The Apotome (structured illumination) mode allows users to collect confocal-like images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.

A range of fluorescent filters are available which cover the commonly used dyes:

  • DAPI
  • FITC
  • TRITC
  • CY5

The illumination source is a Colibri LED.

The microscope has as a motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture. 

Analysis of data/images can be carried out using the Zen blue software which includes quantification and colocalisation modules. 

Imaging modes

  • Wide field fluorescence
  • Brightfield
  • Multi-point x,y (2D) with tiling
  • Volume x,y,z (3D)
  • Structured illumination (Apotome, fluorescence only)
  • Phase contrast
  • DIC 
Axio2_2022

Specification

Here you can find the specifications for this microscope.

 Objectives

Magnification

NA

Coverslip

Other modes

Immersion

5 0.16     Air
10 0.45 0.17 Ph1 Air
20 0.8 0.17 Ph2 Air
40 0.95 0.17 Ph3 Air
63 1.4 0.17 DIC Oil
100 1.4 0.17   Oil

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part Number

Zeiss 49 DAPI, Hoechst 335-385 395 420-470  
Zeiss 38 HE GFP, FITC, AF488 450-490 495 500-550  
Zeiss 23 FITC 475-495 500 515-530  
  TRITC 540-552 560 580-630  
Zeiss 63 HE AF568, mCherry, TRITC 560-584 590 598-660  
Zeiss 50 CY5, AF647 625-655 660 665-715  

 

 

 

 

 

 

 

 

 

Light source (epi)

Colibri 1- LED fluorescent light (365,470,555,625)

Detectors (cameras)

Flash 4 camera

Zeiss 105 (Colour for brightfield HC)

Location

William Leech Building, room M2.149

Newcastle University
William Leech Building
Medical School
Framlington Place 
Newcastle upon Tyne
NE2 4HH

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 

Training

Here you find useful training videos for the system.

Zen Blue software tutoial. 

Explore Zen.