Zeiss Axio Imager II
The Zeiss AxioImager is a high quality imaging system that offers three modes of operation:
- widefield transmitted light
- widefield fluorescence
- Apotome structured illumination (confocal like) fluorescnece
The system utilises two separate camera systems, colour and monochrome, each having a resolution of 1388x1040 pixels. They are setup to have the same final magnification and field of view, allowing bright field and fluorescence images to be easily overlaid. The widefield transmitted light imaging mode has both phase contrast and DIC and is available on most lenses.
The Apotome (structured illumination) mode allows users to collect Confocal images and ‘Z’ stacks, which can subsequently be projected as 3D images (stereo anaglyphs) and movies.
A range of fluorescent filters are available which cover the commonly used dyes, DAPI, FITC,TRITC and CY5. The illumination source is a wide spectrum alignment free metal halide lamp.
The microscope has as an autofocus system and a precision motorised stage which enables users to automatically acquire tiled images from large specimens (or from an entire slide) and stitch them together post capture.
Analysis of data/images can be carried out using the AxioVision software which includes quantification and colocalisation modules.
Specifications
Objectives: 100x (oil, 1.46), 63x (oil, 1.4), 40x (oil, 1.3), 40x (air, 0.75), 25x (multi, 0.8), 10x (air, 0.45), 2.5x (air, 0.75).
Imaging modes: Multi-point x,y (2D) with tiling, volume x,y,z (3D), Structured illumination (Apotome), fluorescence, Phase, DIC.
Location
Bio-Imaging Unit, Room M2.154, William Leech Building, Medical School
