Dr Ian Singleton
Marie Curie International Outgoing Fellow

  • Email: ian.singleton@ncl.ac.uk
  • Telephone: +44 (0) 191 208 3043
  • Address: School of Biology,
    Ridley Building,
    Newcastle University,
    Newcastle upon Tyne
    NE1 7RU

Background

BSc, University of Sheffield, UK
PhD University of Sheffield, UK
1989-1995. Lecturer in University College Dublin
1995-2000. Lecturer in Adelaide University, Australia
1999-2000 Visiting Research Associate, University of Liverpool
2000- present. Lecturer in School of Biology, University of Newcastle upon Tyne. Senior lecturer from 2005.

From Sept 2011 to Feb 2014 I was in receipt of a Marie Curie International Outgoing Fellowship (EC FP7 funded) to study the diversity of ectomycorrhizal fungi using next generation sequencing (in collaboration with Prof Tom Bruns, UC Berkeley, USA)

Research Interests

My academic background is in applied and environmental microbiology and I am particularly interested in microbial interactions with plants and pollutants. My current research work covers areas such as microbial spoilage of fresh produce, plant/fungal associations and metal interactions with plants and their associated microbes.

1. Fresh produce spoilage by microbes : It is estimated that up to 30% of fresh produce (fruit, vegetables etc) can be lost due to microbial spoilage and I am working with plant scientists to develop novel methods (with a focus on ozone gas exposure) to reduce this spoilage. Currently we are examining microbial populations that exist on the surface of various types of vegetable produce and determining if ozone gas exposure is able to reduce microbial loads present. I am also interested in how ozone affects the growth of microbes (particularly fungi). 

2. Fungal associations with plants : I am currently in receipt (Sept 2011 - Feb 2014) of a Marie Curie International Outgoing Fellowship (EC FP7 funding) to study the diversity of ectomycorrhizal fungi associated with pine. This is being carried out in collaboration with Prof. Tom Bruns (UC Berkeley, USA) and involves the use of next generation DNA sequencing techniques.

3. Microbe/pollutant/plant interactions : Recent research projects have examined the interactions between cadmium, plants and microbes (in collaboration with Dr. Annie Borland, School of Biology, Newcastle University). e.g. cadmium uptake by plants, the effect of Cd on plant rhizosphere microbial populations and the use of microbes to enhance growth of plants in Cd contaminated soil. I am also interested in the effect of metal nanoparticles on microbes.

Postgraduate Supervision

Current postgraduates :

Shreya Wani : Novel methods to reduce spoilage of fresh vegetable produce (HDC funded project). Co-supervised with Prof. Jerry Barnes.

Joao Fernandes (part-time overseas student based in Lisbon): Rapid nucleic acid based methods to detect E. coli in environmental samples. Co-supervised with Prof Jerry Barnes

Hartati Oktarina : The effect of silver nanoparticle contamination on beneficial soil fungi. Co-supervised with Prof. Anne Borland.

Hamida Idris : Actinomycete diversity in Atacama desert soils (Main supervisor : Prof Mike Goodfellow)

 

Previous students in Newcastle:

James Clapp: Pollutant exposure biomarkers in birds. In collaboration with Dr. R. Bevan (Newcastle)

Phattanawan Pronmin : Co-supervised with Dr. Anne Borland (Newcastle)

Hakima Althalb : Bioremediation of oil contaminated sandy soils. In collaboration with the Libyan Petroleum Research Institute, Tripoli, Libya. 

Louise Pease : NERC CASE award with Astra Zeneca. Toxicogenomics of yeast. Co-supervised by Ian Head (Newcastle), Simon Avery (University of Nottingham) and Jason Snape (Astra Zeneca). Completed PhD in January 2011.

Zoe Keatinge : NERC funded student. Development of novel methods to assess the bioavailability of contaminants in soil
Collaboration with Professor Faith Williams and Dr. Elaine Mutch, Medical School, University of Newcastle. Completed PhD 2006

Chris Malkin : NERC funded student. Microbial spoilage of fresh produce. Collaboration with Dr. Jerry Barnes, University of Newcastle. Completed 2008

Nikos Tsortzakis : Greek government funded PhD student. Microbial spoilage of produce. Collaboration with Dr. Jerry Barnes, Dr Tahir Taybi and Dr. Anne Borland, University of Newcastle. Completed 2006

Eva Tallentire : Industry funded PhD student : Novel methods to decontaminate fresh food produce. Co-supervised with Dr. Jerry Barnes. Completed 2007

Elisabeth Clare Lowe : Main supervisor is Clive Butler (University of Exeter. BBSRC studentship on Microbial diversity of selenate reducers in soil. Completed 2008.

Esteem Indicators

Member of SETAC UK Council (2003-2006)
Invited on Editorial Board of Journal of Environmental Sciences (China)
Co founder of BioFresh Ltd
Co-founder of Geneius Laboratories Ltd.
Organised SETAC UK conference in Newcastle Sept 2005.
Council member of the British Mycological Society : 2007 to 2010

Funding

Research : I have been involved in approximately £1 million of external funding from a variety of sources including UK research councils (BBSRC, NERC and EPSRC), industry and the EC (EC Asia-Link award and EC Marie Curie International Outgoing Fellowship).

Examples of funding received :

BBSRC funding (2003-2005). Bioavailability of benzo(a)pyrene in soil
Collaboration with CEFAS, UK and University of Aberdeen

BBSRC studentship in collaboration with Dr. Clive Butler, University of Exeter. Does the diversity of selenate reducers in soil affect selenium availability. Sept 2004- Sept 2007.

KTP projects :

1. Potential commercial applications of ozone. Collaboration with Prof J. Barnes (Newcastle) and JLA Ltd, Ripponden. 2006-2008

2. Commercial uses of ozone in the food industry. Collaboration with Prof J. Barnes and Mack Multiples Ltd. 2006-2008

BBSRC China Partnership Award : £24,900 from 2004-2008

Industrial Relevance

Co-founder of Geneius Laboratories Ltd : a company using both traditional microbiology and molecular biology techniques to identify microbes from food and environmental samples.  For further details see : www.geneiuslabs.com

Projects

Undergraduate Teaching

Stage 1 : Contributor to BIO1004 (Microbiology) and BIO1008 (tutorials)
Stage 2 : Contributor to BIO2017 (Microbiology 2) and ACE 2041 (Food Microbiology)
Stage 3 : Contributor to BIO3007 : Pollution of Air, Water and Soil
Stage 3 : Module leader for BIO3020 : Bioremediation
Supervise several undergraduate research projects per year

Postgraduate Teaching

Degree Programme Director for MSc Industrial and Commercial Biotechnology
Module leader for BIO8010 : Bioremediation
Supervise several MSc research projects per year