Newcastle University

Memberships

Physiological Society
British Pharmacological Society

Research Interests

Intestinal epithelial transport; nutrient and drug transporters; regulation of epithelial transport by neurohormonal mechanisms and components of diet; intracellular pH regulation; membrane transport in neuroblastoma cells.

• Thwaites DT. News from the dark side!. Journal of Physiology 2012, 590(24), 6255-6256.
• Thwaites DT, Anderson CMH. The SLC36 family of proton-coupled amino acid transporters and their potential role in drug transport. British Journal of Pharmacology 2011, 164(7), 1802-1816.
• Edwards N, Anderson CMH, Gatfield KM, Jevons MP, Ganapathy V, Thwaites DT. Amino acid derivatives are substrates or non-transported inhibitors of the amino acid transporter PAT2 (SLC36A2). Biochimica et Biophysica Acta: Biomembranes 2011, 1808(1), 260-270.
• Anderson CMH, Thwaites DT. Hijacking solute carriers for proton-coupled drug transport. Physiology 2010, 25(6), 364-377.
• Anderson CMH, Jevons M, Thangaraju M, Edwards N, Conlon NJ, Woods S, Ganapathy V, Thwaites DT. Transport of the photodynamic therapy agent 5-aminolevulinic acid by distinct H⁺-coupled nutrient carriers co-expressed in the small intestine. Journal of Pharmacology and Experimental Therapeutics 2010, 332(1), 220-228.
• Anderson CMH, Howard A, Walters JRF, Ganapathy V, Thwaites DT. Taurine uptake across the human intestinal brush-border membrane is via two transporters: H⁺-coupled PAT1 (SLC36A1) and Na⁺- and Cl^--dependent TauT (SLC6A6). Journal of Physiology 2009, 587(4), 731-744.
• Anderson CMH, Ganapathy V, Thwaites DT. Human solute carrier SLC6A14 is the beta-alanine carrier. Journal of Physiology 2008, 586(17), 4061-4067.
• Thwaites DT, Anderson CMH. Deciphering the mechanisms of intestinal imino (and amino) acid transport: The redemption of SLC36A1. Biochimica et Biophysica Acta: Biomembranes 2007, 1768(2), 179-197.
• Thwaites DT, Anderson CMH. H⁺-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine. Experimental Physiology 2007, 92(4), 603-619.
• Anderson CMH, Thwaites DT. Regulation of intestinal hPepT1 (SLC15A1) activity by phosphodiesterase inhibitors is via inhibition of NHE3 (SLC9A3). Biochimica et Biophysica Acta: Biomembranes 2007, 1768(7), 1822-1829.
• Abbot EL, Grenade DS, Kennedy DJ, Gatfield KM, Thwaites DT. Vigabatrin transport across the human intestinal epithelial (Caco-2) brush-border membrane is via the H⁺-coupled amino-acid transporter hPAT1. British Journal of Pharmacology 2006, 147(3), 298-306.
• Kennedy DJ, Raldua D, Thwaites DT. Dual modes of 5-(N-ethyl-N-isopropyl)amiloride modulation of apical dipeptide uptake in the human small intestinal epithelial cell line Caco-2. Cellular and Molecular Life Sciences 2005, 62(14), 1621-1631.
• Anderson CMH, Thwaites DT. Indirect regulation of the intestinal H⁺-coupled amino acid transporter hPAT1 (SLC36A1). Journal of Cellular Physiology 2005, 204(2), 604-613.
• Miyauchi S, Abbot EL, Zhuang L, Subramanian R, Ganapathy V, Thwaites DT. Isolation and function of the amino acid transporter PAT1 (SLC36A1) from rabbit and discrimination between transport via PAT1 and system IMINO in renal brush-border membrane vesicles. Molecular Membrane Biology 2005, 22(6), 549-559.
• Kennedy DJ, Gatfield KM, Winpenny JP, Ganapathy V, Thwaites DT. Substrate specificity and functional characterisation of the H⁺/amino acid transporter rat PAT2 (SLC36A2). British Journal of Pharmacology 2005, 144(1), 28-41.
• Anderson CMH, Grenade DS, Boll M, Foltz M, Wake KA, Kennedy DJ, Munck LK, Miyauchi S, Taylor PM, Campbell FC, Munck BG, Daniel H, Ganapathy V, Thwaites DT. H⁺/amino acid transporter 1 (PAT1) is the imino acid carrier: An intestinal nutrient/drug transporter in human and rat. Gastroenterology 2004, 127(5), 1410-1422.
• Anderson CMH, Mendoza ME, Kennedy DJ, Raldua D, Thwaites DT. Inhibition of intestinal dipeptide transport by the neuropeptide VIP is an anti-absorptive effect via the VPAC₁ receptor in a human enterocyte-like cell line (Caco-2). British Journal of Pharmacology 2003, 138(4), 564-573.
• Chen Z, Fei Y-J Y, Anderson CMH, Wake K, Miyauchi S, Huang W, Thwaites DT, Ganapathy V. Structure, function and immunolocalization of a proton-coupled amino acid transporter (hPAT1) in the human intestinal cell line Caco-2. Journal of Physiology 2003, 546(2), 349-361.
• Chen Z, Kennedy DJ, Wake KA, Zhuang L, Ganapathy V, Thwaites DT. Structure, tissue expression pattern, and function of the amino acid transporter rat PAT2. Biochemical and Biophysical Research Communications 2003, 304(4), 747-754.
• Boll M, Foltz M, Anderson CMH, Oechsler C, Kottra G, Thwaites DT, Daniel H. Substrate recognition by the mammalian proton-dependent amino acid transporter PAT1. Molecular Membrane Biology 2003, 20(3), 261-269.
• Thwaites DT, Kennedy D, Raldua D, Anderson CMH, Mendoza ME, Bladen C, Simmons N. H⁺/dipeptide absorption across the human intestinal epithelium is controlled indirectly via a functional Na⁺/H⁺ exchanger. Gastroenterology 2002, 122(5), 1322-1333.
• Kennedy DJ, Leibach FH, Ganapathy V, Thwaites DT. Optimal absorptive transport of the dipeptide glycylsarcosine is dependent on functional Na⁺/H⁺ exchange activity. Pfluegers Archiv: European Journal of Physiology 2002, 445(1), 139-146.
• Kennedy DJ, Thwaites DT. Optimal dipeptide uptake across the apical membrane of human intestinal Caco-2 cell monolayers is dependent on functional NHE3 activity. Journal of Physiology 2002, 539(supplement), 10P-11P.
• Glanville M, Kingscote S, Thwaites DT, Simmons NL. Expression and role of sodium, potassium, chloride cotransport (NKCC1) in mouse inner medullary collecting duct (mIMCD-K2) epithelial cells. Pfluegers Archiv: European Journal of Physiology 2001, 443(1), 123-131.
• Thwaites DT, Basterfield L, McCleave P, Carter S, Simmons NL. Gamma-aminobutyric acid (GABA) transport across human intestinal epithelial (Caco-2) cell monolayers. British Journal of Pharmacology 2000, 129(3), 457-464.
• Christie GR, Thwaites DT. Isolation and functional expression of the mouse intestinal dipeptide transporter, mPepT1. In: JOURNAL OF PHYSIOLOGY-LONDON. 2000.
• Kennedy DJ, Raldua D, Simmons NL, Thwaites DT. Optimization of dipeptide uptake at the apical membrane of human intestinal Caco-2 cell monolayers requires co-operative activity of the di/tripeptide transporter hPepT1 and the Na+-H+ exchanger NHE3. In: JOURNAL OF PHYSIOLOGY-LONDON. 2000.
• Thwaites DT, Raldua D, Simmons NL. Regulation of dipeptide uptake in Caco-2 cell monolayers by vasoactive intestinal peptide and guanylin. In: JOURNAL OF PHYSIOLOGY-LONDON. 2000.
• Marcaggi P, Thwaites DT, Deitmer JW, Coles JA. Chloride-dependent transport of NH₄⁺ into bee retinal glial cells. European Journal of Neuroscience 1999, 11(1), 167-177.
• Thwaites DT, Ford D, Glanville M, Simmons NL. H⁺/solute-induced intracellular acidification leads to selective activation of apical Na⁺/H⁺ exchange in human intestinal epithelial cells. Journal of Clinical Investigation 1999, 104(5), 629-635.
• Thwaites DT, Stevens BC. H⁺-zwitterionic amino acid symport at the brush-border membrane of human intestinal epithelial (Caco-2) cells. Experimental Physiology 1999, 84(2), 275-284.
• Ford D, Thwaites D, Simmons N, Gilbert H, Hirst B. Substrate upregulation of the human small intestinal peptide transporter, hPepT1. Journal of Physiology 1998, 507(3), 697-706.
• Thwaites DT. H⁺/amino acid symport in intestinal epithelia. Amino Acids 1997, 13(1), 49.
• Thwaites DT, Markovich D, Murer H, Simmons NL. Na⁺-independent lysine transport in human intestinal Caco-2 cells. Journal of Membrane Biology 1996, 151(3), 215-224.
• Thwaites DT, Cavet M, Hirst BH, Simmons NL. Angiotensin-converting enzyme (ACE) inhibitor transport in human intestinal epithelial (Caco-2) cells. British Journal of Pharmacology 1995, 114(5), 981-986.
• Thwaites DT, Armstrong G, Hirst BH, Simmons NL. D-Cycloserine transport in human intestinal epithelial (Caco-2) cells is mediated by a H⁺-coupled amino acid transporter. British Journal of Pharmacology 1995, 115(5), 761-766.
• Freeman TC, Bentsen BS, Thwaites DT, Simmons NL. H⁺/di-tripeptide transporter (PepT1) expression in the rabbit intestine. Pfluegers Archiv: European Journal of Physiology 1995, 430(3), 394-400.
• Thwaites DT, McEwan GTA, Hirst BH, Simmons NL. H⁺-coupled alpha-methylaminoisobutyric acid transport in human intestinal Caco-2 cells. Biochimica et Biophysica Acta: Biomembranes 1995, 1234(1), 111-118.
• Wenzel U, Thwaites DT, Daniel H. Stereoselective uptake of beta-lactam antibiotics by the intestinal peptide transporter. British Journal of Pharmacology 1995, 116(7), 3021-3027.
• Thwaites DT, McEwan GTA, Simmons NL. The role of the proton electrochemical gradient in the transepithelial absorption of amino acids by human intestinal Caco-2 cell monolayers. Journal of Membrane Biology 1995, 145(3), 245-256.
• Thwaites DT, Hirst BH, Simmons NL. H⁺-coupled solute transport in cultured intestinal epithelia. In: Molecular and Cellular Mechanisms of H⁺ Transport. Heidelberg: Springer-Verlag, 1994, pp.283-296.
• Thwaites DT, McEwan GTA, Brown CDA, Hirst BH, Simmons NL. L-Alanine absorption in human intestinal cells driven by the proton electrochemical gradient. Journal of Membrane Biology 1994, 140(2), 143-151.
• Thwaites DT, Hirst BH, Simmons NL. Substrate specificity of the di/tripeptide transporter in human intestinal epithelia (Caco-2): identification of substrates that undergo H⁺-coupled absorption. British Journal of Pharmacology 1994, 113(3), 1050-1056.
• Thwaites DT, Hirst BH, Simmons NL. Direct assessment of dipeptide/H⁺ symport in intact human intestinal (Caco-2) epithelium: a novel method utilising continuous intracellular pH measurement. Biochemical and Biophysical Research Communications 1993, 194(1), 432-438.
• Thwaites DT, McEwan GTA, Cook MJ, Hirst BH, Simmons NL. H⁺-coupled (Na⁺-independent) proline transport in human intestinal (Caco-2) epithelial cell monolayers. FEBS Letters 1993, 333(1-2), 78-82.
• Thwaites DT, Brown CDA, Hirst BH, Simmons NL. H⁺-coupled dipeptide (glycylsarcosine) transport across apical and basal borders of human intestinal Caco-2 cell monolayers display distinctive characteristics. Biochimica et Biophysica Acta: Biomembranes 1993, 1151(2), 237-245.
• Thwaites DT, McEwan GTA, Brown CDA, Hirst BH, Simmons NL. Na⁺-independent, H⁺-coupled transepithelial beta-alanine absorption by human intestinal Caco-2 cell monolayers. Journal of Biological Chemistry 1993, 268, 18438-18441.
• Thwaites DT, Hirst BH, Simmons NL. Passive transepithelial absorption of thyrotropin-releasing hormone (TRH) via a paracellular route in cultured intestinal and renal epithelial cell lines. Pharmaceutical Research 1993, 10(5), 674-681.
• Thwaites DT, Simmons NL, Hirst BH. Thyrotropin-releasing hormone (TRH) uptake in intestinal brush-border membrane vesicles: comparison with proton-coupled dipeptide and Na⁺-coupled glucose transport. Pharmaceutical Research 1993, 10(5), 667-673.
• Thwaites DT, McEwan GTA, Hirst BH, Simmons NL. Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic. Pfluegers Archiv: European Journal of Physiology 1993, 425(1-2), 178-180.
• Thwaites DT, Brown CDA, Hirst BH, Simmons NL. Transepithelial glycylsarcosine transport in intestinal Caco-2 cells mediated by expression of H⁺-coupled carriers at both apical and basal membranes. Journal of Biological Chemistry 1993, 268(11), 7640-7642.
• McCloy LRR, Taylor PM, Thwaites DT. Neutral amino acid transport via system L at the basolateral membrane of human intestinal Caco-2 cell monolayers. In: Meeting of the Physiological Society. 2001, Sheffield, UK: Wiley-Blackwell Publishing Ltd.
• Anderson CMH, Raldua D, Simmons NL, Thwaites DT. pH-dependent forskolin modulation of beta-alanine uptake at the apical surface of Caco-2 cell monolayers. In: Meeting of the Physiological Society. 2001, Sheffield, UK: Wiley-Blackwell Publishing Ltd.
• Kennedy DJ, Thwaites DT. The selective NHE3 inhibitor S1611 modulates dipeptide transport across human intestinal (Caco-2) cell monolayers. In: Journal of Physiology. 2001, Wiley-Blackwell Publishing Ltd.