Publication:

Interphase molecular cytogenetic screening for chromosomal abnormalities of prognostic significance in childhood acute lymphoblastic leukaemia: a UK Cancer Cytogenetics Group Study (2005)

Author(s): Harrison CJ, Moorman AV, Barber KE, Broadfield ZJ, Cheung KL, Harris RL, Jalali GR, Robinson HM, Strefford JC, Stewart A, Wright S, Griffiths M, Ross FM, Harewood L, Martineau M

  • : Interphase molecular cytogenetic screening for chromosomal abnormalities of prognostic significance in childhood acute lymphoblastic leukaemia: a UK Cancer Cytogenetics Group Study

Abstract: Summary Interphase fluorescence in situ hybridization (iFISH) was used independently to reveal chromosomal abnormalities of prognostic importance in a large, consecutive series of children (n = 2367) with acute lymphoblastic leukaemia (ALL). The fusions, TEL/AML1 and BCR/ABL, and rearrangements of the MLL gene occurred at frequencies of 22% (n = 447/2027) (25% in B-lineage ALL), 2% (n = 43/2027) and 2% (n = 47/2016) respectively. There was considerable variation in iFISH signal patterns both between and within patient samples. The TEL/AML1 probe showed the highest incidence of variation (59%, n = 524/884), which included 38 (2%) patients with clustered, multiple copies of AML1. We were thus able to define amplification of AML1 as a new recurrent abnormality in ALL, associated with a poor prognosis. Amplification involving the ABL gene, a rare recurrent abnormality confined to T ALL patients, was identified for the first time. The use of centromeric probes revealed significant hidden high hyperdiploidy of 33% and 59%, respectively, in patients with normal (n = 21/64) or failed (n = 32/54) cytogenetic results. The iFISH contributed significantly to the high success rate of 91% (n = 2114/2323) and the remarkable abnormality detection rate of 89% (n = 1879/2114). This study highlights the importance of iFISH as a complementary tool to cytogenetics in routine screening for significant chromosomal abnormalities in ALL.

Notes: Journal Article Multicenter Study Research Support, Non-U.S. Gov't England

  • Short Title: Interphase molecular cytogenetic screening for chromosomal abnormalities of prognostic significance in childhood acute lymphoblastic leukaemia: a UK Cancer Cytogenetics Group Study
  • Alternate Journal: British journal of haematology
  • Date: 27-04-2005
  • Journal: British Journal of Haematology
  • Volume: 129
  • Issue: 4
  • Pages: 520-530
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Adolescent Child Child, Preschool *Chromosome Aberrations Core Binding Factor Alpha 2 Subunit Cytogenetic Analysis DNA-Binding Proteins/genetics Fusion Proteins, bcr-abl/genetics Gene Amplification Gene Rearrangement Genes, abl Humans In Situ Hybridization, Fluorescence Infant Interphase Myeloid-Lymphoid Leukemia Protein Oncogene Proteins, Fusion/genetics Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics Prognosis Proto-Oncogenes/genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/genetics

Staff

Professor Anthony Moorman
Professor of Genetic Epidemiology