Publication:

A common core RNP structure shared between the small nucleolar box C/D RNPs and the spliceosomal U4 snRNP (2000)

Author(s): Watkins NJ, Segault V, Charpentier B, Nottrott S, Fabrizio P, Bachi A, Wilm M, Rosbash M, Branlant C, Lührmann R

  • : A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

Abstract: The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5 kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5 kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/ 15.5 kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP.

  • Short Title: A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP
  • Date: 27-10-2000
  • Journal: Cell
  • Volume: 103
  • Issue: 3
  • Pages: 457-466
  • Publisher: Elsevier
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Base Sequence Binding Sites *Evolution, Molecular Fungal Proteins/genetics/metabolism Gene Deletion Hela Cells Human Molecular Weight Nucleic Acid Conformation Precipitin Tests Protein Binding RNA, Fungal/chemistry/genetics/metabolism RNA, Small Nuclear/chemistry/genetics/metabolism RNA, Small Nucleolar/chemistry/genetics/metabolism RNA-Binding Proteins/genetics/*metabolism Recombinant Proteins Regulatory Sequences, Nucleic Acid/genetics Ribonucleoprotein, U4-U6 Small Nuclear/*chemistry/genetics/metabolism Ribonucleoproteins, Small Nucleolar/*chemistry/genetics/isolation & purification/metabolism Spliceosomes/*chemistry/genetics Substrate Specificity Support, Non-U.S. Gov't Yeasts/genetics/*metabolism

Staff

Dr Nick Watkins
Senior Lecturer