Publication:

Processing of vertebrate box C/D small nucleolar RNAs in plant cells (1998)

Author(s): D. J. Leader;G. P. Clark;J. Boag;J. A. Watters;C. G. Simpson;N. J. Watkins;E. S. Maxwell;J. W. Brown

  • : Processing of vertebrate box C/D small nucleolar RNAs in plant cells

Abstract: The recent isolation of a number of plant box C/D small nucleolar (sno)RNAs demonstrates the conservation in plants of sequence and structural elements of processed box C/D snoRNAs. Boxes C and D, and terminal inverted repeats are known to be essential for accumulation and processing in vertebrates and yeast. Processing of vertebrate box C/D snoRNAs was examined by expression of various mouse hsc70 intron 5- U14 constructs in tobacco protoplasts. Full-length U14 and internally deleted U14 accumulated in the plant cells. Human U3 and U8 fragments, consistent with processing to internal box C/C' sequences, also accumulated in the plant cells. The similarity of processing behaviour of the vertebrate box C/D constructs in tobacco protoplasts and Xenopus oocytes suggests the mechanism of processing, involving recognition and association of proteins, is conserved in plants.

  • Short Title: Processing of vertebrate box C/D small nucleolar RNAs in plant cells
  • Journal: Eur J Biochem
  • Volume: 253
  • Issue: 1
  • Pages: 154-60.
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Animal Base Sequence Female Human In Vitro Introns Mice Oligodeoxyribonucleotides/genetics Oocytes/metabolism Plants, Genetically Modified Plants, Toxic RNA Processing, Post-Transcriptional RNA Splicing RNA, Small Nuclear/chemistry/genetics/*metabolism Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Tobacco/genetics/metabolism Xenopus

Staff

Dr Nick Watkins
Senior Lecturer