Publication:

Detecting mismatch repair defects in myeloma (2005)

Author(s): Velangi M, Matheson E, Hall AG, Irving JAE

    Abstract: Defects in the mismatch repair system are associated with a microsatellite unstable phenotype. In this chapter, we describe the preparation of purified plasma cells using CD138 magnetic microbeads as a source of tumor DNA. We also describe a robust, sensitive method for comparing microsatellite repeat units of tumor to constitutive DNA using polymerase chain reaction and laser scanning of fluorescently labeled amplicons in an automated sequencer in order to assess microsatellite instability in myeloma.

      • Book Title: Multiple Myeloma
      • Volume: 113
      • Pages: 269-77
      • Publisher: Humana Press, Inc.
      • Publication type: Book chapter
      • Bibliographic status: Published

      Keywords: Antigens, CD/genetics Base Pair Mismatch/*genetics Base Sequence DNA Primers DNA, Neoplasm/genetics/isolation & purification Electrophoresis, Capillary/methods Humans Membrane Glycoproteins/genetics Microsatellite Repeats Multiple Myeloma/*genetics/immunology Polymerase Chain Reaction/methods Proteoglycans/genetics Research Support, Non-U.S. Gov't

      Staff

      Professor Andy Hall
      Director, NICR

      Dr Julie Irving
      Reader in Experimental Haematology