Publication:

epsilon-sarcoglycan replaces alpha-sarcoglycan in smooth muscle to form a unique dystrophin-glycoprotein complex (1999)

Author(s): Straub V; Ettinger AJ; Durbeej M; Venzke DP; Cutshall S; Sanes JR; Campbell KP

  • : epsilon-sarcoglycan replaces alpha-sarcoglycan in smooth muscle to form a unique dystrophin-glycoprotein complex

Abstract: The sarcoglycan complex has been well characterized in striated muscle, and defects in its components are associated with muscular dystrophy and cardiomyopathy. Here, we have characterized the smooth muscle sarcoglycan complex. By examination of embryonic muscle lineages and biochemical fractionation studies, we demonstrated that epsilon-sarcoglycan is an integral component of the smooth muscle sarcoglycan complex along with beta- and delta-sarcoglycan. Analysis of genetically defined animal models for muscular dystrophy supported this conclusion. The delta-sarcoglycan-deficient cardiomyopathic hamster and mice deficient in both dystrophin and utrophin showed loss of the smooth muscle sarcoglycan complex, whereas the complex was unaffected in alpha-sarcoglycan null mice in agreement with the finding that alpha-sarcoglycan is not expressed in smooth muscle cells. In the cardiomyopathic hamster, the smooth muscle sarcoglycan complex, containing epsilon-sarcoglycan, was fully restored following intramuscular injection of recombinant delta-sarcoglycan adenovirus. Together, these results demonstrate a tissue-dependent variation in the sarcoglycan complex and show that epsilon-sarcoglycan replaces alpha-sarcoglycan as an integral component of the smooth muscle dystrophin-glycoprotein complex. Our results also suggest a molecular basis for possible differential smooth muscle dysfunction in sarcoglycan-deficient patients.

  • Short Title: epsilon-sarcoglycan replaces alpha-sarcoglycan in smooth muscle to form a unique dystrophin-glycoprotein complex
  • Date: 24-09-1999
  • Journal: Journal of Biological Chemistry
  • Volume: 274
  • Issue: 39
  • Pages: 27989-27996
  • Publisher: American Society for Biochemistry and Molecular Biology, Inc.
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Animals Cardiomyopathies/genetics/metabolism/pathology Cricetinae Cytoskeletal Proteins/analysis/deficiency/genetics/ metabolism Dystrophin/ metabolism Embryo Intestines/metabolism/pathology Lung/metabolism/pathology Membrane Glycoproteins/analysis/deficiency/genetics/ metabolism Mice Mice, Inbred mdx Mice, Knockout Muscle, Skeletal/metabolism/pathology Muscle, Smooth/ metabolism/pathology Muscular Dystrophy, Animal/genetics/metabolism/pathology Myocardium/metabolism/pathology Sarcoglycans

Staff

Professor Volker Straub
Harold Macmillan Professor of Medicine