Publication:

Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells (1996)

Author(s): Kremer IB, Hilkens CM, Sylva-Steenland RM, Koomen CW, Kapsenberg ML, Bos JD, Teunissen MB

  • : Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells

Abstract: The capacity of APC to stimulate the proliferation of human peripheral blood T cells decreases upon ultraviolet-B (UVB) irradiation. The aim of this study was to investigate whether all T cell subsets are equally sensitive to this reduced APC function. Established human Th1, Th2, and Th0 clones were stimulated with monocytes in a soluble CD3 mAb-mediated assay that is dependent on the presence of APC. Monocytes were exposed to low nonlethal doses of UVB radiation before coculture with T cells. UVB irradiation inhibited the capacity of monocytes to stimulate the proliferation and IFN-gamma production of Th1 cells in a dose-related fashion. In contrast, UVB-treated monocytes induced normal proliferation and IL-4 production in Th2 cells. Stimulation of Th0 cell proliferation by UVB-irradiated monocytes was normal, but a preferential suppression of IFN-gamma production was observed, thus leading to a more Th2-like cytokine response. The loss of Th1 proliferation upon stimulation with UVB-irradiated monocytes could be overcome by rIL-2; however, IFN-gamma production remained suppressed. IFN-gamma production could be completely restored by rIL-12, whereas the addition of IL-1 beta, TNF-alpha, or indomethacin had no such effect, nor did the addition of mAb to CD28, added to compensate for the reduced B7 expression of UVB-irradiated monocytes. Monocytes exposed to UVB radiation exhibited reduced expression of mRNA for the IL-1 2 subunits p35 and p40 and suppressed production of the IL-12 p70 protein. Our results thus indicate that UVB irradiation of APC selectively impairs Th1-like responses, a phenomenon caused by the UVB-induced suppression of monocyte IL-12 production.

Notes:

  • Short Title: Reduced IL-12 production by monocytes upon ultraviolet-B irradiation selectively limits activation of T helper-1 cells
  • Date: 01-09-1996
  • Journal: Journal of Immunology
  • Volume: 157
  • Issue: 5
  • Pages: 1913-1918
  • Publisher: American Association of Immunologists
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Cytokines/biosynthesis Human *Immunosuppression Interferon Type II/antagonists & inhibitors/biosynthesis/radiation effects Interleukin-12/*biosynthesis/*radiation effects Interleukin-2/pharmacology Lymphocyte Activation/*radiation effects Monocytes/*metabolism/*radiation effects Support, Non-U.S. Gov't Th1 Cells/*immunology/metabolism/*radiation effects Th2 Cells/immunology/metabolism/radiation effects *Ultraviolet Rays

Staff

Dr Catharien Hilkens
Reader in Immunotherapy