Publication:

A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130 (2001)

Author(s): Hilkens CM, Is'harc H, Lillemeier BF, Strobl B, Bates PA, Behrmann I, Kerr IM

  • : A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130

Abstract: The terminal portion of the Janus kinases (Jaks) contains a divergent FERM (Four-point-one, Ezrin, Radixin, Moesin) homology domain comprising 19 conserved hydrophobic regions. To determine the role of this domain in governing recruitment of Jak1, but not Jak3, to the gp130 subunit of the interleukin-6 family of cytokine receptors, the interaction of three Jak1/Jak3 chimeras with gp130 was investigated. Chimeras 1, 2 and 3 (Jak1 FERM regions 1-19, 1-18 and 1-8/Jak3, respectively) were all enzymically active. Chimeras 1 and 2 interacted with the cytoplasmic domain of gp130, although less efficiently than Jak1. Only chimera 2, however, restored gp130 signalling in Jak1-negative cells. The data are consistent with recruitment of Jak1 to gp130 through the Jak1 FERM domain, but also emphasise the likely requirement for precise Jak/receptor orientation to sustain function.

Notes: 0014-5793 Journal Article

  • Short Title: A region encompassing the FERM domain of Jak1 is necessary for binding to the cytokine receptor gp130
  • Date: 2001-08-14
  • Journal: FEBS Letters
  • Volume: 505
  • Issue: 1
  • Pages: 87-91
  • Publisher: Elsevier BV
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence Antigens, CD/*metabolism Binding Sites Cytoplasm/metabolism DNA-Binding Proteins/metabolism Fibrosarcoma Human Membrane Glycoproteins/*metabolism Molecular Sequence Data Protein Structure, Tertiary Protein-Tyrosine Kinase/genetics/*metabolism Recombinant Proteins/genetics/metabolism Signal Transduction Support, Non-U.S. Gov't Trans-Activators/metabolism Tumor Cells, Cultured

Staff

Dr Catharien Hilkens
Reader in Immunotherapy