Publication:

Transcytosis of the polymeric Ig receptor requires phosphorylation of serine 664 in the absence but not the presence of dimeric IgA (1993)

Author(s): Hirt RP; Hughes GJ; Frutiger S; Michetti P; Perregaux C; Poulain-Godefroy O; Jeanguenat N; Neutra MR; Kraehenbuhl JP

  • : Transcytosis of the polymeric Ig receptor requires phosphorylation of serine 664 in the absence but not the presence of dimeric IgA

Abstract: MDCK cells expressing the polymeric immunoglobulin (poly-Ig) receptor, cocultured with IgA-producing hybridoma cells, transported dimeric IgA (dIgA) from the basolateral into the lumenal compartment, where it was recovered as secretory component-dIgA complexes. The tail of the receptor was phosphorylated on serines 664 and 726. Each serine was mutated to alanine. Appearance of A726 receptor at the basolateral surface was reduced approximately 5-fold. This was accompanied by a approximately 5-fold reduction in dIgA transcytosis. Basolateral delivery of receptor was not affected by mutation A664, and in the absence of dIgA, the receptor accumulated in recycling basolateral endosomes. In coculture, however, dIgA transcytosis by A664 receptor was normal. Thus, entry of receptor into the transcytotic pathway requires Ser-664 phosphorylation only in the absence of dIgA.

  • Short Title: Transcytosis of the polymeric Ig receptor requires phosphorylation of serine 664 in the absence but not the presence of dimeric IgA
  • Date: 30-07-1993
  • Journal: Cell
  • Volume: 74
  • Issue: 2
  • Pages: 245-255
  • Publisher: Cell Press
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence Animals Base Sequence Biological Transport *Cell Polarity Cells, Cultured Culture Techniques Dogs Epithelium/immunology/metabolism Hybridomas Immunoglobulin A/*metabolism Kidney/cytology/metabolism Molecular Sequence Data Mucous Membrane/immunology/metabolism Mutation Peptide Fragments/chemistry Phosphorylation Phosphoserine/analysis Receptors, Immunologic/genetics/*metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Secretory Component/genetics/*metabolism Transfection

Staff

Professor Robert Hirt
Professor of Evolutionary Parasitology