Publication:

Basal chloride currents in murine airway epithelial cells: modulation by CFTR (1998)

Author(s): R. Tarran;M. A. Gray;M. J. Evans;W. H. Colledge;R. Ratcliff;B. E. Argent

  • : Basal chloride currents in murine airway epithelial cells: modulation by CFTR

Abstract: We have isolated ciliated respiratory cells from the nasal epithelium of wild-type and cystic fibrosis (CF) null mice and used the patch-clamp technique to investigate their basal conductances. Current-clamp experiments on unstimulated cells indicated the presence of K+ and Cl[-] conductances and, under certain conditions, a small Na+ conductance. Voltage-clamp experiments revealed three distinct Cl[-] conductances. Itv-indep was time and voltage independent with a linear current-voltage (I-V) plot; Iv-act exhibited activation at potentials greater than {+/-}50 mV, giving an S-shaped I-V plot; and Ihyp-act was activated by hyperpolarizing potentials and had an inwardly rectified I-V plot. The current density sequence was Ihyp-act = Iv-act [>>] Itv-indep. These conductances had Cl[-]-to-N-methyl-D-glucamine cation permeability ratios of between 2.8 and 10.3 and were unaffected by tamoxifen, flufenamate, glibenclamide, DIDS, and 5-nitro-2-(3-phenylpropylamino) benzoic acid but were inhibited by Zn2+ and Gd3+. Itv-indep and Iv-act were present in wild-type and CF cells at equal density and frequency. However, Ihyp-act was detected in only 3% of CF cells compared with 26% of wild-type cells, suggesting that this conductance may be modulated by cystic fibrosis transmembrane conductance regulator (CFTR).

  • Short Title: Basal chloride currents in murine airway epithelial cells: modulation by CFTR
  • Alternate Journal: Am J Physiol Cell Physiol
  • Date: April 1, 1998
  • Journal: Am J Physiol Cell Physiol
  • Volume: 274
  • Issue: 4
  • Pages: C904-913
  • Publication type: Article
  • Bibliographic status: Published
Staff

Professor Barry Argent
Professor of Cellular Physiology