Publication:

A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription (1991)

Author(s): Lydall D, Ammerer G, Nasmyth K.

  • : A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription

Abstract: In the yeast Saccharomyces cerevisiae cell cycle-regulated SW15 transcription is essential for ensuring that mother and not daughter cells switch mating type. We have identified a 55-bp promoter sequence that appears to be responsible for restricting transcription to the late S, G2, and M phases of the cell cycle. Two proteins, MCM1, a transcription factor described previously, and SFF (SWI five factor, a newly identified factor) bind this sequence in vitro. MCM1 binds the DNA tightly on its own, but SFF will only bind as part of a ternary complex with MCM1. We observe a strong correlation between the ability of mutated SWI5 promoter sequences to form a ternary MCM1-SFF-containing complex in vitro and to activate transcription in vivo, which suggests that efficient transcription requires that both proteins bind DNA. Through its interactions with cell type-specific coactivators and corepressors, MCM1 controls cell type-specific expression of pheromone and receptor genes. By analogy, we propose that SFF enables MCM1 to function as a part of a cell cycle-regulated transcription complex.

Notes: 0890-9369 Journal Article

  • Short Title: A new role for MCM1 in yeast: cell cycle regulation of SW15 transcription
  • Date: Dec
  • Journal: Genes and Development
  • Volume: 5
  • Issue: 12B
  • Pages: 2405-19
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Base Composition Base Sequence *Cell Cycle Fungal Proteins/chemistry/genetics/*metabolism *Gene Expression Regulation, Fungal MCM1 Protein Molecular Sequence Data Promoter Regions (Genetics) Protein Binding RNA, Fungal/metabolism Saccharomyces cerevisiae/*genetics Support, Non-U.S. Gov't Transcription Factors/genetics/*physiology *Transcription, Genetic

Staff

Professor David Lydall
Professor of Genome Stability