Publication:

T-STAR/ETOILE: a novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis (1999)

Author(s): Venables JP, Vernet C, Chew SL, Elliott DJ, Cowmeadow RB, Wu J, Cooke HJ, Artzt K, Eperon IC

    Abstract: RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells. Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function. The mouse homologue was also cloned and designated etoile. It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8. T-STAR/etoile is expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/etoile and SAM68 is regulated during meiosis. Transfection of T-STAR/etoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the peri-nucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ETOILE and SAM68.

      • Date: 06-06-1999
      • Journal: Human Molecular Genetics
      • Volume: 8
      • Issue: 6
      • Pages: 959-969
      • Publisher: Oxford University Press
      • Publication type: Article
      • Bibliographic status: Published
      Staff

      Professor David Elliott
      Professor of Genetics