Publication:

Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme (2001)

Author(s): Firbank SJ; Rogers MS; Wilmot CM; Dooley DM; Halcrow MA; Knowles PF; McPherson MJ; Phillips SE

  • : Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme

Abstract: Galactose oxidase (EC ) is a monomeric enzyme that contains a single copper ion and catalyses the stereospecific oxidation of primary alcohols to their corresponding aldehydes. The protein contains an unusual covalent thioether bond between a tyrosine, which acts as a radical center during the two-electron reaction, and a cysteine. The enzyme is produced in a precursor form lacking the thioether bond and also possessing an additional 17-aa pro-sequence at the N terminus. Previous work has shown that the aerobic addition of Cu(2+) to the precursor is sufficient to generate fully processed mature enzyme. The structure of the precursor protein has been determined to 1.4 A, revealing the location of the pro-sequence and identifying structural differences between the precursor and the mature protein. Structural alignment of the precursor and mature forms of galactose oxidase shows that five regions of main chain and some key residues of the active site differ significantly between the two forms. The precursor structure provides a starting point for modeling the chemistry of thioether bond formation and pro-sequence cleavage.

Notes: GM 27659/GM/United States NIGMS

Journal Article

Research Support, Non-U.S. Gov't

Research Support, U.S. Gov't, P.H.S.

United States

  • Short Title: Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme
  • Alternate Journal: Proceedings of the National Academy of Sciences of the United States of America
  • Date: Nov 6
  • Journal: Proc Natl Acad Sci U S A
  • Volume: 98
  • Issue: 23
  • Pages: 12932-7
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence

Binding Sites

Crystallography, X-Ray

Enzyme Precursors/*chemistry

Galactose Oxidase/*chemistry

Molecular Sequence Data

Protein Conformation

Protein Processing, Post-Translational

Staff

Dr Susan Firbank

  • Telephone: +44 (0) 191 208 7369