Flow Cytometry; Colour Compensation - Short Course
At a Glance
Course dates: 15th & 16th December
Location: Flow Cytometry Facility, Centre for Life, Newcastle University
CPD Hours: 100 (of which 16 hours face to face)
Post Graduate Credits: 10 Masters Level
Course fees: £400
Course Contact: Ian Dimmick (ian.dimmick@ncl.ac.uk)
Module information
This module provides an understanding of the principles, concepts and technology of flow cytometry and the various functions that these technologies can fulfil in a clinical and research setting.
Students will begin by getting a thorough theoretical grounding and then move on to supervised hands-on experience of the instruments and experiments – including both basic and advanced multi-coloured experiments.
At the end of the module, students will be able to:
- set-up and run experiments on a broad range of flow cytometers
- assess the appropriate nature of multicolour experiments to be used on an individual instrument
- manipulate the 3 main systems of flow cytometry; optics, electronics, fluidics
- set-up flow cytometer for multi colour experiments
- manipulate and display results in various formats; 2D, 3D, histogram etc
- critically appraise results from flow cytometry experiments
- explain and demonstrate results / techniques to colleagues
Group numbers are kept low to enable individual teaching and supervision and it is envisioned that following this module participants will be fully equipped to use flow cytometry in their workplaces with a high degree of confidence and competency.
Key topics
- Theory for optimal PMT settings
- The use of Isotope controls and alternate strategies
- The use of FMO controls
- Instrument configurations and selection of complimentary fluorochromes & dyes
- Spectral overlap differences within product ranges
- How to view and control colour compensation
- A practical demonstration
- Instrument set up & QC
- Voltage setting
- Fluorochrome choice
- Sample experiments from 2 to 8 colour compensation
Assessment
Practical test and presentation to colleagues (50%), multiple choice questions (25%), written worksheets.
Entry requirements
Academic: The entry requirements will normally be a second-class Honours degree, or an international equivalent. Other equivalent qualifications and/or relevant industrial experience will be considered for entry.
Language: Applicants whose first language is not English require IELTS 6.5, TOEFL 90 (Internet-based) or 577 (paper-based), or equivalent.
How to apply
For admissions or general enquiries please contact the module leader:
Mr Ian Dimmick
Flow Cytometry Core Facility Manager
Newcastle upon Tyne University
Faculty of Medical Sciences
Bioscience Centre, International Centre for Life
Newcastle Upon Tyne, NE1 3BZ
Tel: +44 (0)191 241 8831
Email: ian.dimmick@ncl.ac.uk
Find out about the Flow Cytometry Facility
