Newcastle University

Introduction

The Dean of Postgraduate Studies and Director of the Graduate School has the primary role of leading the implementation of the Faculty's postgraduate strategy and quality assurance of the postgraduate research and teaching programmes. The Dean is assisted by a Graduate School Executive, including the Directors of PhD Studies, MD Studies, Postgraduate Taught Studies and International Postgraduate Studies and the Faculty Postgraduate Tutor, and by the Graduate School Committee comprising the Executive plus representative from each research institute and the degree programme directors for the postgraduate taught programmes.

Research Interests

Information about my research interests can be found on the Institute for Cell and Molecular Biosciences website.

• Howard A, Hirst BH. The glycine transporter GLYT1 in human intestine: expression and function. Biological and Pharmaceutical Bulletin 2011, 34(6), 784-788.
• Howard A, Tahir I, Javed S, Waring SM, Ford D, Hirst BH. Glycine transporter GLYT1 is essential for glycine-mediated protection human intestinal epithelial cells against oxidative damage. Journal of Physiology 2010, 588(6), 995–1009.
• McLure S, Willis M, Fox A, Newton J, Hirst B. Postgraduate opportunities in research at NEAS. Journal of Paramedic Practice 2010, 2(5), 230-232.
• Smith WD, Pointon JA, Abbot E, Kang HJ, Baker EN, Hirst BH, Wilson JA, Banfield MJ, Kehoe MA. Roles of Minor Pilin Subunits Spy0125 and Spy0130 in the Serotype M1 Streptococcus pyogenes Strain SF370. Journal of Bacteriology 2010,192 18 4651-4659.
• Hirst BH, Howard A, Javed S. [Meeting Abstract] Protection of intestinal cells against oxidative stress: A role for GLYT1-mediated transport. Journal of Physiological Sciences 2009,59 s1 247.
• Javed S, Howard A, Hirst B. Protection of intestinal cells against reactive oxygen species (ROS) by amino acids. In: Mutagenesis: 31st Annual Meeting of the United Kingdom Environmental Mutagen Society. 2009, University of Northumbria, Newcastle upon Tyne, UK: Oxford University Press.
• Fearn RA, Hirst BH. Predicting oral drug absorption and hepatobiliary clearance: Human intestinal and hepatic in vitro cell models. Environmental Toxicology and Pharmacology 2006, 21(2), 168-178.
• Nile CJ, Townes CL, Hirst BH, Hall J. The novel avian protein, AWAK, contains multiple domains with homology to protease inhibitory modules. Molecular Immunology 2006, 43(4), 388-394.
• Prime-Chapman H, Moore V, Hirst BH. Antibiotic exposure does not influence MRP2 functional expression in Caco-2 cells. Journal of Drug Targeting 2005, 13(1), 1-6.
• Siou GPS, Smith W, Kehoe MA, Hirst BH, Wilson JA. Do streptococcal fimbriae play a role in bacterial tonsillitis?. Annals of the Royal College of Surgeons of England 2005. , 87(6), 482-482.
• Foster N, Hirst BH. Exploiting receptor biology for oral vaccination with biodegradable particulates. Advanced Drug Delivery Reviews 2005. , 57(3), 431-450.
• Prime-Chapman HM, Fearn RA, Cooper AE, Moore V, Hirst BH. Differential multidrug resistance-associated protein 1 through 6 isoform expression and function in human intestinal epithelial Caco-2 cells. Journal of Pharmacology and Experimental Therapeutics 2004,311 2 476-484.
• Nile CJ, Townes CL, Michailidis G, Hirst BH, Hall J. Identification of chicken lysozyme g2 and its expression in the intestine. Cellular and Molecular Life Sciences 2004, 61(21), 2760-2766.
• Howard A, Goodlad RA, Walters JRF, Ford D, Hirst BH. Increased expression of specific intestinal amino acid and peptide transporter mRNA in rats fed by TPN is reversed by GLP-2. Journal of Nutrition 2004,134 11 2957-2964.
• Jepson MA, Clark MA, Hirst BH. M cell targeting by lectins: A strategy for mucosal vaccination and drug delivery. Advanced Drug Delivery Reviews 2004. ,56 4 511-525.
• Chan LMS, Cooper AE, Dudley ALJ, Ford D, Hirst BH. P-glycoprotein potentiates CYP3A4-mediated drug disappearance during Caco-2 intestinal secretory detoxification. Journal of Drug Targeting 2004,12 7 405-413.
• Hirst BH. Secretin and the exposition of hormonal control. Journal of Physiology 2004. ,560 2 339-339.
• Chan LMS, Lowes S, Hirst BH. The ABCs of drug transport in intestine and liver: Efflux proteins limiting drug absorption and bioavailability. European Journal of Pharmaceutical Sciences 2004. ,21 1 25-51.
• Aldridge PD, Gray MA, Hirst BH, Khan CMA. Who's talking to whom? Epithelial-bacterial pathogen interactions. In: International Symposium on Epithelial-Bacterial Pathogen Interactions. 2004, Newcastle upon Tyne, UK: Molecular Microbiology: Wiley-Blackwell.
• Jepson MA, Simmons NL, O'Hagan DT, Hirst BH. Comparison of poly(DL-lactide-co-glycolide) and polystyrene microsphere targeting to intestinal M cells. Journal of Drug Targeting 2003,11 5 269-272.
• Ford D, Howard A, Hirst BH. Expression of the peptide transporter hPepT1 in human colon: A potential route for colonic protein nitrogen and drug absorption. Histochemistry and Cell Biology 2003,119 1 37-43.
• Wilson V, Howard A, Ford D, Hirst BH. EGF enhanced transcription of the amino acid transporter hATB(0) in COS-7 cells. In: Journal of Physiology: Conference of the Physiological Society. 2002, Liverpool: Wiley-Blackwell Publishing Ltd.
• Howard A, Goodlad RA, Ford D, Walters JRF, Hirst BH. Glucagon-like peptide 2 (GLP-2) abrogates the effect of total parenteral nutrition (TPN) on intestinal amino acid and peptide transporters in rats. In: Journal of Physiology: Conference of the Physiological Society. 2002, Liverpool: Wiley-Blackwell Publishing Ltd.
• Hirst BH. K+ recycling and gastric acid secretion. Journal of Physiology 2002, 540(1), 1.
• Reuss L, Hirst BH. Water transport controversies - An overview. Journal of Physiology 2002, 542(1), 1-2.
• Clark MA, Jepson MA, Hirst BH. Exploiting M cells for drug and vaccine delivery. Advanced Drug Delivery Reviews 2001, 50(1-2), 81-106.
• Ann Clark M, Blair H, Liang L, Brey RN, Brayden D, Hirst BH. Targeting polymerised liposome vaccine carriers to intestinal M cells. Vaccine 2001, 20(1-2), 208-217.
• Howard A, Jordinson M, Goodlad RA, Walters JR, Ford D, Hirst BH. Changes in expression of intestinal peptide and amino acid transporters in rats fed by total parenteral nutrition. In: GASTROENTEROLOGY. 2000.
• Clark MA, Wilson C, Sama A, Wilson JA, Hirst BH. Differential cytokeratin and glycoconjugate expression by the surface and crypt epithelia of human palatine tonsils. Histochemistry and Cell Biology 2000, 114(4), 311-321.
• Clark MA, Hirst BH, Jepson MA. Lectin-mediated mucosal delivery of drugs and microparticles. Advanced Drug Delivery Reviews 2000, 43(2-3), 207-223.
• Chan L, Dudley A, Ford D, Hirst BH. MDR1- and MRP2-mediated secretion of rhodamine dyes. In: JOURNAL OF PHYSIOLOGY-LONDON. 2000.
• Gray PA, Ford D, Hirst BH. Regulation of the amino acid transporter hEAAC1 in human intestinal Caco-2 cells. In: FASEB JOURNAL. 2000.
• Howard A, Gray PA, Ford D, Hirst BH. System ASC activity and expression of the amino acid transporter ASCT1 in human intestinal Caco-2 cells. In: JOURNAL OF PHYSIOLOGY-LONDON. 2000.
• Fontes CMGA, Ali S, Gilbert HJ, Hazlewood GP, Hirst BH, Hall J. Bacterial xylanase expression in mammalian cells and transgenic mice. Journal of Biotechnology 1999, 72(1-2), 95-101.
• Notarianni E, Hirst BH. Electronic sodium transport mediated by an amiloride-sensitive conductance in a procine trophectoderm cell line. Placenta 1999, 20, 149-154.
• Hirst BH. Parietal cell membrane trafficking. American Journal of Physiology: Cell Physiology 1999, 277, C359-C360.
• Matthews, C.J., McEwan, G.T.A., Redfern, C.P.F., Thomas, E.J., Hirst, B.H. Absorptive apical amiloride-sensitive Na+ conductance in human endometrial epithelium. Journal of Physiology 1998, 513(2), 443-452.
• K. A. Reed,M. A. Clark,T. A. Booth,C. J. Hueck,S. I. Miller,B. H. Hirst,M. A. Jepson. Cell-contact-stimulated formation of filamentous appendages by Salmonella typhimurium does not depend on the type III secretion system encoded by Salmonella pathogenicity island 1. Infect Immun 1998, 66(5), 2007-17.
• C. B. Collares-Buzato,M. A. Jepson,G. T. McEwan,B. H. Hirst,N. L. Simmons. Co-culture of two MDCK strains with distinct junctional protein expression: a model for intercellular junction rearrangement and cell sorting. Cell Tissue Res 1998, 291(2), 267-76.
• C. B. Collares-Buzato,M. A. Jepson,N. L. Simmons,B. H. Hirst. Increased tyrosine phosphorylation causes redistribution of adherens junction and tight junction proteins and perturbs paracellular barrier function in MDCK epithelia. Eur J Cell Biol 1998, 76(2), 85-92.
• M. A. Clark,B. H. Hirst,M. A. Jepson. Inoculum composition and Salmonella pathogenicity island 1 regulate M-cell invasion and epithelial destruction by Salmonella typhimurium. Infect Immun 1998, 66(2), 724-31.
• M. A. Clark,B. H. Hirst,M. A. Jepson. M-cell surface beta1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M cells. Infect Immun 1998, 66(3), 1237-43.
• Ford D, Thwaites D, Simmons N, Gilbert H, Hirst B. Substrate upregulation of the human small intestinal peptide transporter, hPepT1. Journal of Physiology 1998, 507(3), 697-706.
• C. L. Scudamore,M. A. Jepson,B. H. Hirst,H. R. Miller. The rat mucosal mast cell chymase, RMCP-II, alters epithelial cell monolayer permeability in association with altered distribution of the tight junction proteins ZO-1 and occludin. Eur J Cell Biol 1998, 75(4), 321-30.
• N. Foster,M. A. Clark,M. A. Jepson,B. H. Hirst. Ulex europaeus 1 lectin targets microspheres to mouse Peyer's patch M-cells in vivo. Vaccine 1998, 16(5), 536-41.
• S. Ali,C. M. Fontes,G. P. Hazlewood,B. H. Hirst,A. J. Clark,H. J. Gilbert,J. Hall. Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice. Gene 1997, 202(1-2), 203-8.
• S. Ali,J. Hall,G. P. Hazlewood,B. H. Hirst,H. J. Gilbert. A protein targeting signal that functions in polarized epithelial cells in vivo. Biochem J 1996, 315 ( Pt 3), 857-62.
• M. A. Clark,K. A. Reed,J. Lodge,J. Stephen,B. H. Hirst,M. A. Jepson. Invasion of murine intestinal M cells by Salmonella typhimurium inv mutants severely deficient for invasion of cultured cells. Infect Immun 1996, 64(10), 4363-8.
• K. A. Reed,T. A. Booth,B. H. Hirst,M. A. Jepson. Promotion of Salmonella typhimurium adherence and membrane ruffling in MDCK epithelia by staurosporine. FEMS Microbiol Lett 1996, 145(2), 233-8.
• M. A. Jepson,M. A. Clark,N. Foster,C. M. Mason,M. K. Bennett,N. L. Simmons,B. H. Hirst. Targeting to intestinal M cells. J Anat 1996, 189 ( Pt 3), 507-16.
• Thwaites DT, Cavet M, Hirst BH, Simmons NL. Angiotensin-converting enzyme (ACE) inhibitor transport in human intestinal epithelial (Caco-2) cells. British Journal of Pharmacology 1995, 114(5), 981-986.
• Thwaites DT, Armstrong G, Hirst BH, Simmons NL. D-Cycloserine transport in human intestinal epithelial (Caco-2) cells is mediated by a H⁺-coupled amino acid transporter. British Journal of Pharmacology 1995, 115(5), 761-766.
• Thwaites DT, McEwan GTA, Hirst BH, Simmons NL. H⁺-coupled alpha-methylaminoisobutyric acid transport in human intestinal Caco-2 cells. Biochimica et Biophysica Acta: Biomembranes 1995, 1234(1), 111-118.
• Thwaites DT, Hirst BH, Simmons NL. H⁺-coupled solute transport in cultured intestinal epithelia. In:Molecular and Cellular Mechanisms of H⁺ Transport . Heidelberg: Springer-Verlag, 1994, pp. 283-296.
• Thwaites DT, McEwan GTA, Brown CDA, Hirst BH, Simmons NL. L-Alanine absorption in human intestinal cells driven by the proton electrochemical gradient. Journal of Membrane Biology 1994, 140(2), 143-151.
• Thwaites DT, Hirst BH, Simmons NL. Substrate specificity of the di/tripeptide transporter in human intestinal epithelia (Caco-2): identification of substrates that undergo H⁺-coupled absorption. British Journal of Pharmacology 1994, 113(3), 1050-1056.
• Matthews CJ, Redfern CPF, Thomas EJ, Hirst BH. Bombesin and gastrin-releasing peptide stimulate electrogenic ion transport in cultured human endometrial epithelial cell layers. Experimental Physiology 1993, 78(5), 715-718.
• Matthews CJ, McEwan GTA, Redfern CPF, Thomas EJ, Hirst BH. Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium. Pflügers Archiv European Journal of Physiology 1993, 422(4), 401-403.
• Thwaites DT, Hirst BH, Simmons NL. Direct assessment of dipeptide/H⁺ symport in intact human intestinal (Caco-2) epithelium: a novel method utilising continuous intracellular pH measurement. Biochemical and Biophysical Research Communications 1993, 194(1), 432-438.
• Thwaites DT, McEwan GTA, Cook MJ, Hirst BH, Simmons NL. H⁺-coupled (Na⁺-independent) proline transport in human intestinal (Caco-2) epithelial cell monolayers. FEBS Letters 1993, 333(1-2), 78-82.
• Thwaites DT, Brown CDA, Hirst BH, Simmons NL. H⁺-coupled dipeptide (glycylsarcosine) transport across apical and basal borders of human intestinal Caco-2 cell monolayers display distinctive characteristics. Biochimica et Biophysica Acta: Biomembranes 1993, 1151(2), 237-245.
• Matthews CJ, Thomas EJ, Redfern CPF, Hirst BH. Ion transport by human endometrial epithelia in vitro. Human Reproduction 1993, 8(10), 1570-1575.
• Thwaites DT, McEwan GTA, Brown CDA, Hirst BH, Simmons NL. Na⁺-independent, H⁺-coupled transepithelial beta-alanine absorption by human intestinal Caco-2 cell monolayers. Journal of Biological Chemistry 1993, 268, 18438-18441.
• Thwaites DT, Hirst BH, Simmons NL. Passive transepithelial absorption of thyrotropin-releasing hormone (TRH) via a paracellular route in cultured intestinal and renal epithelial cell lines. Pharmaceutical Research 1993, 10(5), 674-681.
• Thwaites DT, Simmons NL, Hirst BH. Thyrotropin-releasing hormone (TRH) uptake in intestinal brush-border membrane vesicles: comparison with proton-coupled dipeptide and Na⁺-coupled glucose transport. Pharmaceutical Research 1993, 10(5), 667-673.
• Thwaites DT, McEwan GTA, Hirst BH, Simmons NL. Transepithelial dipeptide (glycylsarcosine) transport across epithelial monolayers of human Caco-2 cells is rheogenic. Pfluegers Archiv: European Journal of Physiology 1993, 425(1-2), 178-180.
• Thwaites DT, Brown CDA, Hirst BH, Simmons NL. Transepithelial glycylsarcosine transport in intestinal Caco-2 cells mediated by expression of H⁺-coupled carriers at both apical and basal membranes. Journal of Biological Chemistry 1993, 268(11), 7640-7642.
• Matthews CJ, Redfern CPF, Hirst BH, Thomas EJ. Characterization of human purified epithelial and stromal cells from endometrium and endometriosis in tissue culture. Fertility and Sterility 1992, 57(5), 990-997.
• MATTHEWS CJ, MCEWAN GTA, REDFERN CPF, THOMAS EJ, HIRST BH. ELECTRICAL-PROPERTIES OF PRIMARY MONOLAYER-CULTURES OF HUMAN ENDOMETRIAL GLANDULAR EPITHELIAL-CELLS. In: JOURNAL OF PHYSIOLOGY-LONDON. 1992.
• MATTHEWS CJ, REDFERN CPF, THOMAS EJ, HIRST BH. ELECTROLYTE TRANSPORT BY PRIMARY MONOLAYER-CULTURES OF HUMAN ENDOMETRIAL GLANDULAR EPITHELIAL-CELLS. In: JOURNAL OF PHYSIOLOGY-LONDON. 1992.
• Matthews CJ, Redfern CPF, Hirst BH, Thomas EJ. Epitopes for endometrial cells - Reply. Fertility and Sterility 1992. , 58(6), 1267-1268.
• MATTHEWS CJ, MCEWAN GTA, REDFERN CPF, THOMAS EJ, HIRST BH. ISOLATION, CULTURE AND FUNCTIONAL-ANALYSIS OF GLANDS FROM HUMAN ENDOMETRIUM. In: JOURNAL OF PHYSIOLOGY-LONDON. 1992.
• HIRST BH, COY DH. STRUCTURE-ACTIVITY STUDIES WITH SOMATOSTATIN - ROLE OF LYSINE IN POSITION-4 AND POSITION-9 FOR GASTRIC ACTIVITY. REGULATORY PEPTIDES 1984, 8(4), 267-271.
• HIRST BH, HOLLAND J. GASTRIC-MUCOSAL BLOOD-FLOW AND ACID-SECRETION IN THE CONSCIOUS CAT. In: JOURNAL OF PHYSIOLOGY-LONDON. 1981.
• GASCOIGNE AD, HIRST BH. PROSTAGLANDINS ALTER THE RELATIONSHIP BETWEEN GASTRIC HYDROGEN-ION CONCENTRATION AND FLOW - EVIDENCE FOR STIMULATION OF NON-PARIETAL SECRETION IN THE CAT. JOURNAL OF PHYSIOLOGY-LONDON 1981, 316(JUL), 427-438.
• Bell S, Howard A, Wilson JA, Abbot EL, Smith WD, Townes CL, Hirst BH, Hall J. Streptococcus pyogenes infection of tonsil explants is associated with a human B-defensin 1 response from control but not recurrent acute tonsillitis patients. Molecular Oral Microbiology 2012, 27(3), 160-171.
• O'Driscoll M, Cerosaletti KM, Girard PM, Dai Y, Stumm M, Kysela B, Hirsch B, Gennery A, Palmer SE, Seidel J, Gatti RA, Varon R, Oettinger MA, Neitzel H, Jeggo PA, Concannon P. DNA ligase IV mutations identified in patients exhibiting developmental delay and immunodeficiency. Molecular Cell 2001, 8(6), 1175-1185.
• Prime-Chapman HM, Moore V, Hirst BH. Expression of multidrug resistance-associated proteins 1-6 in human intestinal Caco-2 cells. In: Meeting of the Physiological Society. 2001, Sheffield, UK: Wiley-Blackwell Publishing Ltd.
• Christie GR, Ford D, Howard A, Clark M Ann, Hirst BH. Glycine supply to human enterocytes mediated by high-affinity basolateral GLYT1. Gastroenterology 2001, 120(2), 439-448.
• Chan L, Dudley A, Keating K, Cooper AE, Ford D, Hirst BH. Interactions of testosterone with cytochrome P450 3A4 and P-glycoprotein in human intestinal Caco-2 cells. In: Meeting of the Physiological Society. 2001, Sheffield, UK: Wiley-Blackwell Publishing Ltd.
• Howard A, Rugg-Gunn P, Goodlad RL, Walters JRF, Ford D, Hirst BH. Regional differences in small intestinal nutrient transporter transcript expression during total parenteral nutrition. In: FASEB Journal. 2001, Federation of American Societies for Experimental Biology.