Flow Cytometry - University of Newcastle Core Facility
The Flow cytometry core facility is currently split across two sites within the University campus to enable optimal access for cell sorting and analytical flow cytometry.
The two sites that house the shared facility are the Institute of Human Genetics, International centre for life and 3rd Floor William Leech building, Medical school .
Across the two sites we have a comprehensive suite of instrumentation which includes
Cell Sorting
The sorting facilities offer a 4 laser custom built Aria II , as well as a FACSDiva cell sorter
Multi parameter cell analysis
High end instrumentation includes
LSR II- 5 laser (2 Forward scatter detectors, 1 of which is PMT driven) with H.T.S.
LSR II- 3 laser
FACSCanto II 3 laser
Routine analysis instrumentation
FACSCalibur
FACSCan (x3)
Support instrumentation
Beckman Coulter Vi Cell , cell and viability counter.
Becton Dickinson Lyse wash assistant (x2)
Training and advice is supplied by 3 dedicated staff who are happy to advise on experimental design and the optimal use of appropriate instrumentation .
The department also runs an in depth 2 day colour compensation course and a yearly one day flow cytometry meeting, both of these courses attract local and European delegate interest.
For any further details regarding flow cytometry please contact:
Ian.Dimmick@ncl.ac.uk - Manager
R.Stewart@ncl.ac.uk - Flow cytometry specialist
I.J.Harvey@ncl.ac.uk - Flow cytometry specialist
