Publication:

Glutathione transferase pi its minimal promoter and downstream cis- acting element (1991)

Author(s): Xia CL, Cowell IG, Dixon KH, Pemble SE, Ketterer B, Taylor JB

    Abstract: Fragments of the human glutathione S-transferase pi gene and 15 kb of its 5' flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -58 and -65 (Cowell et al. 1988. Biochem. J. 255, 79-83), is essential for basal level promoter activity. We have also identified a positive cis-acting DNA element between nucleotides +8 and +72 which seems to be part of the promoter. No other regulatory activity was identified within the 17 kb analyzed

      • Date: 15-04-1991
      • Journal: Biochemical and Biophysical Research Communications
      • Volume: 176
      • Issue: 1
      • Pages: 233-240
      • Publisher: Academic Press
      • Publication type: Article
      • Bibliographic status: Published

      Keywords: Animal AP1 Base Sequence binding site Binding Sites Bladder Neoplasms Breast Neoplasms c-jun Cancer Carcinoma,Hepatocellular Cell line Chloramphenicol O-Acetyltransferase cis acting element Comparative Study dna DNA binding dna binding protein DNA-Binding Proteins enzymology Exons female gene genetics glutathione S-transferase glutathione transferase Hela Cells human human cell Introns Isoenzymes kinase Liver Neoplasms MCF7 metabolism Molecular Sequence Data Nucleotides oligonucleotide Oligonucleotide Probes plasmid Plasmids promoter Promoter Regions (Genetics) protein Protein-Tyrosine Kinase Proteins proto oncogene Proto-Oncogene Proteins Rats Recombinant Fusion Proteins reporter gene Sequence Homology,Nucleic Acid transcription transcription factor transcription factors transfection United States Proto-Oncogene Proteins c-jun nucleotide

      Staff

      Dr Ian Cowell
      Team Scientist