Quantitative Proteomic Analysis
In recent years there has been considerable interest in the application of proteomics to changes in global proteome expression and how such changes relate to variations at the cellular level.
Potential applications include biomarker discovery, understanding disease processes and other medical applications (Clinical Proteomics) as well as performing comparisons of different biological states such as mutant versus wild-type or growth under differing conditions of stress or nutrients.
The technologies that have been applied to this quantitation problem include antibody-based arrays, two-dimensional gel electrophoresis (2D PAGE) and LC-MS or MS/MS-based mass spectrometry.
Many researches now prefer the MS/MS-based approach because it offers high throughput & highly sensitive analytical capabilities, relatively large dynamic range for detection and a number methods for quantitative measurement.
To date there have been a number of methodologies proposed towards making proteome-wide changes in protein expression a reliable & routine analysis procedure including:
- Metabolic Labeling (e.g. SILAC)
- Chemical Tagging (e.g. iTRAQ)
- Enzymatic Labeling (e.g. 16O/18O)
- Labeled Reference Standard Peptide(s) (e.g. AQUA)
- Label-Free Approach (e.g. Spectral Counting)
Pinnacle offers several of the above as options in our quantitative proteomics packages. Costs can vary depending on the number of sample conditions under consideration, the choice of methodology and the requirement for statistically confident data sets (replicates).
If you already have a proposal outline, or are considering quantitative proteomics for a future research project, you are welcome to contact us and we'll be happy to advise on experimental design and costs for your project grant application.
If you would like to discuss your proposal or need advice about pricing feel free to contact the unit.
Note: Details of our two-dimensional gel electrophoresis (2D PAGE) service can be found here.
