Publication:
Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly (2011)
Author(s): Faulds-Pain A, Birchall C, Aldridge C, Smith WD, Grimaldi G, Nakamura S, Miyata T, Gray J, Li GL, Tang JX, Namba K, Minamino T, Aldridge PD
Abstract: Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in Delta fljK Delta fljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament.
- Date: 25-03-2011
- Journal: Journal of Bacteriology
- Volume: 193
- Issue: 11
- Pages: 2695-2707
- Publisher: American Society for Microbiology
- Publication type: Article
- Bibliographic status: Published
- URL: http://dx.doi.org/10.1128/JB.01172-10
- DOI: 10.1128/JB.01172-10
- ISSN (electronic): 1098-5530
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Christine Aldridge
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Dr Phillip Aldridge
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