Cells
Publication:

Flagellin Redundancy in Caulobacter crescentus and Its Implications for Flagellar Filament Assembly (2011)

Author(s): Faulds-Pain A, Birchall C, Aldridge C, Smith WD, Grimaldi G, Nakamura S, Miyata T, Gray J, Li GL, Tang JX, Namba K, Minamino T, Aldridge PD

    Abstract: Bacterial flagella play key roles in surface attachment and host-bacterial interactions as well as driving motility. Here, we have investigated the ability of Caulobacter crescentus to assemble its flagellar filament from six flagellins: FljJ, FljK, FljL, FljM, FljN, and FljO. Flagellin gene deletion combinations exhibited a range of phenotypes from no motility or impaired motility to full motility. Characterization of the mutant collection showed the following: (i) that there is no strict requirement for any one of the six flagellins to assemble a filament; (ii) that there is a correlation between slower swimming speeds and shorter filament lengths in Delta fljK Delta fljM mutants; (iii) that the flagellins FljM to FljO are less stable than FljJ to FljL; and (iv) that the flagellins FljK, FljL, FljM, FljN, and FljO alone are able to assemble a filament.

      • Date: 25-03-2011
      • Journal: Journal of Bacteriology
      • Volume: 193
      • Issue: 11
      • Pages: 2695-2707
      • Publisher: American Society for Microbiology
      • Publication type: Article
      • Bibliographic status: Published
      Staff

      Christine Aldridge
      Research Associate

      Dr Phillip Aldridge
      Senior Lecturer