Identification of two human phosphoproteins (dividin and IEF 59dl) that are first detected late in G1 near the G1/S transition border of the cell cycle (1987)

Author(s): Nielsen SU, Celis A, Ratz GP, Celis JE

    Abstract: Two-dimensional gel electrophoretic analysis (NEPHGE, IEF) of the [32P]-orthophosphate-labeled proteins synthesized throughout the cell cycle of transformed human amnion cells (AMA) revealed two phosphoproteins (dividin, Mr = 54,000, pl = 8.4; IEF 59dl, Mr = 27,000, pl = 5.7) that are present mainly in S-phase cells. These proteins are first detected at the end of G1, near the G1/S transition border, and their levels reach a maximum late in S-phase. Together with the previously identified nuclear protein cyclin, these phosphoproteins are likely candidates for proteins that may play a role in the regulation of the onset of DNA synthesis and cell division.

      • Date: 01-01-1987
      • Journal: Leukemia
      • Volume: 1
      • Issue: 1
      • Pages: 69-77
      • Publisher: Nature Publishing Group
      • Publication type: Article
      • Bibliographic status: Published

      Keywords: Amnion

      *Cell Cycle

      Cell Line, Transformed

      Electrophoresis, Polyacrylamide Gel



      Isoelectric Point


      Molecular Weight



      Dr Soren Nielsen
      Research Associate