Publication:
Identification of two human phosphoproteins (dividin and IEF 59dl) that are first detected late in G1 near the G1/S transition border of the cell cycle (1987)
Author(s): Nielsen SU, Celis A, Ratz GP, Celis JE
Abstract: Two-dimensional gel electrophoretic analysis (NEPHGE, IEF) of the [32P]-orthophosphate-labeled proteins synthesized throughout the cell cycle of transformed human amnion cells (AMA) revealed two phosphoproteins (dividin, Mr = 54,000, pl = 8.4; IEF 59dl, Mr = 27,000, pl = 5.7) that are present mainly in S-phase cells. These proteins are first detected at the end of G1, near the G1/S transition border, and their levels reach a maximum late in S-phase. Together with the previously identified nuclear protein cyclin, these phosphoproteins are likely candidates for proteins that may play a role in the regulation of the onset of DNA synthesis and cell division.
- Date: 01-01-1987
- Journal: Leukemia
- Volume: 1
- Issue: 1
- Pages: 69-77
- Publisher: Nature Publishing Group
- Publication type: Article
- Bibliographic status: Published
- Author Address: Department of Medical Biochemistry, Aarhus University, Denmark.
- ISSN (electronic): 1476-5551
Keywords: Amnion
*Cell Cycle
Cell Line, Transformed
Electrophoresis, Polyacrylamide Gel
Humans
Interphase
Isoelectric Point
Mitosis
Molecular Weight
Phosphoproteins/*metabolism
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Dr Soren Nielsen
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