Cells
Publication:

Cloning and sequencing of the cDNA for a 13th different subunit (IHQ) of beef heart cytochrome c oxidase (1989)

Author(s): R. Lightowlers;S. Takamiya;R. Wessling;M. Lindorfer;R. A. Capaldi

  • : Cloning and sequencing of the cDNA for a 13th different subunit (IHQ) of beef heart cytochrome c oxidase

Abstract: The mammalian cytochrome c oxidase is a complex of 13 different subunits. We present the full amino acid sequence of the one remaining uncharacterized subunit, subunit IHQ in the nomenclature used here, VIIb in the numbering system of Kadenbach and colleagues (e.g. Kadenbach, B., and Merle, P. (1981) FEBS Lett. 135, 1-11). A partial protein sequence was obtained from the purified subunit isolated by gel filtration procedures. This information was used to synthesize an oligonucleotide probe which was then used to isolate a cDNA clone encoding the subunit. This cDNA for subunit IHQ is 480 base pairs long and encodes a polypeptide which is either 83 or 88 amino acids long, including an N-terminal leader sequence of either 27 or 32 residues. The molecular weight of the mature subunit IHQ is 6350 based on the amino acid sequence deduced from the gene. The leader sequence is typical of other mitochondrial target sequences in having several positively charged residues but no negatively charged side chains.

Notes: 0021-9258 Journal Article

  • Short Title: Cloning and sequencing of the cDNA for a 13th different subunit (IHQ) of beef heart cytochrome c oxidase
  • Date: Oct 5
  • Journal: J Biol Chem
  • Volume: 264
  • Issue: 28
  • Pages: 16858-61
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence Animals Base Sequence Cattle *Cloning, Molecular DNA/*genetics/isolation & purification Electron Transport Complex IV/*genetics/isolation & purification Macromolecular Substances Molecular Sequence Data Molecular Weight Myocardium/*enzymology Oligonucleotide Probes Research Support, U.S. Gov't, P.H.S. Terminology

Staff

Professor Robert Lightowlers
Director, ICaMB and Professor of Molecular Neuroscience