Cells
Publication:

Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family (1991)

Author(s): R. D. Carrero-Valenzuela;F. Quan;R. Lightowlers;N. G. Kennaway;M. Litt;M. Forte

  • : Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family

Abstract: We report the isolation and sequence of a human heart cDNA coding for cytochrome c oxidase (COX) subunit VIb (COX VIb). This cDNA extends 50 bp upstream from the region coding for the mature peptide. By Northern analysis, a single transcript of approx. 550 nucleotides (nt) has been identified in six human tissues. Southern analysis of human genomic DNA demonstrates the presence of multiple loci that show high homology to the cDNA. These loci cosegregate with either five or six different human chromosomes in human-rodent somatic cell hybrids. Using the COX6b cDNA, genomic sequences representing two of these loci have been isolated and characterized. The nt sequence analysis suggests that both loci represent COX6b pseudogenes.

Notes: 0378-1119 Journal Article

  • Short Title: Human cytochrome c oxidase subunit VIb: characterization and mapping of a multigene family
  • Date: Jun 30
  • Journal: Gene
  • Volume: 102
  • Issue: 2
  • Pages: 229-36
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Dna Electron Transport Complex IV/*genetics/metabolism Humans Molecular Sequence Data *Multigene Family Myocardium/*enzymology RNA, Messenger/metabolism Research Support, Non-U.S. Gov't Restriction Mapping Sequence Homology, Nucleic Acid

Staff

Professor Robert Lightowlers
Director, ICaMB and Professor of Molecular Neuroscience