Dr Heath Murray
Royal Society Research Fellow
- Email: email@example.com
- Telephone: +44 (0) 191 208 3233
- Fax: +44 0 (191) 208 7424
- Address: The Centre for Bacterial Cell Biology
Medical Science New Building
Newcastle upon Tyne
Royal Society University Research Fellow (2009-2017)
Postdoctoral Fellow (2004-2009) - Professor Jeff Errington, FRS FMedSci
Graduate Fellow (1998-2003) - Professor Richard Gourse, PhD
Undergraduate Scholar (1994-1998) - Associate Professor Robert Simons, PhD
Ph.D. Genetics (University of Wisconsin-Madison, USA)
B.S. Microbiology & Molecular Genetics (University of California-Los Angeles, USA)
HONORS AND AWARDS
Royal Society University Research Fellow (2009-2017)
Human Frontier Science Program Postdoctoral Fellowship (2005-2007)
European Molecular Biology Organization Postdoctoral Fellowship (2004)
USA National Institutes of Health Postdoctoral Fellowship (2004; declined)
UW-Madison Genetics Department Outstanding Graduate Student Award (2003)
National Institutes of Health Genetics Training Fellowship (1998-2001)
Howard Hughes Undergraduate Research Fellowship (1996, 1997)
University of California Regents Scholarship (1994-1998)
Bacterial DNA Replication Initiation
The Murray Lab studies bacterial DNA replication initiation in the model organism Bacillus subtilis. Using a combination of genetics, cell biology, molecular biology, and biochemistry, we are investigating the structure of the bacterial chromosome origin and the activity of replication initiation proteins required to unwind the DNA duplex and to load the replicative helicases. One of the key tools we have developed is an inducible artificial chromosome origin that allows us to bypass normally essential endogenous replication initiation factors. We are keen to explore the utility of more sophisticated synthetic replication systems, both for fundamental understanding of DNA replication and for exploitation in biotechnology. We are also utilizing our expertise to search for novel antibiotics that target DNA replication proteins.
Christopher Blower - UG student
Omar Harran - PhD student
Stuart Middlemiss - Masters student
Tom Richardson - Postdoc
Daniel Stevens - PhD student
Several postdoc positions to work on a Wellcome Trust funded project entitled "Investigating the physiological DNA replication initiation reaction" are available. Interested individuals should have a strong background in bacterial genetics, molecular biological, biochemistry, and/or high-throughput screening, and should have a demonstrated record of high productivity in peer-reviewed literature. Interested candidates should send a CV, along with two reference letters, directly to firstname.lastname@example.org.
BMG2056 - DNA Replication & Recombination
CMB3000 - Undergraduate Research project
MIC2026 - Microbial Cell Biology
MIC2027 - Extended Scientific Essay
MMB8016 - Control of DNA Replication in Bacteria
- LeGall A, Cattoni DI, Guilhas B, Mathieu-Demazière C, Oudjedi L, Fiche JB, Rech J, Abrahamsson S, Murray H, Bouet JY, Nollmann M. Bacterial partition complexes segregate within the volume of the nucleoid. Nature Communications 2016, 7, 12107.
- Murray H. Connecting chromosome replication with cell growth in bacteria. Current Opinion in Microbiology 2016, 34, 13-17.
- Du WL, Dubarry N, Passot F, Kamgoue A, Murray H, Lane D, Pasta F. Orderly replication and segregation of the four replicons of Burkholderia cenocepacia J2315. PLOS Genetics 2016, 12(7), e1006172.
- Koh A, Murray H. Probing chromosome dynamics in Bacillus subtilis. In: Methods in Molecular Biology. Springer, 2016, pp.91-108.
- Richardson TT, Harran O, Murray H. The bacterial DnaA-trio replication origin element specifies ssDNA initiator binding. Nature 2016, 534(7607), 412-416.
- Marbouty M, LeGall A, Cattoni DI, Cournac A, Koh A, Fiche JB, Mozziconacci J, Murray H, Koszul R, Nollmann M. Condensin- and replication-mediated bacterial chromosome folding and origin condensation revealed by Hi-C and super-resolution imaging. Molecular Cell 2015, 59(4), 588-602.
- Murray H, Koh A. Multiple Regulatory Systems Coordinate DNA Replication with Cell Growth in Bacillus subtilis. PLoS Genetics 2014, 10(10), e1004731.
- Jameson KH, Rostami N, Fogg MJ, Turkenburg JP, Grahl A, Murray H, Wilkinson AJ. Structure and interactions of the Bacillus subtilis sporulation inhibitor of DNA replication, SirA, with domain I of DnaA. Molecular Microbiology 2014, 93(5), 975-991.
- Boonstra M, de Jong IG, Scholefield G, Murray H, Kuipers OP, Veening JW. Spo0A regulates chromosome copy number during sporulation by directly binding to the origin of replication in Bacillus subtilis. Molecular Microbiology 2013, 87(4), 925-938.
- Scholefield G, Murray H. YabA and DnaD inhibit helix assembly of the DNA replication initiation protein DnaA. Molecular Microbiology 2013, 90(1), 147-159.
- Scholefield G, Errington J, Murray H. Soj/ParA stalls DNA replication by inhibiting helix formation of the initiator protein DnaA. EMBO Journal 2012, 31(6), 1542-1555.
- Scholefield G, Veening JW, Murray H. DnaA and ORC: more than DNA replication initiators. Trends in Cell Biology 2011, 21(3), 188-194.
- Scholefield G, Whiting R, Errington J, Murray H. Spo0J regulates the oligomeric state of Soj to trigger its switch from an activator to an inhibitor of DNA replication initiation. Molecular Microbiology 2011, 79(4), 1089-1100.
- Engl C, Jovanovic G, Lloyd LJ, Murray H, Spitaler M, Ying LM, Errington J, Buck M. In vivo localizations of membrane stress controllers Escherichia coli. Molecular Microbiology 2009, 73(3), 382-396.
- Veening JW, Murray H, Errington J. A mechanism for cell cycle regulation of sporulation initiation in Bacillus subtilis. Genes & Development 2009, 23(16), 1959-1970.
- Murray HD, Errington J. Dynamic Control of the DNA Replication Initiation Protein DnaA by Soj/ParA. Cell 2008, 135(1), 74-84.
- Murray H, Ferreira H, Errington J. The bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites. Molecular Microbiology 2006, 61(5), 1352-1361.
- Errington J, Murray H, Wu LJ. Diversity and redundancy in bacterial chromosome segregation mechanisms. Philosophical Transactions of the Royal Society B: Biological Sciences 2005, 360(1455), 497-505.
- Murray HD, Gourse RL. Unique roles of the rrn P2 rRNA promoters in Escherichia coli. Molecular Microbiology 2004, 52(5), 1375-1387.
- Stokes NR, Murray HD, Subramaniam C, Gourse RL, Louis P, Bartlett W, Miller S, Booth IR. A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS. Proceedings of the National Academy of Sciences of the United states of America 2003, 100(26), 15959-15964.
- Murray HD, Schneider DA, Gourse RL. Control of rRNA Expression by Small Molecules Is Dynamic and Nonredundant. Molecular Cell 2003, 12(1), 125-134.
- Schneider DA, Murray H, Gourse RL. Measuring control of transcription initiation by changing concentrations of nucleotides and their derivatives. Methods in Enzymology 2003, 370, 606-617.
- Murray H, Appleman JA, Gourse RL. Regulation of the Escherichia coli rrnB P2 promoter. Journal of Bacteriology 2003, 185(1), 28-34.