Professor Waldemar Vollmer
Prof of Bacterial Biochemistry

  • Email: waldemar.vollmer@ncl.ac.uk
  • Telephone: +44 (0) 191 208 3216
  • Fax: +44 (0) 191 208 3205
  • Address: The Centre for Bacterial Cell Biology
    Baddiley Clark Building
    Medical School
    Newcastle University
    Richardson Road
    Newcastle upon Tyne
    NE2 4AX
    United Kingdom

Qualifications

Dr. rer. nat. Biochemistry, University of Tübingen, Germany
Dipl. Chemistry, University of Basel, Switzerland
Dipl.-Ing. (FH) Chemistry, Technical College (Fachhochschule) Reutlingen, Germany

Previous Positions

2003 to 2006 University of Tübingen, Germany
2001 to 2002 Max Planck Institute for Developmental Biology, Tübingen, Germany
1998 to 2000 Rockefeller University, New York, USA

Memberships

American Society for Microbiology
Society for General Microbiology
Vereinigung für Allgemeine und Angewandte Mikrobiologie 

Appointments and Awards

2014 Elected Fellow of the American Academy of Microbiology

2014- Editorial Board Journal of Bacteriology

2013 Wellcome Trust Senior Investigator Award

2012 Distinguished Scientist Visiting Scholarship Ben-Gurion University of the Negev, Israel

2012- Editorial Advisory Board Molecular Microbiology

2012- Editorial Advisory Board Antonie van Leeuwenhoek

2003- Editorial Advisory Board Microbial Drug Resistance

2000-2001 Charles H. Revson Fellowship 

 

Research Interests

  • Bacterial cell wall structure and architecture
  • Mechanism of peptidoglycan growth and cell division
  • Regulation of cell wall synthesis and bacterial morphogenesis

Co-workers

Christine Aldridge

Dr. Jacob Varghese Biboy

Lisa Burdis

Dr. Alexander Egan

Dr. Victor Hernandez-Rocamora 

Adam Lodge

Dr. Manuel Pazos

Dr. Katharina Peters

Jad Sassine

Dr. Daniela Vollmer

Sophie Windley 

Current Work

Bacteria have a highly complex cell envelope. The essential peptidoglycan sacculus is a mesh-like cell envelope layer required to protect the cell from rupture due to its turgor and to maintain cell shape. The biosynthetic pathway of peptidoglycan synthesis is the target of our most successful antimicrobials, the beta-lactams and glycopeptides. During growth and cell division the sacculus surface is enlarged by the combined actions of peptidoglycan synthases (penicillin-binding proteins, PBPs) and hydrolases (lytic transglycosylases, amidases and endopeptidases). According to a current model, peptidoglycan enzymes and cell morphogenesis proteins form dynamic multi-enzyme complexes which are regulated by cytoskeletal elements. Escherichia coli and presumably other Gram-negative  bacteria also regulate peptidoglycan growth by outer membrane-anchored lipoproteins that specifically stimulate their cognate, inner membrane-anchored synthase. 

Our research aims to decipher the molecular mechanisms of peptidoglycan growth in the model bacterium Escherichia coli and other Gram-negative and Gram-positive bacteria. We study the activities and interactions of peptidoglycan enzymes and aim to identify novel members of the pathway. We also investigate the spatiotemporal regulation of peptidoglycan growth and the mechanisms of cell shape generation in various species. Finally, we determine the peptidoglycan fine structure (muropeptide composition) and cell wall architecture in Gram-positive and Gram-negative bacteria.

Funding

Biotechnology and Biological Sciences Research Council, European Commission, Medical Research Council, The Royal Society, Wellcome Trust

Publications

102. Tomlinson G, Chimalapati S, Pollard T, Lapp T, Cohen J, Camberlein E, Stafford S, Periselneris J, Aldridge C, Vollmer W, Picard C, Casanova JL, Noursadeghi M, Brown J. 2014. TLR-mediated inflammatory responses to Streptococcus pneumoniae are highly dependent on surface expression of bacterial lipoproteins. Journal of Immunology, in press, doi:10.4049/jimmunol.1401413. 

101. Klöckner A, Otten C, Derouaux A, Vollmer W, Bühl H, De Benedetti S, Münch D, Josten M, Mölleken K, Sahl H-G, Henrichfreise B. 2014. AmiA is a target enzyme of penicillin with dual activity in the intracellular pathogen Chlamydia pneumoniae. Nature Communications 5:4201.

100. Vollmer W. 2014. Peptidoglycan. In: Molecular Medical Microbiology (2nd Edition). Tang YW, Liu D, Poxton IR, Schwartzman JD, Sussman M (Eds.), Elsevier, Waltham, USA. in press.

99. Hoyland C, Aldridge C, Cleverley R, Duchene M, Minasov G, Onopriyenko O, Sidiq K, Stogios P, Anderson W, Daniel R, Savchenko A, Vollmer W, Lewis RJ. 2014. Structure of the LdcB LD-carboxypeptidase reveals the molecular basis of peptidoglycan recognition. Structure 22, 949-960. [Commentary: Cadby IT, Lovreing AL. 2014. Structure 22, 932-934]

98. Jean NL, Bougault CM, Lodge A, Derouaux A, Callens G, Egan AJF, Lewis RJ, Vollmer W*, Simorre J-P*. 2014. The elongated molecular structure of the outer-membrane activator of peptidoglycan synthesis LpoA: implications for PBP1A-stimulation. Structure 22, 1047-1054. [* corresponding authors]

97. Egan AJF#, Jean NL#, Koumoutsi A#, Bougault CM, Biboy J, Sassine J, Solovyova AS, Breukink E, Typas A*, Vollmer W*, Simorre JP*. 2014. Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Proceedings of the National Academy of Sciences USA 111, 8197–8202. [# Contributed equally; * corresponding authors]

96. Jean NL, Bougault CM, Egan AJF, Vollmer W, Simorre JP. 2014. Solution NMR assignment of LpoB, an outer-membrane anchored lipoprotein Penicillin-binding protein activator from Escherichia coli. Biomolecular NMR Assignments, DOI 10.1007/s12104-014-9557-z.

95. Jean NL, Bougault CM, Derouaux A, Callens G, Vollmer W, Simorre J-P. 2014. Backbone and side-chain 1H, 13C, and 15N NMR assignments of the N-terminal domain of Escherichia coli LpoA. Biomolecular NMR Assignments, DOI 10.1007/s12104-014-9546-2.

94. Turner RD, Vollmer W, Foster SJ. 2014. Different walls for rods and balls: The diversity of peptidoglycan. Molecular Microbiology 91, 862-874.

93. Frirdich E, Vermeulen J, Biboy J, Soares F, Taveirne ME, Johnson JG, DiRita VJ, Girardin SE, Vollmer W, Gaynor EC. 2014. Peptidoglycan LD-carboxypeptidase Pgp2 influences Campylobacter jejuni helical cell shape and pathogenic properties, and provides the substrate for the DL-carboxypeptidase Pgp1. Journal of Biological Chemistry 289, 8007-8018.

92. Pilhofer M, Aistleitner K, Biboy J, Gray J, Kuru E, Hall E, Brun Y, VanNieuwenhze M, Vollmer W, Horn M, Jensen G. 2013. Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ. Nature Communications 4:2856. [Research Highlight: Kåhrström CT. 2014. Nature Reviews Microbiology 12, 76]

91. Bernhardt TG, Vollmer W. 2013. Mechanisms of bacterial morphogenesis and their subversion by phages. Current Opinion in Microbiology 16, 728-730. [Editorial Commentary on the themed issue "Growth and development: prokaryotes"]

90. Sycuro LK, Rule, CS, Petersen TW, Wyckoff TJ, Sessler T, Nagarkar DB, Khalid F, Pincus Z, Biboy J, Vollmer W, Salama NR. 2013. Flow cytometry-based enrichment for cell shape mutants identifies multiple genes that influence Helicobacter pylori morphology. Molecular Microbiology 90, 869–883.

89. Srikannathasan V, English G, Bui NK, Trunk K, O’Rourke PEF, Rao VA, Vollmer W, Coulthurst SJ, Hunter WN. 2013. Structural basis for Type VI secreted peptidoglycan DL-endopeptidase function, specificity and neutralization in Serratia marcescens. Acta Crystallographica Section D: Biological Crystallography 69, 2468-2482.

88. Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD. 2013. Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector–immunity pair. Journal of Biological Chemistry 288, 26616-26624.

87. Massidda O, Nováková L, Vollmer W. 2013. From models to pathogens: how much have we learned about Streptococcus pneumoniae cell division? Environmental Microbiology 15, 3133-3157.

86. Derouaux A, Terrak M, den Blaauwen T, Vollmer W. 2013. Bacterial cell wall growth, shape and division. In: Bacterial Membranes: Structural and Molecular Biology. Remaut H, Fronzes R (Eds.), Horizon Scientific Press and Caister Academic Press, Norwich, UK. pp. 3-54.

85. Shin JY, Vollmer W, Lagos R, Monasterio O. 2013. Glutamate 83 and arginine 85 of helix H3 bend are key residues for FtsZ polymerization, GTPase activity and cellular viability of Escherichia coli: lateral mutations affect FtsZ polymerization and E. coli viability. BMC Microbiology 13:26.

84. Takacs CN, Hocking J, Cabeen MT, Bui NK, Poggio S, Vollmer W, Jacobs-Wagner C. 2013. Growth medium-dependent glycine incorporation into the peptidoglycan of Caulobacter crescentus. PloS One 8(2): e57579. 

83. van der Ploeg R, Verheul J, Vischer NOE, Alexeeva S, Hoogendoorn E, Postma M, Banzhaf M, Vollmer W, den Blaauwen T. 2013. Colocalization and interaction between elongasome and divisome during a preparative cell division phase in Escherichia coli. Molecular Microbiology 87, 1074-1087. 

82. Biboy J, Bui NK, Vollmer W. 2013. In vitro peptidoglycan synthesis assay with lipid II substrate. In: Bacterial Cell Surfaces: Methods and Protocols, Methods in Molecular Biology, vol. 966, Delcour AH (Ed.), pp. 273-288.

81. Egan A, Vollmer W. 2013. The physiology of bacterial cell division. In: Annals of the New York Academy of Sciences 1277, issue "Antimicrobial Therapeutics Reviews". Bush K (Ed.), The New York Academy of Sciences, pp. 8-28.

80. Vollmer W. 2012. Murein peptide amidase MpaA. In: Handbook of Proteolytic Enzymes 3rd Edition, Rawlings N, Salvesen G (Eds.), Elsevier, Oxford, UK. pp. 1387-1389. 

79. Vollmer W. 2012. Bacterial growth does require peptidoglycan hydrolases. Molecular Microbiology 86, 1031-1035. [Commentary on: Singh et al. 2012. Molecular Microbiology 86, 1036-1051]

78. Chou S, Bui NK, Russell AB, Lexa K, LeRoux M, Vollmer W*, Mougous JD*. 2012. Structure of a peptidoglycan amidase effector targeted to Gram-negative bacteria by the type VI secretion system. Cell Reports 1, 656-664. [* corresponding authors]

77. Banzhaf M, van den Berg van Saparoea B, Terrak M, Fraipont C, Egan A, Philippe J, Zapun A, Breukink E, Nguyen-Distèche M, den Blaauwen T, Vollmer W. 2012. Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Molecular Microbiology 85, 179-194.

76. Hocking J, Priyadarchini R, Takacs CN, Costa T, Dye NA, Shapiro L, Vollmer W, Jacobs-Wagner C. 2012. Osmolality-dependent relocation of penicillin-binding protein PBP2 to the division machinery in Caulobacter crescentus. Journal of Bacteriology 194, 3116-3127.

75. Russell AB, Singh P, Brittnacher M, Bui NK, Hood RD, Carl MA, Agnello DM, Schwarz S, Goodlett DR, Vollmer W, Mougous JD. 2012. A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host & Microbe 11, 538-549. 

74. Helassa N, Breukink E, Vollmer W, Vernet T, Zapun A. 2012. The membrane anchor of penicillin-binding protein PBP2a from Streptococcus pneumoniae influences peptidoglycan chain length. FEBS Journal 279, 2071-2081.

73. Denapaite D, Brückner R, Hakenbeck R, Vollmer W. 2012. Biosynthesis of teichoic acids in Streptococcus pneumoniae and closely related species: lessons from genomes. Microbial Drug Resistance 18, 344-358.

72. Eberhardt A, Hoyland CN, Vollmer D, Bisle S, Cleverley RM, Johnsborg O, Håvarstein LS, Lewis RJ, Vollmer W. 2012. Attachment of capsular polysaccharide to the cell wall in Streptococcus pneumoniae. Microbial Drug Resistance 18, 240-255.

71. Osborne SE, Tuinema BR, Mok MCY, Lau PS, Bui NK,Tomljenovic-Berube AM, Vollmer W, Zhang K, Junop M, Coombes BK. 2012. Characterization of DalS, an ATP-binding Cassette Transporter for D-alanine, and its role in pathogenesis in Salmonella enterica. Journal of Biological Chemistry 287, 15242-15250.

70. Frirdich E, Biboy J, Adams C, Lee J, Ellermeier J, Davis Gielda L, DiRita VJ, Girardin SE, Vollmer W, Gaynor EC. 2012. Peptidoglycan-modifying enzyme Pgp1 is required for helical cell shape and pathogenicity traits in Campylobacter jejuni. PloS Pathogens 8(3): e1002602.

69. Sycuro LK, Wyckoff TJ, Biboy J, Born P, Pincus Z, Vollmer W, Salama NR. 2012. Multiple peptidoglycan modification networks modulate Helicobacter pylori’s cell shape, motility, and colonization potential. PloS Pathogens 8(3): e1002603. 

68. Lerner TR, Lovering AL, Bui NK, Uchida K, Aizawa S-I, Vollmer W, Sockett RE. 2012. Specialized peptidoglycan hydrolases sculpt the intra-bacterial niche of predatory Bdellovibrio and increase population fitness. 2012. PloS Pathogens 8(2): e1002524. 

67. Figueiredo TA, Sobral RG, Ludovice AM, de Almeida JMF, Bui NK, Vollmer W, de Lencastre H, Tomasz A. 2012. Identification of genetic determinants and enzymes involved with the amidation of glutamic acid residues in the peptidoglycan of Staphylococcus aureus. PloS Pathogens 8(1): e1002508.

66. Typas A, Banzhaf M, Gross CA, Vollmer W. 2012. From the regulation of peptidoglycan synthesis to bacterial growth and morphology. Nature Reviews Microbiology 10, 123-136.

65. Vollmer W. 2012. Bacterial outer membrane evolution via sporulation? Nature Chemical Biology 8, 14-18. [Commentary on: Tocheva EI et al. 2011. Cell 146, 799-812]

64. Bui NK, Eberhardt A, Vollmer D, Kern T, Bougault C, Tomasz A, Simorre J-P, Vollmer W. 2012. Isolation and analysis of cell wall components from Streptococcus pneumoniae. Analytical Biochemistry 421, 657-666.

63. Kawai Y, Marles-Wright J, Cleverly RM, Emmins R, Ishikawa S, Kuwano M, Heinz N, Bui NK, Hoyland CN, Ogasawara N, Lewis RJ, Vollmer W, Daniel RA, Errington J. 2011. A widespread family of bacterial cell wall assembly proteins. EMBO Journal 30, 4931-4941. [Research Highlight: Molloy S. 2011. Nature Reviews Microbiology 9, 768]

62. Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD. 2011. Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475, 343-347. [Commentary: Cotter P. 2011. Nature 475, 301-303; Research Highlight: Huddleston JE. 2011. Nature Reviews Microbiology 9, 630]

61. Schäberle T, Vollmer W, Frasch H-J, Hüttel S, Kulik A, Röttgen M, von Thaler AK, Wohlleben W, Stegmann E. 2011. Self-resistance and cell wall composition in the glycopeptide producer Amycolatopsis balhimycina. Antimicrobial Agents and Chemotherapy 55, 4283-4289.

60. Bisicchia P, Bui NK, Aldridge C, Vollmer W, Devine KM. 2011. Acquisition of VanB-type vancomycin resistance by Bacillus subtilis: the impact on gene expression, cell wall composition and morphology. Molecular Microbiology 81, 157-178.     

59. Bui NK, Turk S, Buckenmaier S, Stevenson-Jones F, Zeuch B, Gobec S, Vollmer W. 2011. Development of screening assays and discovery of initial inhibitors of pneumococcal peptidoglycan deacetylase PgdA. Biochemical Pharmacology 82, 43-52.

58. Olrichs NK, Aarsman MEG, Verheul J, Arnusch CJ, Martin NI, Hervé M, Vollmer W, de Kruijff B, Breukink E, den Blaauwen T. 2011. A novel in vivo cell wall labeling approach sheds new light on peptidoglycan synthesis in Escherichia coli. ChemBioChem 12, 1124-1133.

57. Typas A#*, Banzhaf M#, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink B, den Blaauwen T, Gross CA, Vollmer W*. 2010. Regulation of peptidoglycan synthesis by outer-membrane proteins. Cell 143, 1097-1109. [# Contributed equally; * corresponding authors; commentary: Young KD. 2010. Cell 143, 1042-1044.]

56. Kern T, Giffard M, Hediger S, Amoroso A, Giustini C, Bui NK, Joris B, Bougault C, Vollmer W, Simorre J-P. 2010. Dynamics characterization of fully hydrated bacterial cell walls by solid-state NMR: evidence for cooperative binding of metal ions. Journal of the American Chemical Society 132, 10911-10919.

55. Potluri L, Karczmarek A, Verheul J, Piette A, Wilkin J-M, Werth N, Banzhaf M, Vollmer W, Young KD, Nguyen-Distèche M, den Blaauwen T. 2010. Septal and lateral wall localization of PBP5, the major D,D-carboxypeptidase of Escherichia coli, requires substrate recognition and membrane attachment. Molecular Microbiology 77, 300-323.

54. Poggio S, Takacs CN, Vollmer W, Jacobs-Wagner C. 2010. A protein critical for cell constriction in the Gram-negative bacterium Caulobacter crescentus localizes at the division site through its peptidoglycan-binding LysM domains. Molecular Microbiology 77, 74-89. [Commentary: Collier J. 2010. Molecular Microbiology 77, 11-14.]

53. Cabeen MT, Murolo MA, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C. 2010. Mutations in the lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus. Journal of Bacteriology 192, 3368-3378.

52. Sycuro LK, Pincus Z, Gutierrez KD, Biboy J, Stern CA, Vollmer W, Salama NR. 2010. Peptidoglycan crosslinking relaxation promotes Helicobacter pylori’s helical shape and stomach colonization. Cell 141, 822-833. [Commentary: Dworkin J. 2010. Molecular Microbiology 78, 792-795.]

51. Pérez-Dorado I, González A, Morales M, Sanles R, Hidalgo A, Striker W, Vollmer W, Mobashery S, García JL, Martínez-Ripoll M, García P, Hermoso JA. 2010. Insights into pneumococcal fratricide from crystal structure of the modular killing factor LytC. Nature Structural & Molecular Biology 17, 576-582.

50. Takacs CN, Poggio S, Charbon G, Pucheault M, Crews C, Vollmer W, Jacobs-Wagner C. 2010. MreB drives de novo rod morphogenesis in Caulobacter crescentus via remodeling of the cell wall. Journal of Bacteriology 192, 1671-1684.

49. Vollmer W, Seligman SJ. 2010. Architecture of peptidoglycan: more data and more models. Trends in Microbiology 18, 59-66.

48. Eberhardt A, Wu LJ, Errington J, Vollmer W*, Veening J-W*. 2009. Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Molecular Microbiology 74, 395-408. [* corresponding authors]

47. Gehre F, Spisek R, Kharat AS, Matthews P, Kukreja A, Anthony RM, Dhodapkar MV, Vollmer W, Tomasz A. 2009. The role of teichoic acid choline moieties in the virulence of Streptococcus pneumoniae. Infection and Immunity 77, 2824-2831.

46. Cabeen MT, Charbon G, Vollmer W, Born P, Ausmees N, Weibel DB, Jacobs-Wagner C. 2009. Bacterial cell curvature through mechanical control of cell growth. EMBO Journal 28, 1208-1219. [Commentary: de Boer PA. 2009. EMBO Journal 28,1193-1194.]

45. Vollmer W. 2009. Murein: Das dynamische Exoskelett der Bakterien. BIOspektrum 15, 248-251.

44. Guo YL, Mayer H, Vollmer W, Dittrich D, Sander P, Schultz A, Schultz JE. 2009. Polyphosphates from Mycobacterium bovis - potent inhibitors of class III adenylyl cyclases. FEBS Journal 276, 1094-1103.

43. Baur S, Marles-Wright J, Buckenmaier S, Lewis RJ, Vollmer W. 2009. Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae. Journal of Bacteriology 191, 1200-1210.

42. Bui NK, Gray J, Schwarz H, Schumann P, Blanot D, Vollmer W. 2009. The peptidoglycan sacculus of Myxococcus xanthus has unusual structural features and is degraded during glycerol-induced myxospore development. Journal of Bacteriology 191, 494-505.

41. Vollmer W, Born P. 2009. Bacterial cell envelope peptidoglycan. In: Microbial Glycobiology. Moran A, Holst O, Brennan P, von Itzstein M (Eds.) Elsevier, San Diego, USA. pp. 15-28.

40. Fittipaldi N, Sekizaki T, Takamatsu D, de la Cruz Domínguez-Punaro M, Harel J, Bui NK, Vollmer W, Gottschalk M. 2008. Significant contribution of the pgdA gene to the virulence of Streptococcus suis. Molecular Microbiology 70, 1120-1135.

39. Kharat A, Denapaite D, Gehre F, Brückner R, Vollmer W, Hakenbeck R, Tomasz A. 2008. Different pathways of choline metabolism in two choline-independent strains of Streptococcus pneumoniae and their impact on virulence. Journal of Bacteriology 190, 5907-5914.

38. Kern T, Hediger S, Mϋller P, Giustini C, Joris B, Bougault C, Vollmer W, Simorre J-P. 2008. Towards the characterization of peptidoglycan structure and protein – peptidoglycan interactions by solid-state NMR spectroscopy. Journal of the American Chemical Society 130, 5618-5619.

37. Vollmer W. 2008. Targeting the bacterial Z-ring. Chemistry & Biology 15, 93-94.

36. Vollmer W, Joris B, Charlier P, Foster S. 2008. Peptidoglycan (murein) hydrolases. FEMS Microbiology Reviews 32, 259-286.

35. Vollmer W. 2008. Structural variation in the glycan strands of bacterial peptidoglycan. FEMS Microbiology Reviews 32, 287-306.

34. Vollmer W, Blanot D, de Pedro MA. 2008. Peptidoglycan structure and architecture. FEMS Microbiology Reviews 32, 149-167.

33. Vollmer W, Bertsche U. 2008. Murein (peptidoglycan) structure, architecture and biosynthesis in Escherichia coli. Biochimica et Biophysica Acta 1778, 1714-1734.

32. Müller P, Ewers C, Bertsche U, Anstett M, Kallis T, Breukink E, Fraipont C, Terrak M, Nguyen-Distèche M, Vollmer W. 2007. The essential cell division protein FtsN interacts with the murein (peptidoglycan) synthase PBP1B in Escherichia coli. Journal of Biological Chemistry 282, 36394-36402.

31. Damjanovic M, Kharat A, Eberhardt A, Tomasz A, Vollmer W. 2007. The essential tacF gene is responsible for the choline-dependent growth phenotype of Streptococcus pneumoniae. Journal of Bacteriology 189, 7105-7111.

30. Aaron M, Charbon G, Lam H, Schwarz H, Vollmer W*, Jacobs-Wagner C*. 2007. The tubulin homologue FtsZ contributes to cell elongation by guiding cell wall precursor synthesis in Caulobacter crescentus. Molecular Microbiology 64, 938-952. [* corresponding authors; commentary: Scheffers DJ. 2007. Molecular Microbiology 64, 877-880.]

29. Vollmer W. 2007. Preparation and analysis of pneumococcal murein (peptidoglycan). In: Molecular Biology of Streptococci. Hakenbeck R, Chhatwal S (Eds.), Horizon Scientific Press, Norfolk, UK, 531-535.

28. Vollmer W. 2007. Structure and biosynthesis of the pneumococcal cell wall. In: Molecular Biology of Streptococci. Hakenbeck R, Chhatwal S (Eds.), Horizon Scientific Press, Norfolk, UK, pp. 83-117.

27. Vollmer W. 2006. Structure and biosynthesis of the murein (peptidoglycan) sacculus. In: The periplasm. Ehrmann M (Ed.), ASM Press, Washington, USA, pp. 198-213.

26. Vollmer W. 2006. The prokaryotic cytoskeleton: A putative target for inhibitors and antibiotics? Applied Microbiology and Biotechnology 73, 37-47.

25. Crisóstomo MI, Vollmer W, Kharat A, Inhülsen S, Gehre F, Buckenmaier S, Tomasz A. 2006. Attenuation of penicillin resistance in a peptidoglycan O-acetyl transferase mutant of Streptococcus pneumoniae. Molecular Microbiology 61, 1497-1509.

24. Born P, Breukink E, Vollmer W. 2006. In vitro synthesis of cross-linked murein (peptidoglycan) and its attachment to sacculi by PBP1A from Escherichia coli. Journal of Biological Chemistry 281, 26985-26993.

23. Kovacs M,  Halfmann A, Fedtke I, Heintz M, Peschel A, Vollmer W, Hakenbeck R,  Brückner R. 2006. A functional dlt operon, encoding proteins required for incorporation of D-alanine in teichoic acids in gram-positive bacteria, confers resistance to cationic antimicrobial peptides in Streptococcus pneumoniae. Journal of Bacteriology 188, 5797-5805. 

22. Bertsche U, Kast T, Fraipont C, Wolf B, Aarsman MEG, Kannenberg K, von Rechenberg M, Nguyen-Distèche M, den Blaauwen T, Höltje J-V, Vollmer W. 2006. Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli. Molecular Microbiology 61, 675-690.

21. Vollmer W. 2006. Cytoskeletal elements in prokaryotes. In: Microbiology Monographs, Volume 2: Complex Intracellular Structures in Prokaryotes. Shively J (Ed.), Springer, Heidelberg, pp. 305-311.

20. Bertsche U, Breukink E, Kast T, Vollmer W. 2005. In vitro murein (peptidoglycan) synthesis by dimers of the bifunctional transglycosylase-transpeptidase PBP1B from Escherichia coli. Journal of Biological Chemistry 280, 38096-38101.

19. van Straaten KE, Dijkstra BW, Vollmer W, Thunnissen A-MWH. 2005. Crystal structure of MltA from Escherichia coli reveals a unique lytic transglycosylase fold. Journal of Molecular Biology 352, 1068-1080.

18. Korsak D, Vollmer W, Markiewicz Z. 2005. Listeria monocytogenes EGD lacking penicillin-binding protein 5 (PBP5) produces a  thicker cell wall. FEMS Microbiology Letters 251, 281-288.

17. Korsak D, Liebscher S, Vollmer W. 2005. Susceptibility to antibiotics and ß-lactamase induction in murein hydrolase mutants of Escherichia coli. Antimicrobial Agents and Chemotherapy 49, 1404-1409.

16. Bera A, Herbert S, Jakob A, Vollmer W, Götz F. 2005. Why are pathogenic staphylococci so lysozyme resistant? The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus. Molecular Microbiology 55, 778-787.

15. Ursinus A, van den Ent F, Löwe J, de Pedro MA, Höltje J-V, Vollmer W. 2004. Murein (peptidoglycan) binding property of the essential cell division protein FtsN from Escherichia coli. Journal of Bacteriology 186, 6728-6737.

14. Vollmer W, Höltje J-V. 2004. The architecture of murein (peptidoglycan) in Gram-negative bacteria: vertical scaffold or horizontal layer(s)? Journal of Bacteriology 186, 5978-5987.

13. Meisel U, Höltje J-V, Vollmer W. 2003. Overproduction of inactive variants of the murein synthase PBP1B causes lysis in Escherichia coli. Journal of Bacteriology 185, 5342-5348.

12. Vollmer W, Tomasz A. 2002. Peptidoglycan N-acetylglucosamine deacetylase (PgdA) - a putative virulence factor in Streptococcus pneumoniae. Infection and Immunity 70, 7176-7178.

11. Heidrich C, Vollmer W. 2002. Murein (Peptidoglycan). In: Biopolymers. Volume 5: Polysacccharides I. Steinbuechel A (Ed.), Wiley-VHC, Weinheim, pp. 431-463.

10. Vollmer W, Höltje J-V. 2001. Morphogenesis of Escherichia coli. Current Opinion in Microbiology 4, 625-633.

9. von Rechenberg M, Vollmer W, Höltje J-V. 2001. A multienzyme complex involved in murein synthesis of Escherichia coli. In: Microbial Fundamentals of Biotechnology, Braun V, Götz F (Eds.), Wiley-VCH, Weinheim, pp. 249-262.

8. Vollmer W, Tomasz A. 2001. Identification of the teichoic acid phosphorylcholine esterase in Streptococcus pneumoniae. Molecular Microbiology 39, 1610-1622.

7. Vollmer W, Tomasz A. 2000. The pgdA gene encodes for a peptidoglycan N-acetylglucosamine deacetylase in Streptococcus pneumoniae. Journal of Biological Chemistry 275, 20496-20501.

6. Vollmer W, Höltje J-V. 2000. A simple screen for murein transglycosylase inhibitors. Antimicrobial Agents and Chemotherapy 44, 1181-1185.

5. Vollmer W, von Rechenberg M, Höltje J-V. 1999. Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli. Journal of Biological Chemistry 274, 6726-6734.

4. Höltje J-V, Schiffer G, Vollmer W, von Rechenberg M. 1998. Growth of the bacterial sacculus requires both polymerization and depolymerization of murein. In: Biochemical Principles and Mechanisms of Biosynthesis and Biodegradation of Polymers, Proceedings of an International Symposium held at the Westfälische Wilhelms-Universität, Münster, Germany, June 3-6, 1998, Steinbüchel A (Ed.), Wiley-VCH, Weinheim, pp. 68-76.

3. Lommatzsch J, Templin MF, Kraft AR, Vollmer W, Höltje J-V. 1997. Outer membrane localization of murein hydrolases: MltA, a third lipoprotein lytic transglycosylase in Escherichia coli. Journal of Bacteriology 179, 5465-5470.

2. Vollmer W, Pilsl H, Hantke K, Höltje J-V, Braun V. 1997. Pesticin displays muramidase activity. Journal of Bacteriology 179, 1580-1583.

1. Romeis T, Vollmer W, Höltje J-V. 1993. Characterization of three different lytic transglycosylases in Escherichia coli. FEMS Microbiology Letters 111, 141-146. 

Undergraduate Teaching

Teaching in MIC2026 and MIC3044

CMB3000 project

Postgraduate Teaching

Teaching in MRes modules MMB8016 (Molecular Microbiology, module leader), MMB8018 (Protein Structure and Function)

Masters and Ph.D. supervisor