Project:

Cellular senescence - mitochondrial dysfunction


Project Leader(s): Thomas von Zglinicki
Staff: Glyn Nelson, Joao Passos, Satomi Miwa, Darren Daniels, Gabriele Saretzki
Sponsors: BBSRC and EPSRC
Partners: Mitochondrial Research Group - http://www.ncl.ac.uk/biomedicine/research/groups/mitochondrial.htm

In cellular senescence, various signals trigger mitochondrial dysfunction, i.e. increased ROS production together with decreased membrane potential. Joao Passos, Glyn Nelson and Satomi Miwa characterize this dysfunction by flow cytometry, life cell imaging and oxygraphy, and Darren Daniels measures mtDNA damage. Gabriele Saretzki has shown a protective function for telomerase in mitochondria, and is now interested in its role during stem cell ageing and differentiation.

Fluorescence microscopy image showing mitochondria in senescent human fibroblasts.  All mitochondria are stained green, and those which are active are also stained red, demonstrating a large number of non-functioning (green) mitochondria in many of the senescent cells.  Nuclei are stained blue.

For more information contact:

Glyn Nelson
email: glyn.nelson@ncl.ac.uk

Staff

Dr Glyn Nelson
Senior Research Associate

Dr Gabriele Saretzki
Lecturer

Professor Thomas von Zglinicki
Professor of Cellular Gerontology