Transmission Electron Microscopy (TEM)
Transmission electron microscopy allows the visualisation of cell ultrastructure.
The lab houses a Philips CM100 TEM with Compustage and high resolution digital image capture. This allows very accurate measuring, image collection, montage and random sampling. Experienced staff are here to provide support in all areas of TEM.
Specialised techniques:
- Standard epoxy resin embedding
- Negative staining
- Shadowing
- Immunogold both resin and cryo
- Freeze substitution
- Pre-embedding immunogold
- Pre-embedding enzyme histochemistry
- Stereology
Typical applications for TEM:
- Plant and animal tissue and cell culture
- Isolated proteins
- Monolayers
- Bacteria and viruses
- Detergent-extracted cytoskeleton and organelles
- Particulate size and distribution in non-biological samples
Check out our training packages which are available for researchers and which will reduce costs in the long term.
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