Flow Cytometry Core Facility Equipment

The facility contains instrumentation as below;

Customised 4 Laser FACSAria II cell sorter

This sorter has 4 lasers , they are Blue 488nm, Red 635nm,Violet 405nm and UV 355nm

The preferred fluorochromes for each detector are below, however many other combinations are possible , for advice please contact us .

(Laser-Band pass/Tolerance)

488-780/60 488-710/50 488-660/20 488-610/20 488-575/26 488-520/20
PE-Cy7 PerCP-eFluor 710   PE-CF594 Pe FITC
635-780/60 635-730/45 635-670/14
APC-H7 Alexa 700 APC
405-655/8 405-525/50 405-450/50
BV650 BV510 BV421
355-675lp 355-450/50
  Alexa 350

Customised 5 laser LSR II analysis instrument

This instrument has 5 lasers, they are Blue 488nm, Red 635nm,Violet405nm, Green 532nm and UV 355nm

(Laser-Band pass/Tolerance)

488-780/60 488-710/50 488-660/20 488-610/20 488-575/26 488-520/20
PE-Cy7 PerCP-eFluor 710   PE-CF594 Pe FITC
635-780/60 635-730/45 635-670/14
APC-H7 Alexa 700 APC
405-655/8 405-525/50 405-450/50
BV650 BV510 BV421
355-675lp 355-450/50
  Alexa 350
532-710/50 532-585/42
PeCy5.5 Alexa 532

4 FACSCanto II flow cytometers.

Two are 3 laser (Blue, Red, Violet) producing 8 colour capability

Two are 2 laser (Blue and Red) producing 6 colour capability

(Laser-Band pass/Tolerance)

488-780/60 488-670lp 488-585/42 488-530/30
PeCy7 PerCP-eFluor 710 PE FITC
635-780/60 635-660/20
APC-H7 APC
405-510/50 450/50
BV510 BV421

 

Tali Image Based Cytometer

This instrument has 3 Chanells 2 of which are capable of fluorescence detection

1-Bright filed, 2Green Fluorescence 3,Red Fluorescence

Fluorescence parameter Green Fluorescence Red Fluorescence
Ex-Em/tolerance 458- 525/50 530-585/lp
  Viability kit Dead Cell Green Viability kit Dead Cell Red

A list of dyes that can be used with this instrument can be found here

The Bright field is used for cell counting

FACSFusion Sorter

To be updated

Fortessa X20

To be updated

 

Accessory instrumentation includes;

  • Lyse wash assistants
  • High throughput sampler module for use with the LSR II
  • Vi Cell cell counter.

A comprehensive suite of flow Cytometry analysis software is also available on each of the two sites (Centre for life and Wm Leech Building ) to enable you to analyse acquired data quickly and efficiently, remote from the instruments.

The facility has a particular emphasis on flow cytometry education and we deliver both basic and advanced flow cytometry training. In addition.

All core facility staff strive to offer help and assistance to all users whether it's to assist in experimental set up, fluorochrome choice or any other issues that impact on the use of the facility.

We are happy to discuss any flow cytometry based work, or educational requirements from internal or external customers.

Software for the analysis of flow cytometry data can be found in both Laboratories situated on a stand alone analysis computer, you can not book these computers so please be aware that you may have to wait to use them

Software available includes