Newcastle University

Introduction

I am a molecular biologist/geneticist with continuous research experience in the fields of ageing and cancer for more than 15 years. Most of my work concentrates on telomerase and oxidative stress. I am interested in the role of oxidative stress and mitochondria in the ageing process and cellular senescence. We have shown a protective role for telomerase/TERT for mitochondrial function and decrease of oxidative stress. We also use in vivo models of TERT knockout mice and dietary restriction to analyse this protective function of telomerase. That could have far reaching consequences for the better understanding of ageing and age-related diseases.

Background

I am a cellular biologist with a broad range of experience, interests and technical skills. My main scientific career has been devoted to investigating the involvement of oxidative stress, telomeres and telomerase in processes connected to cellular ageing, cancer and stem cells. For my doctoral research, I worked in molecular virology, expressing surface domains of the hepatitis B virus in different microorganisms for application as vaccines against Hepatitis B. During that time I prepared and taught a half year laboratory course in general and molecular genetics. For my first post-doc years I worked in diagnostic pathology, introducing new methods like in-situ hybridization, immunohistochemistry and PCR into pathological diagnostics. In the context of cancer pathology, I analysed translocations ,microsatellite instabilities, losses of heterozygosity [3, 9] and gene amplifications .
For the last 15 years my main research interests have been devoted to the role of oxidative stress, telomeres and telomerase in ageing, cancer and stem cells as well. In close collaboration with other scientists I have investigated the role of senescence, oxidative stress and telomeres in the basic biology of ageing as well as related clinical questions.

Roles and Responsibilities

•Editorial member of “Biogerontology” since 2002, Associated Editor for BMC Biology since 2009 and Oxidative Medicine since 2011
•Member of NESCI (North East Stem Cell Institute), Associate member of the Institute for Genetics medicine and the Northern Institute for Cancer Research (NICR) •Reviewer for different funding bodies (BBSRC, Wellcome, MRC etc) and journals:Mechanism of Aging and Development, Ageing Cell,Experimental Gerontology,Biogerontology,Experimental Cell Research,International Journal of Cancer,Molecular Cancer,Reviews in Medical Chemistry, Cancer Letters, PLos one, BMC, NAR etc

Qualifications

1977-1982 Diploma (MSc) in Biology/Genetics, Department of Genetics and Selection, University of St. Petersburg, Russia: “Characterisation of recessive suppressor mutants depending on cyclohexamide in S. cerevisiae”(magna cum laude)
1982-1986 Annual Courses for Young Researchers in Molecular Biology at the Max-Delbrűck Center, Berlin
1986 Postgraduate summer-Course in Genetics, Department of Genetics and Selection, University of St. Petersburg, Russia
1986-1990 PhD in Molecular Biology, Department of Genetics, Humboldt University Berlin, Germany: "Expression of surface antigens of Hepatitis B virus in bacteria and yeast” (magna cum laude), Supervisor Prof. T. Bőrner
1989-1990 1 year Course in Pathology, Institute of Pathology, Charite’, Humboldt-University Berlin
1994-1995 Postdoctoral Research Fellowship, Institute of Pathology of the Technical University and Research Institute (GSF), Munich, Germany
7-9/1996 Research Fellow/ Visiting Scientist, Geron Corp. Menlo Park, CA, USA

Previous Positions

1982-86 Junior Research Associate, Department of Genetics, Humboldt-University Berlin, Germany
1986-1989PhD student, Department of Genetics, Humboldt- University Berlin, Germany
1989-99 Research Associate, Institute of Pathology, Charite’, Humboldt-University Berlin
1999-2000Research Associate, Department of Gynecology, University Hospital, Freiburg, Germany
2001-2002Senior Research Associate, Gerontology, Institute for Ageing and Health, University of Newcastle, U.K.
2002-presentLecturer, Crucible Lab, Institute for Ageing and Health, Newcastle University, U.K.

Memberships

German Association of Geriatrics and Gerontology

Honours and Awards

•1994-1995 Research Fellowship Jung-Foundation for Science, Hamburg, Germany
•1996 (3 month) Research Fellowship Geron Corp. Menlo Park, CA

Languages

German, English, Russian, 

Informal Interests

I love cats and animals, I support WWF, Animal Asia and the RSPCA, I am interested in environmental issues, like sports (windsurfing, climbing, dancing)and travelling.

Research Interests

My research interests are devoted to the role of telomerase in ageing and cancer and under oxidative stress.  I focus particularly on non-telomeric and non-canonical roles of telomerase. We and others have found that telomerase shuttles from the nucleus to mitochondria under oxidative stress. My group was the first to describe a protective role of telomerase and its protein part TERT onto mitochondria. I am working on the molecular mechanisms for this protective function currently.

I am also interested in the mechanisms of stem cell ageing and senescence and investigate the role of telomeres, telomerase and oxidative stress in those. 

Other Expertise

•regular reviews for different funding bodies (UK: BBSRC, Wellcome, MRC, USA, Chech Republic, etc).

•Reviewer for different journals: PLos one, NAR, Mechanism of Aging and Development,
Ageing Cell, Experimental Gerontology, Biogerontology, Experimental Cell Research, International Journal of Cancer, Molecular Cancer, Cancer Letters

I am currently introducing new methods for measuring cognitive properties such as Barnes maze (learning and memory) and Novel object recognition for mice in our institute. 

 
Current Work

I am shifting my focus now to the analysis of possible role of telomerase/TERT in the brain and for brain ageing as well as for the development of neurodegenerative diseases.

I am using human brain material from the NBTR as well as using a TERT knockout mouse model. We are cultivation mouse neurons and neural crest stem cells as in vitro models and are interested in the influence of non-telomeric functions of TERT on mitochondrial properties and oxidative stress parameters.

Future Research

I have various ongoing research collaborations, including work on human embryonic and adult stem cells, as well as iPS cells.

I have a new collaboration with investigators in the field of Alzheimers' research from our institute due to the involvement of oxidative stress and mitochondrial dysfuntion and a possible protective role of telomerase/TERT in the disease.

Research Roles

I run my own lab in the "Crucible" , based at the Bioscience Centre of the International Centre for Life.

Postgraduate Supervision

I have currently 3 PhD students , have regular national and international undergraduate and graduate students and scientists who  work in placements, masters, undergraduate and summer student projects and perform scientific visits to my lab.

Esteem Indicators

speaker and session chair for various national and international meetings on Ageing etc

Funding

Completed:

1997-2000, Cancer Society Berlin: Telomerase combination therapy £200k

•1988-1999 Humboldt University Berlin, Medical Faculty (Charite) Research fund: “Diagnostic potential of telomerase activity for early stages of cervical intraepithelial lesions”, £10k
•2001-2003 Medical Research Council, U.K.: “Telomerase inhibition in prostate cancer” (part of Cooperative Grant on Prostate Cancer) 1/02 - 12/03, £140k
•2003-2004: National Health Trust (Special Trustees): “Mechanism of fast apoptosis induction by targeting telomerase with an anti-hTERT ribozyme in human cancer cells”, £30K

•2003-2008 Co- Investigator for Research into Ageing (now Age UK) Programme “Human cell replicative senescence as stress response: Role of telomeres” , £398K

•2005-2010 named researcher on BBSRC Grant Centre for Integrated Systems Biology of Ageing and Nutrition (CISBAN) for Biogerontology, Institute for Ageing and Health, School of Clinical medical Sciences, University Newcastle, £6.7million
•2005-2006 Co-Investigator for Grant of the National Health Trust (Special Trustees): “The Role of Oxidative Stress in the Phenotypic and Functional Change Occurring in Lung Epithelial Cells undergoing Epithelial to Mesenchymal Transition (EMT)”, £50K

•2005-2008 Co-Investigator for Cancer UK studentship “Identification and validation of candidate genes involved in differentiation of human prostate epithelial stem cells” £70k

2008-2009: co-applicant : X-ray research irradiation system, Ref BB/F010966/1, £141849.91, BBSRC,  

Active: 

2009-2011, co-applicant on grant from "Wellbeing of women", The role of the identified regulators of cell fate (RCF) and metastasis inducing proteins (MIP) in endometrial stem/progenitor cells (SPC) in endometriosis.  £149K

2009-2012, co-applicant with Prof. Majlinda Lako “Investigating early haematopoietic development in ligase IV and XLF patients using induced pluripotent stem cells”. £165.845, Leukaemia Research Fund.

2008-2012, PhD studentship, £78K from Thai government

2009-2012 PhD studentship, 50K, BBSRC

2010-2013, PhD studentship, 50K from Biomedical Research Centre at Newcastle University

Patents

von Zglinicki T, Ludwig A, Saretzki G “Ribozymes targeting the catalytic subunit of human telomerase (hTERT)”, German Patent # 199 03 961.5, International patent PCT/DE 100 03
356

Projects

• Does mitochondrial telomerase protect the brain against ageing and age-related neurodegenerative diseases?
  Project Leader(s): Dr. Gabriele Saretzki
• Is telomerase involved in stem cell ageing?
  Project Leader(s): Dr. Gabriele Saretzki

Undergraduate Teaching

1984-89 Mentor for undergraduates in molecular biology, Institute of Biology, Humboldt-University Berlin
1984-1985 Conception and teaching of a laboratory course “Molecular Genetics” for undergraduate students in Biology
1985-87 Lectures in Molecular Biology, Institute of Biology, Humboldt-University Berlin
since 2003- regular project supervisor for undergraduate stage 3 projects
since 2009-teaching in the MRes course "Ageing" at Newcastle University
since 2010- teaching in the new undergraduate model "Biology of ageing"

since 2011: teaching in masters course "Stem cells and regenerative medicine"  

supervision of 5 summer students 

Postgraduate Teaching

1993-1999 Supervision of MSc, PhD and MD students, Institute of Pathology, Humboldt-University Berlin
1996 - 1999 Lectures series “ Molecular Medicine and Tumours” , Institute of Pathology, Charite’, Humboldt-University Berlin
since 2001: Supervision of 10 graduate students and projects, at the Institute for Ageing and Health, Newcastle University, supervision of 5 masters projects

• Saretzki G, Von Zglinicki T. Replicative aging, telomeres, and oxidative stress. Annals of the New York Academy of Sciences 2002, 959(1), 24-29.
• Saretzki G, Von Zglinicki T. Replicative aging, telomeres, and oxidative stress. In: Increasing Healthy Life Span: Conventional Measures and Slowing the Innate Aging Process. 2002, Vancouver, British Colombia, Canada: New York Academy of Sciences.
• Saretzki G. Telomerase and Oxidative Stress in Embryonic Stem Cells. In: Atwood, C, ed. Embryonic Stem Cells: The Hormonal Regulation of Pluripotency and Embryogenesis. Rijeka, Croatia: InTech, 2011, pp.357-362.
• Wang CF, Maddick M, Miwa S, Jurk D, Czapiewski R, Saretzki G, Langie SAS, Godschalk RWL, Cameron K, von Zglinicki T. Adult-onset, short-term dietary restriction reduces cell senescence in mice. Aging 2010, 2(9), 555-566.
• Passos JF, Nelson G, Wang CF, Richter T, Simillion C, Proctor CJ, Miwa S, Olijslagers S, Hallinan J, Wipat A, Saretzki G, Rudolph KL, Kirkwood TBL, von Zglinicki T. Feedback between p21 and reactive oxygen production is necessary for cell senescence. Molecular Systems Biology 2010, 6, 347.
• Daniels DJ, Clothier C, Swan DC, Saretzki G. Immediate and gradual gene expression changes in telomerase over-expressing fibroblasts. Biochemical and Biophysical Research Communications 2010, 399(1), 7-13.
• Saretzki G. Telomerase beyond telomeres: New roles for an Old Enzyme. In: Gagnon, AN, ed. Telomerase: Composition, Functions and Clinical Implications (DNA: Properties and Modifications, Functions and Interactions, Recombination and Applications. Nova Science Publishers, Inc, 2011, pp.1-34.
• Tuppen HA, Hogan VE, He L, Blakely EL, Worgan L, Al-Dosary M, Saretzki G, Alston CL, Morris AA, Clarke M, Jones S, Devlin AM, Mansour S, Chrzanowska-Lightowlers ZMA, Thorburn DR, McFarland R, Taylor RW. The p.M292T NDUFS2 mutation causes complex I-deficient Leigh syndrome in multiple families. Brain 2010, 133(10), 2952-2963.
• Armstrong L, Tilgner K, Saretzki G, Atkinson SP, Stojkovic M, Moreno R, Przyborski S, Lako M. Human Induced Pluripotent Stem Cell Lines Show Stress Defense Mechanisms and Mitochondrial Regulation Similar to Those of Human Embryonic Stem Cells. Stem Cells 2010, 28(4), 661-673.
• Saretzki G. Cellular senescence in the development and treatment of cancer. Current Pharmaceutical Design 2010, 16(1), 79-100.
• Büchner N, Altschmied J, Jakob S, Saretzki G, Haendeler J. Well-known signaling proteins exert new functions in the nucleus and mitochondria. Antioxidants and Redox Signaling 2010, 13(4), 551-558.
• Saretzki G. Telomerase, mitochondria and oxidative stress. Experimental Gerontology 2009, 44(8), 485-492.
• Yang C, Przyborski S, Cooke MJ, Zhang X, Stewart R, Anyfantis G, Atkinson SP, Saretzki GC, Armstrong L, Lako M. A key role for telomerase reverse transcriptase unit in modulating human embryonic stem cell proliferation, cell cycle dynamics, and in vitro differentiation. Stem Cells 2008, 26(4), 850-863.
• Parker SM, Goriwiec MR, Borthwick LA, Johnson G, Ward C, Lordan JL, Corris PA, Saretzki GC, Fisher AJ. Airway epithelial cell senescence in the lung allograft. American Journal of Transplantation 2008, 8(7), 1544-1549.
• Saretzki G, Walter T, Atkinson S, Passos JF, Bareth B, Keith WN, Stewart R, Hoare S, Stojkovic M, Armstrong L, Von Zglinicki T, Lako M. Downregulation of multiple stress defense mechanisms during differentiation of human embryonic stem cells. Stem Cells 2008, 26(2), 455-464.
• Hapangama DK, Turner MA, Drury JA, Quenby S, Saretzki G, Martin-Ruiz C, Von Zglinicki T. Endometriosis is associated with aberrant endometrial expression of telomerase and increased telomere length. Human Reproduction 2008, 23(7), 1511-1519.
• Ahmed S, Passos JF, Birket MJ, Beckmann T, Brings S, Peters HH, Birch-Machin MA, von Zglinicki TW, Saretzki GC. Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress. Journal of Cell Science 2008, 121(7), 1046-1053.
• Passos J, Saretzki G, von Zglinicki T. DNA damage in telomeres and mitochondria during cellular senescence: Is there a connection?. Nucleic Acids Research 2007, 35(22), 7505-7513.
• Serakinci N, Christensen R, Graakjaer J, Cairney CJ, Keith WN, Alsner J, Saretzki G, Kolvraa S. Ectopically hTERT expressing adult human mesenchymal stem cells are less radiosensitive than their telomerase negative counterpart. Experimental Cell Research 2007, 313(5), 1056-1067.
• Passos JF, Saretzki G, Ahmed S, Nelson G, Richter T, Peters H, Wappler I, Birket MJ, Harold G, Schaeuble K, Birch-Machin MA, Kirkwood TBL, von Zglinicki T. Mitochondrial dysfunction accounts for the stochastic heterogeneity in telomere-dependent senescence. PLoS Biology 2007, 5(5), 1138-1151.
• Ksiazek K, Passos JF, Olijslagers S, Saretzki G, Martin-Ruiz C, von Zglinicki T. Premature senescence of mesothelial cells is associated with non-telomeric DNA damage. Biochemical and Biophysical Research Communications 2007, 362(3), 707-711.
• Richter T, Saretzki G, Nelson G, Melcher M, Olijslagers S, von Zglinicki T. TRF2 overexpression diminishes repair of telomeric single-strand breaks and accelerates telomere shortening in human fibroblasts. Mechanisms of Ageing and Development 2007, 128(4), 340-345.
• Passos JF, Von Zglinicki T, Saretzki G. Mitochondrial dysfunction and cell senescence: Cause or consequence?. In: 2nd Conference on Strategies for Engineered Negligible Senescence (SENS). 2006, Cambridge: Rejuvenation Research, Mary Ann Liebert, Inc. Publishers.
• Jeyapalan J, Saretzki G, Leake A, Tilby MJ, VonZglinicki TW. Neuroblastoma cell apoptosis induction after cisplatin treatment is not telomere–dependent. International Journal of Cancer 2006, 118(11), 2727-34.
• Jeyapalan, J., Saretzki, G., Leake, A., Tilby, M.J., Von Zglinicki, T. Tumour-cell apoptosis after cisplatin treatment is not telomere dependent. International Journal of Cancer 2006, 118(11), 2727-2734.
• Michailidis G, Saretzki G, Hall J. Endogenous and ectopic expression of telomere regulating genes in chicken embryonic fibroblasts. Biochemical and Biophysical Research Communications 2005, 335(1), 240-246.
• von Zglinicki T, Saretzki G, Ladhoff J, di Fagagna FD, Jackson SP. Human cell senescence as a DNA damage response. In: Mechanisms of Ageing and Development: 2nd International Conference on Functional Genomics of Ageing. 2005, Hersonissos, Greece: Elsevier Ireland Ltd.
• Armstrong L, Saretzki G, Peters H, Wappler I, Evans J, Hole N, Von Zglinicki T, Lako M. Overexpression of telomerase confers growth advantage, stress resistance, and enhanced differentiation of ESCs toward the hematopoietic lineage. Stem Cells 2005, 23(4), 516-529.
• Von Zglinicki T, Martin-Ruiz CM, Saretzki G. Telomeres, cell senescence and human ageing. Signal Transduction 2005, 5(3), 103-114.
• Saretzki GC. Telomeres, senescence and longevity: The role of oxidative stress and antioxidants. Current Pharmacogenomics 2005, 3(2), 129-156.
• Lechel A, Satyanarayana A, Ju Z, Plentz RR, Schaetzlein S, Rudolph C, Wilkens L, Wiemann SU, Saretzki G, Malek NP, Manns MP, Buer J, Rudolph KL. The cellular level of telomere dysfunction determines induction of senescence or apoptosis in vivo. EMBO Reports 2005, 6(3), 275-281.
• Martin-Ruiz C, Saretzki G, Petrie J, Ladhoff J, Jeyapalan J, Wei W, Sedivy J, Von Zglinicki T. Stochastic Variation in Telomere Shortening Rate Causes Heterogeneity of Human Fibroblast Replicative Life Span. Journal of Biological Chemistry 2004, 279(17), 17826-17833.
• Armstrong L, Lako M, van Herpe I, Evans J, Saretzki G, Hole N. A role for nucleoprotein Zap3 in the reduction of telomerase activity during embryonic stem cell differentiation. Mechanisms of Development 2004, 121(12), 1509-1522.
• Saretzki G, Armstrong L, Leake A, Lako M, Von Zglinicki T. Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells. Stem Cells 2004, 22(6), 962-971.
• Keys B, Serra V, Saretzki G, von Zglinicki T. Telomere shortening in human fibroblasts is not dependent on the size of the telomeric-3′-overhang. Aging Cell 2004, 3(3), 103-109.
• Jeyapalan, J., Leake, A., Ahmed, S., Saretzki, G., Tilby, M.J., Von Zglinicki, T. The role of telomeres in etoposide induced tumor cell death. Cell Cycle 2004, 3(9), 1169-1176.
• D'Adda Di Fagagna F, Reaper PM, Clay-Farrace L, Fiegler H, Carr P, Von Zglinicki T, Saretzki G, Carter NP, Jackson SP. A DNA damage checkpoint response in telomere-initiated senescence. Nature 2003, 426(6963), 194-198.
• Saretzki G, Serra V, Lorenz M, von Zglinicki T. Extracellular superoxide dismutase is a major antioxidant and slows down telomere shortening in human fibroblasts. In: Free Radical Research: Meeting on Free Radicals and Oxidative Stress. 2003, Ioannina, Greece: Informa Healthcare.
• Serra V, Von Zglinicki T, Lorenz M, Saretzki G. Extracellular superoxide dismutase is a major antioxidant in human fibroblasts and slows telomere shortening. Journal of Biological Chemistry 2003, 278(9), 6824-6830.
• Davies, B.R., Steele, I.A., Edmondson, R.J., Zwolinski, S.A., Saretzki, G., Von Zglinicki, T., O'Hare, M.J. Immortalisation of human ovarian surface epithelium with telomerase and temperature-senstitive SV40 large T antigen. Experimental Cell Research 2003, 288(2), 390-402.
• Saretzki G, Murphy MP, von Zglinicki T. MitoQ counteracts telomere shortening and elongates lifespan of fibroblasts under mild oxidative stress. Aging Cell 2003, 2(2), 141-143.
• Saretzki G, Von Zglinicki T. Telomerase as a promising target for human cancer gene therapy. Drugs of Today 2003, 39(4), 265-276.
• Saretzki G. Telomerase inhibition as cancer therapy. Cancer Letters 2003, 194(2), 209-219.
• Saretzki G, Petersen S, Petersen I, Kolble K, Von Zglinicki T. hTERT gene dosage correlates with telomerase activity in human lung cancer cell lines. Cancer Letters 2002, 176(1), 81-91.
• Lorenz M, Saretzki G, Sitte N, Metzkow S, Von Zglinicki T. BJ fibroblasts display high antioxidant capacity and slow telomere shortening independent of hTERT transfection. Free Radical Biology and Medicine 2001, 31(6), 824-831.
• Saretzki G, Ludwig A, Von Zglinicki T, Runnebaum IB. Ribozyme-mediated telomerase inhibition induces immediate cell loss but not telomere shortening in ovarian cancer cells. Cancer Gene Therapy 2001, 8(10), 827-834.
• Saretzki G, Fischer H, Kaufmann IG, Schewe C, Nadjari B, Blohmer J, Hauptmann S. Telomerase activity in cervical smears. Analytical Cellular Pathology 2001, 23(1), 39-43.
• von Zglinicki T, Serra V, Lorenz M, Saretzki G, Lenzen-Grossimlighaus R, Gessner R, Risch A, Steinhagen-Thiessen E. Short telomeres in patients with vascular dementia: An indicator of low antioxidative capacity and a possible risk factor?. Laboratory Investigation 2000, 80(11), 1739-1747.
• Serra V, Grune T, Sitte N, Saretzki G, Von Zglinicki T. Telomere length as a marker of oxidative stress in primary human fibroblast cultures. Annals of the New York Academy of Sciences 2000, 908, 327-330.
• Pilger R, Sitte N, Saretzki G, von Zglinicki T. Modification of the replicative life-span of human fibroblasts by oxidative stress. Zeitschrift Fur Gerontologie Und Geriatrie 1999, 32, 887.
• Sitte N, Saretzki G, von Zglinicki T. Accelerated telomere shortening in flbroblasts after extended periods of confluency. Free Radical Biology and Medicine 1998, 24, 885-893.
• Mueller JD, Haegle N, Keller G, Mueller E, Saretzky G, Bethke B, Stolte M, Hofler H. Loss of heterozygosity and microsatellite instability in de novo versus ex-adenoma carcinomas of the colorectum. Am J Pathol 1998, 153(6), 1977-84.
• Petersen S, Saretzki G, von Zglinicki T. Preferential accumulation of single-stranded regions in telomeres of human fibroblasts. Experimental Cell Research 1998, 239(1), 152-160.
• Saretzki G, Feng JL, von Zglinicki T, Villeponteau B. Similar gene expression pattern in senescent and hyperoxic- treated fibroblasts. Journals of Gerontology Series a-Biological Sciences and Medical Sciences 1998, 53, B438-B442.
• Saretzki G, Hoffmann U, Rohlke P, Psille R, Gaigal T, Keller G, Hofler H, Loning T, Petersen I, Dietel M. Identification of allelic losses in benign, borderline, and invasive epithelial ovarian tumors and correlation with clinical outcome. Cancer 1997, 80, 1241-1249.
• Reichmuth C, Markus MA, Hillemanns M, Atkinson MJ, Unni KK, Saretzki G, Hofler H. The diagnostic potential of the chromosome translocation t(2;13) in rhabdomyosarcoma: a Pcr study of fresh-frozen and paraffin-embedded tumour samples. Journal of Pathology 1996, 180, 50-7.
• von Zglinicki T, Saretzki G, Docke W, Lotze C. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: a model for senescence?. Experimental Cell Research 1995, 220, 186-193.
• Lawless C, Jurk D, Gillespie CS, Shanley D, Saretzki G, von Zglinicki T, Passos JF. A Stochastic Step Model of Replicative Senescence Explains ROS Production Rate in Ageing Cell Populations. PLoS One 2012, 7(2), e32117.
• Siler U, Jiang Y, Cowley SA, Melguzo D, Tilgner K, Browne C, deWilton A, Pryzborski S, Saretzki G, James WS, Seger RA, Reichenbach J, Lako M, Armstrong L. Derivation and functional analysis of patient specific induced pluripotent stem cells (IPSCS) as an in vitro model of chronic granulomatous disease. In: 15th Biennial Meeting European Society for Immunodeficiency (ESID). 2012, Florence, Italy: Springer.
• Siler U, Jiang Y, Cowley SA, Melguzo D, Tilgner K, Browne C, Dewilton A, Pryzborski S, Saretzki G, James WS, Seger RA, Reichenbach J, Lako M, Armstrong L. Derivation and Functional Analysis of Patient Specific Induced Pluripotent Stem Cells as an In Vitro Model of Chronic Granulomatous Disease. In: 15th Annual Meeting of the American Society of Gene and Cell Therapy (ASGCT). 2012, Philadelphia, Pennsylvania, USA: Nature Publishing Group.
• Gorowiec MR, Borthwick LA, Parker SM, Kirby JA, Saretzki GC, Fisher AJ. Free radical generation induces epithelial-to-mesenchymal transition in lung epithelium via a TGF-beta 1-dependent mechanism. Free Radical Biology & Medicine 2012, 52(6), 1024-1032.
• Tsolou A, Nelson G, Trachana V, Chondrogianni N, Saretzki G, von Zglinicki T, Gonos ES. The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult. IUBMB Life 2012, 64(5), 432-442.
• Ludwig A, Saretzki G, Holm PS, Tiemann F, Lorenz M, Emrich T, Harley CB, von Zglinicki T. Ribozyme cleavage of telomerase mRNA sensitizes breast epithelial cells to inhibitors of topoisomerase. Cancer Research 2001, 61(7), 3053-3061.
• Saretzki G, von Zglinicki T. [Replicative senescence as a model of aging: the role of oxidative stress and telomere shortening--an overview]. Zeitschrift fur Gerontologie und Geriatrie 1999, 32(2), 69-75.
• von Zglinicki T, Ludwig A, Saretzki G. Ribozymes inhibiting the catalytic subunit of human telomerase (hTERT). Germany & International, DE199039615, INT10003356, 1999.
• Saretzki G, Sitte N, Merkel U, Wurm RE, von Zglinicki T. Telomere shortening triggers a p53-dependent cell cycle arrest via accumulation of G-rich single stranded DNA fragments. Oncogene 1999, 18(37), 5148-5158.