Newcastle University

Qualifications

PhD - Newcastle University, UK
BSc(hons) - Hull University, UK

Memberships

Diabetes UK
Biochemical Society
European Association for the Study of Diabetes

Research Interests

My main area of research is aimed at understanding the regulation of glucose metabolism in the pancreatic beta-cell and its relationship to the secretion of insulin by these cells. A significant focus is understanding the cellular regulatory mechanisms of the glucose sensor enzyme glucokinase. Mutations in the glucokinase gene are associated with diabetes (GCK-MODY) and hypoglycaemia. I am using these mutations as a resource to understand the cellular regulation of glucokinase.

Other Expertise

Development of novel methods for detecting protein-protein interactions
Expression of glucokinase in other tissues
Glucose-regulated gene expression

Postgraduate Supervision

I currently co-supervise 3 PhD students and 1 MPhil student.

Esteem Indicators

• Diabetes UK RD Lawrence Fellowship
• EFSD Albert Renold Travel Fellowship
• EMBO Short-Term Travel Fellowship
• Innovators in Diabetes (iDia) programme recipient
  

Funding

I currently recieve funding from:
Diabetes UK
Diabetes Research and Wellness Foundation

• Petrie JL, Al-Oanzi ZH, Arden C, Tudhope SJ, Mann J, Kieswich J, Yaqoob MM, Towle HC, Agius L. Glucose induces protein targeting to glycogen in hepatocytes by fructose 2,6-bisphosphate-mediated recruitment of MondoA to the promoter. Molecular and Cellular Biology 2013, 33(4), 725-738.
• Tudhope SJ, Wang CC, Petrie JL, Potts L, Malcomson F, Kieswich J, Yaqoob MM, Arden C, Hampson LJ, Agius L. A Novel Mechanism for Regulating Hepatic Glycogen Synthesis Involving Serotonin and Cyclin-Dependent Kinase-5. Diabetes 2012, 61(1), 49-60.
• Arden C, Tudhope SJ, Petrie JL, Al-Oanzi ZH, Cullen KS, Lange AJ, Towle HC, Agius L. Fructose 2,6-bisphosphate is essential for glucose-regulated genetranscription of glucose-6-phosphatase and other ChREBP target genes inhepatocytes. Biochemical Journal 2012, 443(1), 111-123.
• Arden C, Petrie JL, Tudhope SJ, Al-Oanzi Z, Claydon AJ, Beynon RJ, Towle HC, Agius L. Elevated glucose represses liver glucokinase and induces its regulatory protein to safeguard hepatic phosphate homeostasis. Diabetes 2011, 60(12), 3110-3120.
• Cullen KS, Matschinsky FM, Agius L, Arden C. Susceptibility of Glucokinase-MODY Mutants to Inactivation by Oxidative Stress in Pancreaticβ-Cells. Diabetes 2011, 60(12), 3175-3185.
• Persaud S, Arden C, Bergsten P, Bone A, Brown J, Dunmore S, Harrison M, Hauge-Evans A, Kelly C, King A, Maffucci T, Marriott C, McClenaghan N, Morgan N, Reers C, Russell M, Turner M, Willoughby E, Younis M, Zhi Z, Jones P. Pseudoislets as primary islet replacements for research: Report on a symposium at King's College London, London UK. Islets 2010, 2(4), 236-239.
• Arden C, Hampson L, Huang G, Shaw J, Aldibbiat A, Holliman G, Manas D, Khan S, Lange A, Agius L. A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic β-cells. Biochemical Journal 2008, 411(1), 41-51.
• Mukhtar MH, Payne VA, Arden C, Harbottle A, Khan S, Lange AJ, Agius L. Inhibition of glucokinase translocation by AMP-activated protein kinase is associated with phosphorylation of both GKRP and 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 2008, 294(3), R766-R774.
• Arden C, Trainer A, De La Iglesia N, Scougall KT, Gloyn AL, Lange AJ, Shaw J, Matschinsky FM, Agius L. Cell biology assessment of glucokinase mutations V62M and G72R in pancreatic β-cells: Evidence for cellular instability of catalytic activity. Diabetes 2007, 56(7), 1773-1782.
• Payne VA, Arden C, Lange AJ, Agius L. Contributions of glucokinase and phosphofructokinase-2/fructose bisphosphatase-2 to the elevated glycolysis in hepatocytes from Zucker fa/fa rats. American Journal of Physiology - Regulatory Integrative and Comparative Physiology 2007, 293(2), R618-R625.
• Hampson LJ, Arden C, Agius L, Ganotidis M, Kosmopoulou MN, Tiraidis C, Elemes Y, Sakarellos C, Leonidas DD, Oikonomakos NG. Bioactivity of glycogen phosphorylase inhibitors that bind to the purine nucleoside site. Bioorganic and Medicinal Chemistry 2006, 14(23), 7835-7845.
• Arden C, Baltrusch S, Agius L. Glucokinase regulatory protein is associated with mitochondria in hepatocytes. FEBS Letters 2006, 580(8), 2065-2070.
• Arden C, Green AR, Hampson LJ, Aiston S, Harndahl L, Greenberg CC, Brady MJ, Freeman S, Poucher SM, Agius L. Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa/fa rats. FEBS Journal 2006, 273(9), 1989-1999.
• Aiston S, Hampson LJ, Arden C, Iynedjian PB, Agius L. The role of protein kinase B/Akt in insulin-induced inactivation of phosphorylase in rat hepatocytes. Diabetologia 2006, 49(1), 174-182.
• Payne VA, Arden C, Wu C, Lange AJ, Agius L. Dual role of phosphofructokinase-2/fructose bisphosphatase-2 in regulating the compartmentation and expression of glucokinase in hepatocytes. Diabetes 2005, 54(7), 1949-1957.
• Arden C, Harboottle A, Baltrusch S, Tiedge M, Agius L. Glucokinase is an integral component of the insulin granules in glucose-responsive insulin secretory cells and does not translocate during glucose stimulation. Diabetes 2004, 53(9), 2346-2352.