Publication:

Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis (1988)

Author(s): S. P. Fussey;J. R. Guest;O. F. James;M. F. Bassendine;S. J. Yeaman

  • : Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis

Abstract: Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease characterized by the presence of antimitochondrial antibodies in the serum. It is possible that the PBC-specific immunoreactive trypsin-sensitive antigens on the inner mitochondrial membrane, termed M2, are important in the pathogenesis of this autoimmune disease. We have previously shown that a major M2"a" antigen is the E2 component of the pyruvate dehydrogenase multienzyme complex located within mitochondria. Analysis of the primary structure of the E2 components of all three 2-oxo acid dehydrogenase complexes reveals a high degree of homology with a similar highly segmented structure including lipoyl domains, E3-binding domains, C-terminal catalytic domains, and interdomain linker sequences. Immunoblotting of PBC patients' sera against purified E2 protein from 2-oxoglutarate dehydrogenase complex and branched-chain 2-oxo acid dehydrogenase complex reveals that these polypeptides are also autoantigens in this disease. Sera from 29 of 40 (72.5%) PBC patients gave a positive response against bovine 2-oxoglutarate dehydrogenase complex E2 and from 25 of 40 (62.5%) PBC patients gave a positive response against bovine branched-chain 2-oxo acid dehydrogenase complex E2. All 40 PBC patients (100%) have autoantibodies directed against at least one of the E2 components of the family of 2-oxo acid dehydrogenase complexes. Identification of these M2 mitochondrial autoantigens and detailed knowledge of their structure will allow important questions concerning this autoimmune disease to be addressed.

Notes: 0027-8424 Journal Article

  • Short Title: Identification and analysis of the major M2 autoantigens in primary biliary cirrhosis
  • Date: Nov
  • Journal: Proc Natl Acad Sci U S A
  • Volume: 85
  • Issue: 22
  • Pages: 8654-8
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Acetyltransferases/*immunology Amino Acid Sequence Animals Autoantigens/*analysis Cattle Humans Immunoblotting Intracellular Membranes/enzymology/*immunology Liver Cirrhosis, Biliary/enzymology/*immunology Mitochondria, Liver/enzymology/*immunology Molecular Sequence Data Pyruvate Dehydrogenase Complex/*immunology Research Support, Non-U.S. Gov't