Guidelines for Submission of Data
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Guidelines for Submission of Data |
As part of our own on-going gene expression studies, we will be adding specific gene expression data into the database ourselves, however, we will actively encourage other research groups to enter their own data into the database.
Data can be submitted to the EADHB database in the following ways:
A) By e-mailing/posting images of in-situ hybridisation data together with the accompanying information, (contact details, probe sequence and experimental conditions used), to the address found on the contacts page. We will enter the data into the database ourselves by spatially mapping the data and contact you once it is finished before uploading the data to the publicly accessible database.
B) You can also spatially map the data yourself by using MAPaint and add the data to a locally created EADHB database which can be done with the EADHB database software available from the Downloads page. The final entries can then be electronically submitted to the central database (more than one entry can be submitted at once) and after further editorial checking, this data will then be moved into the Public Database for other people to access.
If you are interested in mapping your own data, we will send you a CD containing the MAPaint software free of charge. Please note that the MAPaint program will only run on a UNIX based operating system (eg Linux, UNIX, MacOSX) but will not run on Windows or MacOS9 or earlier.Instructions on how to use the MAPaint software can be found on the Edinburgh Mouse Atlas software pages here. These instructions will be replaced with a more pertinent copy related to the EADHB database specifically when they become available. The Help menu of the EADHB database contains instructions on how to use the EADHB software to make local databases, create entries and submit data to the Central Database.
If you require more information please e-mail us or contact us via: EADHB Database Office, Institute of Human Genetics, International Centre for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ, UK.
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Very Good Quality The section to the left is a good example of the optimum data to be used for mapping purposes. This is immunocytochemistry data which can be mapped as well as tissue in situ hybridisation data. The section is clean and in very good condition, the illumination is even and bright while the staining is in stark contrast to the background enabling the patterning to be distinguished easily. |
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Very Good Quality The radioactive in-situ data to the left is also a good example of ideal data. The section is completely intact while the slide is clearly devoid of debris and artefact. The staining is very strong and easily discernable from the background making the mapping process easier. |
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Sufficient Quality The tissue in situ hybridisation data in this image is of acceptable quality to map into the database. The section is clean and there is sufficient contrast between the staining and the background although it is not as pronounced as in the previous sections. The background is also darker which will make it harder to match the section in the 3D standard model. |
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Sufficient Quality This section is of a non-radioactive TISH experiment. It is also acceptable staining but like the previous section the background is slightly lighter than it ideally should be, although the staining is strong and easily seen against the background. |
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Unsuitable Section Data This section is of a radioactive in situ but unlike the previous
sections is not useable for mapping. The section is folded over on itself
and so will not be able to be mapped effectively. The folds in the tissue
appear lighter than other areas and so may be incorrectly shown in the
mapping process as signal data. The background is also quite dark which
would make it harder to match sections to the 3D standard model. |