Microarray Facility
Facility Technician: Fangli Chen Bsc
Expression profiling is an important technology for the identification
of novel genetic pathways involved in development and disease. Under the academic
lead of Dr Heiko Peters, the Affymetrix-based platform housed at the Institute of Genetic Medicine provides a service for genome-wide expression analyses of
human RNA samples, but also for a wide range of model organisms, including yeast,
zebrafish, rat and mouse. For low RNA quantities the Agilent Bioanalyser, a
microfluidics-based platform is available to assess RNA quality. Linear amplification
is routinely carried out to enable robust gene expression profiling using 25-50ng
of total RNA as starting material. A recent upgrading enabled the facility to
run high-density expression arrays for the detection of alternatively spliced
transcripts, and mapping arrays to perform linkage analysis, association, and
copy number studies.
