Cited2 controls left-right patterning and heart development through a Nodal-Pitx2c pathway (2004)

Author(s): Bamforth SD, Braganca J, Farthing CR, Schneider JE, Broadbent C, Michell AC, Clarke K, Neubauer S, Norris D, Brown NA, Anderson RH, Bhattacharya S

    Abstract: Malformations of the septum, outflow tract and aortic arch are the most common congenital cardiovascular defects and occur in mice lacking Cited2, a transcriptional coactivator of TFAP2. Here we show that Cited2(-/-) mice also develop laterality defects, including right isomerism, abnormal cardiac looping and hyposplenia, which are suppressed on a mixed genetic background. Cited2(-/-) mice lack expression of the Nodal target genes Pitx2c, Nodal and Ebaf in the left lateral plate mesoderm, where they are required for establishing laterality and cardiovascular development. CITED2 and TFAP2 were detected at the Pitx2c promoter in embryonic hearts, and they activate Pitx2c transcription in transient transfection assays. We propose that an abnormal Nodal-Pitx2c pathway represents a unifying mechanism for the cardiovascular malformations observed in Cited2(-/-) mice, and that such malformations may be the sole manifestation of a laterality defect.

      • Date: 01-11-2004
      • Journal: Nature Genetics
      • Volume: 36
      • Issue: 11
      • Pages: 1189-1196
      • Publisher: Nature Publishing Group
      • Publication type: Article
      • Bibliographic status: Published

      Keywords: Animals *Body Patterning Crosses, Genetic DNA-Binding Proteins/genetics/*physiology Gene Expression Heart/*embryology Heart Defects, Congenital/genetics Homeodomain Proteins/genetics Mice Mice, Inbred C57BL Mice, Knockout Nuclear Proteins/genetics Organogenesis Repressor Proteins/*physiology Research Support, Non-U.S. Gov't Trans-Activators/*physiology Transcription Factor AP-2 Transcription Factors/genetics Transforming Growth Factor beta/*genetics


      Dr Simon Bamforth