Dr Annette Meeson
- Email: firstname.lastname@example.org
- Telephone: +44 (0) 191 241 8856
- Address: Institute of Genetic Medicine
International Centre for Life
Newcastle upon Tyne
Telephone: +44 (0) 191 241 8856
Address: Institute of Genetic Medicine
International Centre for Life
Newcastle upon Tyne
2001- 2005 Instructor, Molecular Cardiology, UT Southwestern Medical Ctr,
1997- 2001 Postdoctoral Fellow, Molecular Cardiology, UT Southwestern
Medical Ctr, Dallas, TX
1994-1997 Postdoctoral Fellow, Skirball Institute, New York University Medical
Ctr, New York, NY
1990-1994 PhD, Neurobiology, Imperial College of Science, Technology and Medicine,
1987-1990 Scientific Officer, National Institute of Medical Research, Mill Hill,
Research Fellow, Advanced Medical and Dental Institute, Universiti Sains, Malaysia, 1st January 2016 -Jan 2017
Research Fellow, Advanced Medical and Dental Institute, Universiti Sains, Malaysia, January 2017 - January 2018
Adult stem cells
Although many cells are fate committed before or shortly after birth some adult tissues retain stem cells that are capable of multilineage differentiation. Our research efforts focus on expanding our knowledge of the fundamental regulatory mechanisms that restrict differentiation of these cells during normal development and that regulate the cellular proliferation, differentiation and plasticity of these cells in response to injury or disease.
Stem cells and cardiac regeneration.
Recent studies suggest that the heart is capable of limited regeneration which may be due to the activation of a resident cardiac stem cell population or to the recruitment of stem cells from other tissues. We have identified and isolated stem cells known as side population (SP) cells from both human heart and bone marrow. SP cells from both these sources are capable of migration into the injured myocardium and are able to differentiation towards a cardiac lineage.
Stem cells, tumour formation and cancers.
There is an increasing body of evidence that suggests that recurrence of some cancers may be due in part to cancer stem cell like cells. Stem cells make ideal candidates for the progression of cancer as they are long lived, slow cycling and exhibit some level of drug resistance.
Breast cancer: In collaboration with surgical teams at the Royal Victoria Infirmary we have identified two putative breast cancer stem cell populations CD44+/CD24- breast cancer stem cells and breast cancer SP cells. We have been able to identify SP cells in solid breast tumours, from fluid obtained as fine needle aspirates (FNAs) associated with palpable breast tumours and in breast cancer cell lines. More importantly, we have shown that these cells are more resistant than the bulk tumour cells to drugs currently used in the treatment of breast cancer patients. Our recent analysis of FNAs suggests that the presence of SP cells is more frequently associated with triple negative cancers (associated with patients whose prognostic outcome is generally poor) and that a statistically significant relationship exists between the presence of SP cells and ER- breast tumours.
Ovarian cancer: We have also been able to identify SP cells in ascitic fluid of ovarian cancer patients and again can shown that these cells are capable of drug resistance to currently used chemotherapeutic agents.
Parathyroid stem cells – Recent studies have suggested that benign tumour growth might also be in part due to proliferation of stem cells. In patients with hyperparathyroidism, a hyperplastic response leads to abnormal growth of one or more of the parathyroid glands. However, the mechanisms behind this remain unclear. We have begun to examine these glands for the presence of putative stem cells populations
Programme leader for Stem cells and regenerative medicine MRes 2007 to present day
Module leader for Stem cells and regenerative medicine MRes 2007 to present day
Deputy Degree Program Director Masters of Clinical Research 2016 -2017
- Shaharuddin B, Ahmad S, MdLatar N, Ali S, Meeson A. A Human Corneal Epithelial Cell Line Model for LimbalStem Cell Biology and Limbal Immunobiology. Stem Cells Translational Medicine 2016, (ePub ahead of Print).
- Shaharuddin B, Osei-Bempong C, Ahmad S, Rooney P, Ali S, Oldershaw R, Meeson A. Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane. Regenerative Medicine 2016, 11(3), 273-286.
- Md Latar N, Mahkamova K, Aspinall S, Meeson A. Thyroid Stem Cells: Concept and Clinical Implications. Journal of Surgical Academia 2016, 6(2), 4-11.
- Shaharuddin B, Meeson A. Current Perspectives on Tissue Engineering for the Management of Limbal Stem Cell Deficiency. Journal of Stem Cells Research, Reviews & Reports 2015, 2(1), 1-8.
- Douglass A, Meeson AP, Overbeck-Zubrzycka D, Brain JG, Bennett MR, Lamb CA, Lennard TWJ, Browell D, Ali S, Kirby JA. Breast cancer metastasis: demonstration that FOXP3 regulates CXCR4 expression and the response to CXCL12. The Journal of Pathology 2014, 234(1), 74-85.
- Meeson A, Weisel RD. Cardiac cell therapy to restore contracting elements. In: Li,R-E; Weisel,RD, ed. Cardiac Regeneration and Repair. Woodhead Publishing, 2014.
- Shaharuddin B, Harvey I, Ahmad S, Ali S, Meeson A. Characterisation of Human Limbal Side Population Cells Isolated Using an Optimised Protocol From an Immortalised Epithelial Cell Line and Primary Limbal Cultures. Stem Cell Reviews and Reports 2014, 10(2), 240-250.
- Eyre R, Harvey I, Stemke-Hale K, Lennard TWJ, Tyson-Capper AJ, Meeson AP. Reversing paclitaxel resistance in ovarian cancer cells via inhibition of the ABCB1 expressing side population. Tumor Biology 2014.
- Meeson A. Cardiac Stem cells. European Pharmaceutical Review 2013, 18(2), 29-31.
- Mallini P, Lennard T, Kirby J, Meeson A. Epithelial-to-mesenchymal transition: What is the impact on breast cancer stem cells and drug resistance. Cancer Treatment Reviews 2013, 40(3), 341-348.
- Shaharuddin B, Ahmad S, Meeson A, Ali S. Immunological Properties of Ocular Surface and Importance of Limbal Stem Cells for Transplantation. Stem Cells Translational Medicine 2013, 2(8), 614-624.
- Shaharuddin B, Ahmad S, Ali S, Meeson A. Limbal Side Population cells: a future treatment for limbal stem cell deficiency. Regenerative Medicine 2013, 8(3), 319-331.
- Knuth CA, Clark ME, Meeson AP, Khan SK, Dowen DJ, Deehan DJ, Oldershaw RA. Low Oxygen Tension is Critical for the Culture Of Human Mesenchymal Stem Cells with Strong Osteogenic Potential from Haemarthrosis Fluid. Stem Cell Reviews and Reports 2013, 9(5), 599-608.
- Meeson A, Fuller A, Breault D, Owens W, Richardson G. Optimised Protocols for the Identification of the Murine Cardiac Side Population. Stem Cell Reviews and Reports 2013, 9(5), 731-739.
- Britton KM, Eyre R, Harvey IJ, Stemke-Hale K, Browell D, Lennard TWJ, Meeson AP. Breast cancer, side population cells and ABCG2 expression. Cancer Letters 2012, 323(1), 97-105.
- Meeson A. Side Population Cells. In: Schwab, M, ed. Encyclopedia of Cancer. Berlin, Germany: Springer, 2012.
- Douglass S, Ali S, Meeson AP, Browell D, Kirby JA. The role of FOXP3 in the development and metastatic spread of breast cancer. Cancer and Metastasis Reviews 2012, 31(3-4), 843-854.
- Alfakir A, Dawe N, Eyre R, Tyson-Capper A, Britton K, Robson SC, Meeson AP. The temporal and spatial expression patterns of ABCG2 in the developing human heart. International Journal of Cardiology 2012, 156(2), 133-138.
- Britton KM, Kirby JA, Lennard TWJ, Meeson AP. Cancer Stem Cells and Side Population Cells in Breast Cancer and Metastasis. Cancers 2011, 3(2), 2106-2130.
- Meeson A. Islet-1 expressing cells in cardiac development, where does it end?. Stem Cells and Development 2011, 20(9), 1477.
- Nilsson J, Ali S, Harvey I, Kirby J, Meeson A. Stem cell therapy: a role for CXCR4 in homing bone marrow side population cells to areas of myocardial damage. International Journal of Cardiology 2010, 145(3), 554-555.
- Naseem RH, Meeson AP, DiMaio JM, White MD, Kallhoff J, Humphries C, Goetsch SG, De Windt LJ, Williams MA, Garry MG, Garry DJ. Reparative myocardial mechanisms in adult C57Bl/6 and MRL mice following injury. Physiological Genomics 2007, 30(1), 44-52.
- Meeson AP, Shi X, Alexander MS, Williams RS, Allen RE, Jiang N, Adham IM, Goetsch SC, Hammer RE, Garry DJ. Sox15 and Fhl3 transcriptionally coactivate Foxk1 and regulate myogenic progenitor cells. EMBO Journal 2007, 26(7), 1902-1912.
- Meeson AP, Hawke TJ, Graham S, Jiang N, Elterman J, Hutcheson K, DiMaio J. Michael, Gallardo TD, Garry DJ. Cellular and Molecular Regulation of Skeletal Muscle Side Population Cells. Stem Cells 2004, 22(7), 1305-1320.
- Martin CM, Meeson AP, Robertson SC, Hawke TJ, Richardson JA, Bates S, Goetsch SC, Gallardo TD, Garry DJ. Persistent expression of the ATP-binding cassette transporter, Abcg2, identifies cardiac SP cells in the developing and adult heart. Developmental Biology 2004, 265(1), 262-275.
- Hawke TJ, Meeson A, Jiang N, Shalley S, Hutcheson K, Di Maio JM, Garry DJ. p21 is essential for normal myogenic progenitor cell function in regenerating skeletal muscle. AJP: Cell Physiology Online 2003, 285(5), C1019-C1027.
- Garry DJ, Masino AM, Meeson AP, Martin CM. Stem cell biology and therapeutic applications. Current opinion in Nephrology and Hypertension 2003, 12(4), 447-454.
- Meeson AP, Radford N, Shelton J, Pradeep PA, Mammen P, Michael DiMaio J, Hutcheson K, Kong Y, Elterman J, Sanders Williams R, Garry DJ. Adaptive Mechanisms That Preserve Cardiac Function in Mice Without Myoglobin. Circulation Research 2001, 88(7), 713-720.
- Grange RW, Meeson AP, Chin E, Lau KS, Stull JT, Shelton JM, Williams RS, Garry DJ. Functional and molecular adaptations in skeletal muscle of myoglobin mutant mice. American Journal of Physiology: Cell Physiology 2001, 281(5), 1487-1494.
- Garry DJ, Meeson A, Yang Z, Williams RS. Life without myoglobin. Cellular and Molecular Life Sciences 2000, 57(6), 896-898.
- Garry DJ, Meeson A, Elterman J, Zhao Y, Yang P, Bassel-Duby R, Williams RS. Myogenic stem cell function is impaired in mice lacking the forkhead/winged helix protein MNF. Proceedings of the National Academy of Sciences 2000, 97(10), 5416-5426.
- Meeson A, Agrilla M, Neufeld G, Lang R. VEGF deprivation-induced apoptosis is an essential component of programmed capillary regression. Development 1999, 126(7), 1407-1415.
- A.P.Meeson, M. Palmer, M. Calfon and R. Lang. A relationship between apoptosis and flow during programmed capillary regression is revealed by vital analysis. Development 1996, 122, 3929-3938.
- R. Reynolds, I. Cenci di Bello, A. Meeson and S. Piddlesden. Comparison of a chemically mediated and an immunologically mediated demyelinating lesion model. Methods: A companion to Methods in Enzymology 1996, 10, 440-452.
- A.P. Meeson, S. Piddlesden, B.P. Morgan and R. Reynolds. The distribution of inflammatory demyelinating lesions in the CNS of rats with antibody-augmented demyelinating experimental allergic encephalomyelitis (ADEAE. Experimental Neurology 1994, 129, 2-13.
- J. Newcombe, A.P. Meeson and M. L. Cuzner. Immunocytochemical characterization of primary glial cell cultures form normal adult human brain. Neuropathology & Applied Neurobiology 1988, 14, 453-465.