Institute of Neuroscience

Biomarker Assay Service

Biomarker Assay Service

An experienced laboratory offering a wide range of procedures to process clinical research samples.

We are a NIHR Biomedical Research Centre/Biomedical Research Unit funded lab regularly involved in clinical ageing research. Our services range from handling of blood samples and generation of suitable conditioned material and aliquots for further analysis. To more specific assays such as: analysis of white blood cells, DNA damage and repair capability, telomere length measurements by qPCR and ApoE genotyping. We also offer a wide variety of biomarker assays related to heart failure, inflammation and insulin-like growth factor binding proteins.

Our set-up allows for the processing of large numbers of samples on a regular basis which often fits the needs of clinical research projects with a constant recruitment of participants and collection of samples. This 'intermediate' capability has made our team and our facility suitable for several research projects, including MRC and BBSRC funded projects such as the Newcastle 85+ study, Newcastle Thousand Families and HALCyon and enabled the involvement of the facility in collaborative research with other national and international Universities (Boston, USA; Leiden, Netherlands; Keio University, Tokyo, Japan; InChianti Study, Parma University, Italy) and with industrial partners (Unilever, Procter and Gamble).

We offer a wide range of procedures with very flexible costs according to the magnitude of the study. Beyond those assays already available in our lab, we can also assist researchers with the development of reliable assays for new biomarkers and overall costings for grant applications.

Routine procedures include:

  • Processing of blood samples
  • Isolation of Cells from Blood Sample
  • Cryogenic conditioning of blood cell pellets
  • DNA isolation
  • RNA isolation
  • Measurement of Telomere length by qPCR
  • Genotyping of Mice from Ear Notches
  • Genotyping of DNA samples
  • Analysis of RNA expression by RT qPCR
  • Plasma/Serum Biomarker assessment by Sandwich ELISA
  • Analysis of cultured cells DNA damage and repair

For further details and costs of analysis, please contact Dr. Carmen Martin-Ruiz