Publication:
Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase (1993)
Author(s): J. E. Sadlock;R. N. Lightowlers;R. A. Capaldi;E. A. Schon
- : Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase
Abstract: Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.
Notes: 0006-3002 Journal Article
- Short Title: Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase
- Date: Feb 20
- Journal: Biochim Biophys Acta
- Volume: 1172
- Issue: 1-2
- Pages: 223-5
- Publication type: Article
- Bibliographic status: Published
- Author Address: Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8382530
Keywords: Amino Acid Sequence Base Sequence Comparative Study DNA, Mitochondrial/*genetics/isolation & purification Electron Transport Complex IV/*genetics Humans Macromolecular Substances Molecular Sequence Data RNA, Messenger/genetics/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Sequence Homology, Nucleic Acid
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Professor Robert Lightowlers
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