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Publication:

Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase (1993)

Author(s): J. E. Sadlock;R. N. Lightowlers;R. A. Capaldi;E. A. Schon

  • : Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase

Abstract: Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.

Notes: 0006-3002 Journal Article

  • Short Title: Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase
  • Date: Feb 20
  • Journal: Biochim Biophys Acta
  • Volume: 1172
  • Issue: 1-2
  • Pages: 223-5
  • Publication type: Article
  • Bibliographic status: Published

Keywords: Amino Acid Sequence Base Sequence Comparative Study DNA, Mitochondrial/*genetics/isolation & purification Electron Transport Complex IV/*genetics Humans Macromolecular Substances Molecular Sequence Data RNA, Messenger/genetics/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Sequence Homology, Nucleic Acid

Staff

Professor Robert Lightowlers
Director, ICaMB and Professor of Molecular Neuroscience