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Modules

Modules

BGM2061 : Protein Trafficking and Biological Membranes

Semesters
Semester 2 Credit Value: 20
ECTS Credits: 10.0

Aims

1. To introduce the concept of compartmentalisation within eukaryotic cells.
2. To illustrate common and recurrent themes by which proteins are delivered to their correct cellular
address.
3. To introduce glucose transporters as an example of protein trafficking.
4. To provide examples of post-translational modifications in cellular trafficking.
5. To reveal the dynamic nature of membrane lipid and protein biochemistry and how it relates to cellular processes.
6. To examine the synthesis and topogenesis of membrane proteins.
7. To provide opportunities for discussion of research publications
8. To reveal the dynamic nature of membrane lipid and protein biochemistry and how it relates to cellular processes.
9. To provide an understanding of key aspects of membrane structure and the physical contraints of the membrane environment.
10. To examine the synthesis and topogenesis of membrane proteins.
11. To provide opportunities for discussion of research publications

Outline Of Syllabus

Biological membranes are critical for maintaining cellular function, but at the same time they impede the movement of molecules and proteins within the cell. The module will consider:

biological membranes, looking at micelles and bilayered liposomes, and detergents;
recognition of membrane proteins from their polypeptide sequences;
how membrane proteins are synthesized and end up where they should go;
different types of membrane, e.g. viral, bacterial, archeal, sub-cellular and cellular;
the concepts of compartmentalisation within eukaryotic cells with emphasis on some of the common and recurrent
themes by which proteins are delivered to their correct cellular location;
role of molecular chaperones in protein trafficking and folding;
the sorting and recycling of proteins;
post-translational modifications of proteins.

Teaching Methods

Teaching Activities
Category Activity Number Length Student Hours Comment
Scheduled Learning And Teaching ActivitiesLecture201:0020:00N/A
Scheduled Learning And Teaching ActivitiesPractical26:0012:00N/A
Guided Independent StudyReflective learning activity21:002:002 Question and Answer and Feedback sessions
Guided Independent StudyIndependent study1166:00166:00N/A
Total200:00
Teaching Rationale And Relationship

The lectures will introduce and build upon previous exposure to the concepts in the learning outcomes. Some of the concepts and ideas covered in the lectures will be re-enforced in practical sessions which will give the students ‘handson’ experience of purifying proteins. One of the feedback sessions will be used to discuss general concepts of the module, and to review the material covered in the lab (lab recording and reporting skills). The second feedback session will be used to discuss the timed essay, and review essay exam skills.

Assessment Methods

The format of resits will be determined by the Board of Examiners

Exams
Description Length Semester When Set Percentage Comment
Written Examination1202A80EMI (30%): 30 minutes. Essay x 2 (70%) : 90 minutes.
Other Assessment
Description Semester When Set Percentage Comment
Essay2M10Timed essay.
Practical/lab report2M10Practical Writeup
Assessment Rationale And Relationship

Exams test the knowledge across the module.
Practicals assess students understanding of key points in the lectures, ability to report and analyse results, and also their practical and team working skills (students will be working in groups of 2 or more for the practical).
The timed essay will examine the students’ ability to produce an essay under exam conditions on the subject area of the module.

NJM: Purification of proteins by affinity chromatography using two different strategies (12 hours)

Reading Lists

Timetable