Paul O'Gorman Building
This building is named after Paul O'Gorman, who died from leukaemia at the age of 15.
Funding and sponsors
Children with Cancer UK was founded by Eddie and Marion O’Gorman, who lost their children Jean and Paul to cancer in the same year. The building is named in Paul’s memory.
This purpose built research building was completed in 2004, costing a total of £10m, and was opened in 2005 by the late Sir Bobby Robson.
Main focus of work in the building
Research at the Paul O’Gorman is mostly centred around biological studies. Researchers here aim to identify molecular and biological differences between normal and tumour cells. This can then be exploited to design new anti-cancer drugs which can be tested in clinical trials.
The facility now houses around 200 research staff.
The equipment in our building includes:
- Q-PCR and Digital-PCR
- Specialist and Laser-Capture Microscopy
- Cell Irradiator
- Protein production
Chromatography and Mass spectrometryChromatography and Mass spectrometry
The concentration of a wide range of anti-cancer agents present in patient samples can be measured using this facility. This allows us to determine if the optimal therapeutic dose of anti-cancer drugs has been achieved.
Analysis is performed on a range of sample types including plasma, dry blood spots, tumour and tissue homogenate.
There are more than 90 validated analytical methods utilising:
- two AB-SCIEX API4000 Triple-Quadrupole Mass spectrometers,
- one AB-SCIEX 3200QTRAP Mass spectrometer
- four Waters Alliance HPLC’s with fluorescence, radiochemical and ultraviolet detection
- a robotic solid-phase extraction for sample preparation
For more information please contact Phil Berry (firstname.lastname@example.org)
Authenticated cell bankAuthenticated cell bank
Many research publications now require Certificates of Authenticity for any cell lines used in the research, before publication of any associated data.
The authenticated cell bank held in the Paul O’Gorman building provides:
- over 200 authenticated cell lines of various tumour cell types, housed in a large liquid nitrogen refrigerator
- controlled access to the bank ensures cell stocks are maintained
- full record keeping, reports and traceability of cell lines
For more information please contact Claire Hutton (email@example.com)
ImageStream and Fluorescent Activated Cell SortingImageStream and Fluorescent Activated Cell Sorting
Imaging and standard flow cytometry are principally used to detect numerous characteristics of normal and cancer cells. These include biomarkers relevant to prognosis or response to specific drugs. They are also used to monitor residual cancer following chemotherapy in clinical samples.
The following equipment is available:
- Amnis ImagestreamX MkII
- BD FACSCalibur (blue and red lasers)
- BD FACSCanto II (blue, red and violet lasers)
- BD Aria II fitted with blue and red lasers (upgrade to 4 lasers pending)
For more information please contact David Jamieson (firstname.lastname@example.org)
The Aperio Virtual Pathology SystemThe Aperio Virtual Pathology System
The Aperio is a slide scanner which allows you to image Immunohistochemistry slides, enabling you to view your images virtually. This allows you to zoom in and out of the image up to 20X standard magnification, (40X for specific areas of interest). The system allows you to capture images and also enables you to preform automated analysis, so you can quantify and score your slides at the click of a button. Specialist Genie software also allows the recognition of different tissue types so that scoring can be based on specific areas within the slide.
For more information please contact Laura Wilson (email@example.com)
We have a dedicated immunohistochemistry laboratory which is located on the ground floor of the building. This room has all of the specialist machinery needed to process, embed and section human tissue ready for immunohistochemistry staining. This allows us to look at markers of interest within the tissue. Within this laboratory we also have the ability to do automated staining using the Ventana system, this system can be programmed to automate any staining that is usually preformed on the bench by hand therefore is more reproducible and quicker.
For more information please contact Callum Kirk (firstname.lastname@example.org)
World-leading research centre into childhood cancer officially opened by internationally-renowned author Bill Bryson, patron of the Future Fund.
This building was funded by the Wolfson Foundation, the Barbour Foundation, the Garfield Weston Foundation, the NECCR, and as part of the Future Fund campaign.
It was officially opened as The Wolfson Childhood Cancer Research Centre in September 2016 by Bill Bryson, patron of the Future Fund, and cost £5.5m.
Main focus of work in the building
The Wolfson Childhood Cancer Research Centre is a facility where clinical and research teams from across the city can come together to advance their understanding of how to treat childhood cancers. Work at the Wolfson Childhood Cancer Research Centre will focus on developing less toxic therapies with fewer side-effects, more effective treatment options for youngsters with advanced cancer and those whose illness has returned.
- Cell sorting using a BD Aria Sorter
- FACs Analysis (BD Canto and Calibur Machines)
- Real-time PCR
- 3D Digital PCR
- Fluorescent microscopes
- HTA compliant Sample storage
The facility now houses over 100 members of staff.