|Semester 1 Credit Value:||10|
To develop students’ knowledge of genome organisation and of the concepts and processes of prokaryotic and eukaryotic gene expression, to show how gene expression is currently believed to be regulated, and to illustrate the significance of this regulation for cell function and development; to introduce the use of computers to identify and analyse nucleic acid and protein sequences.
The study of DNA and proteins has influenced all aspects of biology, and understanding gene expression and its regulation is central to understanding how organisms develop and function. This module aims to develop students’ knowledge and appreciation of the genetic make-up of organisms - genome organisation - and the ways that genes are expressed and regulated in prokaryotes and eukaryotes. Computers are an essential tool for studying nucleic acids and proteins; the module introduces the use of a range of computer programs for identifying and analysing DNA and protein sequences.
- Review of fundamentals of gene expression.
- Transcription in eukaryotes and prokaryotes: mechanisms of transcription initiation, structure and function of promoters, RNA polymerases, transcription termination.
- The concept of the operon in prokaryotes and how operons are regulated.
- Sensing of the environment: the prokaryotic model.
- Organisation of eukaryotic genomes and genes; chromatin structure and its significance for transcription.
- Principles of transcription regulation in eukaryotes: interaction of transcription factors with DNA and with RNA polymerase; the importance of modularity and combinatorial regulation.
- Regulation of transcription by external signals such as hormones.
- Post-transcriptional regulation of gene expression.
- Subcellular targeting, secretion, and post-translational modification of proteins.
- Regulation of gene expression in eukaryotic development: embryo development, cell and tissue specialisation; Drosophila as model system.
- The evolutionary significance of changes in regulation of gene expression.
- Practical exercises: computer-based analysis of DNA sequences; interpretation of gene expression data.
|Scheduled Learning And Teaching Activities||Lecture||21||1:00||21:00||Includes 1hr exam practice|
|Guided Independent Study||Assessment preparation and completion||1||1:30||1:30||Final exam|
|Guided Independent Study||Assessment preparation and completion||17||1:00||17:00||Class test revision|
|Guided Independent Study||Assessment preparation and completion||20||0:30||10:00||Revision for final exam|
|Guided Independent Study||Assessment preparation and completion||4||1:00||4:00||Computer practical assessment|
|Scheduled Learning And Teaching Activities||Practical||2||2:00||4:00||2x 2hr computer practicals|
|Guided Independent Study||Skills practice||4||1:00||4:00||Computer practical follow-up homework|
|Guided Independent Study||Independent study||1||18:30||18:30||Study of lectures, ReCap, Blackboard etc.|
|Guided Independent Study||Independent study||20||1:00||20:00||Lecture follow up|
The lectures review background, explain key concepts and outline illustrative examples. A lecture will also introduce computer-based sequence analysis. In 4 lectures, progress is assessed and feedback is given through short quizzes. The class test provides students with an opportunity to answer a practice exam question. The final lecture is an optional review session. In the computer practicals, students use computers to analyse DNA and protein sequences with supervision and guidance. The practicals also include data interpretation exercises to reinforce students understanding of how gene expression is regulated. Private study is necessary for students to absorb information presented in lectures, to deepen their knowledge and understanding through reading supporting references, and to complete the sequence analysis and data interpretation exercises.
The format of resits will be determined by the Board of Examiners
|PC Examination||90||1||A||70||MCQs; 1 from 3 essays|
|Practical/lab report||1||M||20||Reports on computer practicals and gene expression data interpretation (2 x 10%)|
|Written exercise||1||M||10||Practice exam (2%), Quizzes (4 x 2%)|
The exam uses multiple choice questions (60%) and one essay question chosen from 3 (40%) to assess knowledge and understanding of the relevant subject material and the ability to integrate information from lectures and additional reading. The reports on the computer practicals and data interpretation exercises (deadlines weeks 6 and 9) test students' ability (a) to use computer programs to obtain information about DNA and proteins and to interpret this information in relation to their knowledge of molecular biology and (b) to analyse and interpret quantitative data on gene expression regulation. The quizzes (inTW 3, 5, 8 and 10) indicate progress and provide an incentive for sustained study. The practice exam question (week 11) gives students the opportunity and incentive to develop their exam technique in a situation with minimal pressure on them.
Disclaimer: The University will use all reasonable endeavours to deliver modules in accordance with the descriptions set out in this catalogue. Every effort has been made to ensure the accuracy of the information, however, the University reserves the right to introduce changes to the information given including the addition, withdrawal or restructuring of modules if it considers such action to be necessary.