Dr Joao Passos
Reader in Biology of Ageing
- Email: firstname.lastname@example.org
- Telephone: +44 (0) 191 208 1222
- Fax: +44 (0) 191 256 3445
- Personal Website: https://research.ncl.ac.uk/passoslab/
- Address: Edwardson Biogerontology Building
Institute for Cell and Molecular Biosciences
Campus for Ageing and Vitality
Newcastle upon Tyne
NE4 5PL, UK
João Passos is a lecturer at the Newcastle University Institute for Ageing and Institute for Cell and Molecular Biosciences. He graduated with a degree in Biochemistry from the University of Porto having spent a year conducting research at the Institute for Molecular and Cellular Biology in Porto. He then integrated the graduate program from Porto University, GABBA and obtained his PhD from Newcastle University in 2007.
Dr. Passos has made important contributions to understanding the impact of mitochondria and telomere dysfunction during cellular ageing. A major milestone was his 2007 paper in PLoS Biology. This work has thrown new light on the underlying causes of the marked stochastic variations seen in replicative senescence which involve mitochondria and telomeres. This work featured in The Scientist as a top article in ageing and was recommended by the Faculty of 1000.
In 2010 Dr. Passos’s scientific achievements and their potential were recognized by the award of the highly prestigious BBSRC David Phillips Fellowship. Since then, Dr. Passos has been running a highly active research group and is deputy lead of the “Mechanisms of ageing theme” at the Newcastle University Institute for Ageing. His group is investigating mechanisms involved in maintenance of cellular senescence in particular the role of mitochondria and telomeres both in vitro and in vivo. His group’s paper published in 2012 in Nature Communications demonstrates how cellular senescence depends on chronic DNA-damage signaling from irreparable damage to telomeres. Dr. Passos has published more than 35 papers in leading scientific periodicals on the subject of biology of ageing and is associate editor for 3 of the top journals in ageing research: Aging Cell, Mechanisms of Ageing and Development & Biogerontology. He also serves in the BBSRC panel.
Research and Funding:
Joao Passos's Lab is investigating mechanisms involved in maintenance of cellular senescence in particular the role of mitochondria and telomeres both in vitro and in vivo.
Dr. Passos is PI in several grants investigating different mechanisms underlying cellular senescence (funded by BBSRC and BHF) and PhD studentship funded by BBSRC and MRC including a BBSRC/Unilever funded PhD Industrial CASE studentship.
He is part of the MRC-Arthritis Research UK Centre for Integrated research into Musculoskeletal Ageing (CIMA) involving the Universities of Liverpool, Newcastle and Sheffield and the MRC centre CAV.
He is also a PI in the ￡3M FP7 European grant MARie CuRIe AGEing Network led by the University of Groningen.
Dr. Passos is very active in engagement activities and has encouraged a culture of public engagement within his research group:
JP participated as a judge in “Debating Matters” involving school children debating about the impact of an ageing population on society; JP did a Café Scientifique on the subject of Biology of Ageing organized by the British Council; Rhys Anderson a PhD student from JP’s lab recently won the “People’s choice award” at the prestigious “3 minute thesis competition” which allowed him to produce an animation video of our research: https://www.youtube.com/watch?v=SR3MJYbOmKo; members from JP’s lab participated in events such as: Healthy ageing: live better for longer! at the BODY WORLDS Vital exhibition and The project Old Vic NewVoices where a community play about ageing is being developed).
Dr. Passos teaches at the Biology of Ageing MRes course. He also supervises several undergraduate, MRes and PhD students in his lab.
- Hewitt G, Jurk D, Marques FDM, Correia-Melo C, Hardy T, Gackowska A, Anderson R, Taschuk M, Mann J, Passos JF. Telomeres are favoured targets of a persistent DNA damage response in ageing and stress-induced senescence. Nature Communications 2012, 3(2), 708.
- Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender SLF, Fullard N, Nelson G, Mann J, van de Sluis B, Mann DA, von Zglinicki T. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nature Communications 2014, 2, 4172.
- Birch J, Anderson RK, Correia-Melo C, Jurk D, Hewitt G, Marques FM, Green NJ, Moisey E, Birrell MA, Belvisi MG, Black F, Taylor JJ, Fisher AJ, De Soyza A, Passos JF. DNA damage response at telomeres contributes to lung aging and chronic obstructive pulmonary disease. American Journal of Physiology: Lung Cellular and Molecular Physiology 2015, 309(10), L1124-L1137.
- Passos JF, Nelson G, Wang CF, Richter T, Simillion C, Proctor CJ, Miwa S, Olijslagers S, Hallinan J, Wipat A, Saretzki G, Rudolph KL, Kirkwood TBL, von Zglinicki T. Feedback between p21 and reactive oxygen production is necessary for cell senescence. Molecular Systems Biology 2010, 6(1), 347.
- Wilson CL, Jurk D, Fullard N, Banks P, Page A, Luli S, Elsharkawy AM, Gieling RG, Bagchi-Chakraborty J, Fox C, Richardson C, Callaghan K, Blair GE, Fox N, Lagnado A, Passos JF, Moore AJ, Smith GR, Tiniakos DG, Mann J, Oakley F, Mann DA. NFkB1 is a suppressor of neutrophil-driven hepatocellular carcinoma. Nature Communications 2015, 6, 6818.
- Passos JF, Saretzki G, Ahmed S, Nelson G, Richter T, Peters H, Wappler I, Birket MJ, Harold G, Schaeuble K, Birch-Machin MA, Kirkwood TBL, von Zglinicki T. Mitochondrial dysfunction accounts for the stochastic heterogeneity in telomere-dependent senescence. PLoS Biology 2007, 5(5), 1138-1151.
- Lawless C, Jurk D, Gillespie CS, Shanley D, Saretzki G, von Zglinicki T, Passos JF. A Stochastic Step Model of Replicative Senescence Explains ROS Production Rate in Ageing Cell Populations. PLoS One 2012, 7(2), e32117.
- Lawless C, Wang CF, Jurk D, Merz A, von Zglinicki T, Passos JF. Quantitative assessment of markers for cell senescence. Experimental Gerontology 2010, 45(10), 772-778.
- Passos J, Saretzki G, von Zglinicki T. DNA damage in telomeres and mitochondria during cellular senescence: Is there a connection?. Nucleic Acids Research 2007, 35(22), 7505-7513.
- Passos JF, von Zglinicki T. Mitochondrial dysfunction and cell senescence - skin deep into mammalian aging. Aging 2012, 4(2), 74-75.
- Neganova I, Vilella F, Atkinson SP, Lloret M, Passos JF, von Zglinicki T, O'Connor JE, Burks D, Jones R, Armstrong L, Lako M. An Important Role for CDK2 in G1 to S Checkpoint Activation and DNA Damage Response in Human Embryonic Stem Cells. Stem Cells 2011, 29(4), 651-659.
- Ahmed S, Passos JF, Birket MJ, Beckmann T, Brings S, Peters HH, Birch-Machin MA, von Zglinicki TW, Saretzki GC. Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress. Journal of Cell Science 2008, 121(7), 1046-1053.
- Passos JF, Von Zglinicki T, Kirkwood TBL. Mitochondria and ageing: Winning and losing in the numbers game. BioEssays 2007, 29(9), 908-917.
- Soleimanpour-Lichaei HR, Kuhl I, Gaisne M, Passos JF, Wydro M, Rorbach J, Temperley R, Bonnefoy N, Tate W, Lightowlers R, Chrzanowska-Lightowlers Z. mtRF1a Is a Human Mitochondrial Translation Release Factor Decoding the Major Termination Codons UAA and UAG. Molecular Cell 2007, 27(5), 745-757.
- Passos JF, Nelson G, von Zglinicki T. Telomeres, Senescence, Oxidative stress and heterogeneity. In: Rudolph, KL, ed. Telomeres and Telomerase in Aging, Disease, and Cancer: Molecular Mechanisms of Adult Stem Cell Ageing. Berlin: Springer, 2008, pp.43-56.
- Ivanov A, Pawlikowski J, Manoharan I, vanTuyn J, Nelson DM, Rai TS, Shah PP, Hewitt G, Korolchuk VI, Passos JF, Wu H, Berger SL, Adams PD. Lysosome-mediated processing of chromatin in senescence. Journal of Cell Biology 2013, 202(1), 129-143.
- Correia-Melo C, Passos JF. Mitochondria: Are they causal players in cellular senescence?. Biochimica et Biophysica Acta (BBA) - Bioenergetics 2015, 1847(11), 1373-1379.
- Neganova I, Tilgner K, Buskin A, Paraskevopoulou I, Atkinson SP, Peberdy D, Passos JF, Lako M. CDK1 plays an important role in the maintenance of pluripotency and genomic stability in human pluripotent stem cells. Cell Death and Disease 2014, 5, e1508.
- Tarrago MG, Chini CCS, Kanamori KS, Warner GM, Caride A, deOliveira GC, Rud M, Samani A, Hein KZ, Huang R, Jurk D, Cho DS, Boslett JJ, Miller JD, Zweier JL, Passos JF, Doles JD, Becherer DJ, Chini EN. A Potent and Specific CD38 Inhibitor Ameliorates Age-Related Metabolic Dysfunction by Reversing Tissue NAD+ Decline. Cell Metabolism 2018, 27(5), 1081-1095.
- Birch J, Barnes PJ, Passos JF. Mitochondria, telomeres and cell senescence: Implications for lung ageing and disease. Pharmacology and Therapeutics 2018, 183, 34-49.
- Carroll B, Otten E, Manni D, Stefanatos R, Menzies F, Smith G, Jurk D, Kenneth N, Wilkinson S, Passos J, Attems J, Veal E, Teyssou E, Seilhean D, Millecamps S, Eskelinen E-L, Bronowska A, Rubinsztein DC, Sanz A, Korolchuk V. Oxidation of SQSTM1/p62 mediates the link between redox state and protein homeostasis. Nature Communications 2018, 9, 256.
- de Magalhaes JP, Passos JF. Stress, cell senescence and organismal ageing. Mechanisms of Ageing and Development 2018, 170, 2-9.
- Ogrodnik M, Miwa S, Tchkonia T, Tiniakos D, Wilson CL, Lahat A, Day CP, Burt A, Palmer A, Anstee QM, Nagaraja Grellscheid S, Hoeijmakers JHJ, Barnhoorn S, Mann DA, Bird TG, Vermeij WP, Kirkland JL, Passos JF, vonZglinicki T, Jurk D. Cellular senescence drives age-dependent hepatic steatosis. Nature Communications 2017, 8, 15691.
- Schafer MJ, White TA, Iijima K, Haak AJ, Ligresti G, Atkinson EJ, Oberg AL, Birch J, Salmonowicz H, Zhu Y, Mazula DL, Brooks RW, Fuhrmann-Stroissnigg H, Pirtskhalava T, Prakash YS, Tchkonia T, Robbins PD, Aubry MC, Passos JF, Kirkland JL, Tschumperlin DJ, Kita H, LeBrasseur NK. Cellular senescence mediates fibrotic pulmonary disease. Nature Communications 2017, 8, 14532.
- Correia-Melo C, Ichim G, Tait SWG, Passos JF. Depletion of mitochondria in mammalian cells through enforced mitophagy. Nature Protocols 2017, 12(1), 183-194.
- Salmonowicz H, Passos JF. Detecting senescence: a new method for an old pigment. Aging Cell 2017, 16(3), 432-434.
- Anderson R, Richardson GD, Passos JF. Mechanisms driving the ageing heart. Experimental Gerontology 2017, (ePub ahead of Print).
- Birch J, Passos JF. Targeting the SASP to combat ageing: Mitochondria as possible intracellular allies?. BioEssays 2017, 39(5), 1600235.
- Victorelli S, Passos JF. Telomeres and Cell Senescence - Size Matters Not. EBioMedicine 2017, 21, 14-20.
- Carroll B, Maetzel D, Maddocks ODK, Otten G, Ratcliff M, Smith GR, Dunlop EA, Passos JF, Davies OR, Jaenisch R, Tee AR, Sarkar S, Korolchuk VI. Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity. eLIFE 2016, 5, e11058.
- Neganova A, Shmeleva E, Munkley J, Chichagova V, Anyfantis G, Anderson R, Passos AJ, Elliott DJ, Armstrong L, Lako M. JNK/SAPK signalling is essential for efficient reprogramming of human fibroblasts to induced pluripotent stem cells. Stem Cells 2016, 34(5), 1198-1212.
- Correia-Melo C, Marques FDM, Anderson R, Hewitt G, Hewitt R, Cole J, Carroll BM, Miwa S, Birch J, Merz A, Rushton MD, Charles M, Jurk D, Tait SWG, Czapiewski R, Greaves L, Nelson G, Bohlooly-Y M, Rodriguez-Cuenca S, Vidal-Puig A, Mann D, Saretzki G, Quarato G, Green DR, Adams PD, von Zglinicki T, Korolchuk VI, Passos JF. Mitochondria are required for pro-ageing features of the senescent phenotype. EMBO Journal 2016, 35(7), 724-742.
- Correia-Melo C, Birch J, Passos JF. Powering senescence: The ugly side of mitochondria. Cell Cycle 2016, 15(19), 2541-2542.
- Hewitt G, Carroll B, Sarallah R, Correia-Melo C, Ogrodnik M, Nelson G, Otten EG, Manni D, Antrobus R, Morgan BA, von Zglinicki T, Jurk D, Seluanov A, Gorbunova V, Johansen T, Passos JF, Korolchuk VI. SQSTM1/p62 mediates crosstalk between autophagy and the UPS in DNA repair. Autophagy 2016, 12(10), 1917-1930.
- Birch J, Victorelli S, Rahmatika D, Anderson RK, Jiwa K, Moisey E, Ward C, Fisher AJ, De Soyza AJ, Passos JF. Telomere Dysfunction and Senescence-associated Pathways in Bronchiectasis. American Journal of Respiratory and Critical Care Medicine 2016, 193(8), 929-932.
- Anderson R, TualChalot S, Redgrave R, Dodds R, Owens WA, Saretzki G, Arthur H, vonZglinicki T, Passos JF, Richardson GD. The role of cardiomyocyte senescence and regeneration in Ageing. In: British Microcirculation Society 66th Annual Meeting. 2016, Newcastle upon Tyne, UK: BMS.
- Hewitt G, Von Zglinicki T, Passos JF. Cell sorting of young and senescent cells. Methods in Molecular Biology 2013, 1048, 31-47.