Bioimaging Unit

Zeiss AxioObserver

Zeiss AxioObserver Spinning Disk

Modalities: invert, confocal, live cell

Overview

Spinning disk microscopy is an established imaging technique of choice for imaging both dynamic and light-sensitive processes in living cells.

The system utilises the Yokogawa CSUX1 spinning disk head and coupled with a QuantEM camera (Photometrics) capable of capturing high speed (>30fps) confocal data sets.

The microscope employs software autofocus correction and is fully-enclosed in a heated environmental chamber with the ability to set, maintain and monitor:

  • temperature
  • humidity
  • O2 levels
  • CO2 levels

as imaging conditions dictate. The system is run through Zeiss’ AxioVision software package.

Imaging modes

  • Multipoint x,y with tiling (2D),
  • volume x,y,z (3D), 
  • fluorescence, 
  • FRET, 
  • DIC, t
  • ime-lapse x,y,z,t (4D)
Spinning Disc

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification

NA

Coverslip

Other modes

Immersion
10 0.3 0.17   Air
20 0.8     Air
40 1.3 0.17   Oil
100 1.4 0.17   Oil

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part Number
DAPI DAPI, Hoechst        
FITC

FITC+AF488

        
TRITC          

 

 

 

 

 

 

 

Laser lines

405 nm, 488nm, 561nm, 639 nm

Location

Leech Building, M2.143

Medical School
Newcastle University
Newcastle upon Tyne
NE2 4HH

Enter your postcode or address to find directions

Booking

Find out how to book this microscope.

Booking information 

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use. 
Make a Booking

Training

Here you find useful training videos for the system.

Zen Blue software tutoial. 

Explore Zen.

SOP

Zeiss AxioImager (Zen Blue)

SOP

Zeiss AxioImager (Zen Blue)