Bioimaging Unit

Zeiss AxioPlan 2 BF

Zeiss AxioPlan 2 BF

Modalities: upright, widefield

Overview

The Zeiss Axioplan imaging system is a universal microscope which is suitable for several applications relevant to light microscopy.

This system has a manual stage with Axiocam HRc camera for Brightfield or Histology imaging only.

Imaging modes

  • Brightfield
  • Phase contrast
Zeiss Axioplan 1 BF (CfL)

Specification

The Zeiss AxioPlan offers three modes of operation: brightfield transmitted light and wide-field fluorescence.

Objectives

Magnification

NA

Coverslip

Other modes

Immersion

1.25 0.03     Air
2.5 0.075     Air
5 0.15 0.17 Ph1 Air
10 0.3 0.17 Ph1 Air
20 0.5 0.17 Ph2 Air
40 0.75 0.17 Ph2 Oil
63 1.4 0.17 Ph3 Oil

 

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube

Example fluorophores

Excitation

Dichroic

Emission

Part Number

Zeiss 02 DAPI, Hoechst 335-390 395 420 (LP)  
Zeiss 09 HE

FITC+AF488

450-490 510 515 (LP)  
Zeiss 13 eGFP 460-480 495 505-530  

 

 

 

 

 

Light source (epi)

None for epi

Detectors, cameras

Zeiss AxioCam HRc (Colour camera used for both fluorescence and brightfield HC)

 

Location

East Wing, International Centre for Life, room G329

Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ

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Booking

Find out how to book this microscope.

Booking information

  • The minimal booking time for this system is 30 minutes. 
  • Time increments of 15 minutes are permitted. 
  • Bookings can be cancelled with no charge up to an hour before scheduled use.